羊痘

This assay provided basis for the establishment of rapid and available goat and sheep pox diagnosis methods and for the study of CPV molecular properties.

本试验为羊痘病快速有效诊断方法的建立和CPV的分子生物学特性的研究奠定了基础。

Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

Western blot试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

The expressed protein accounted for more than 30 % of the total protein mass. Western blot confirmed that the fusion protein reacted to positive serum against sheep pox.

在IPTG 浓度为0.1 mM、温度为37 ℃、诱导6 h时表达量最大，约占菌体蛋白的30% Western blot 试验表明，融合蛋白可被羊痘阳性血清识别，这为研究其在新型疫苗和诊断的研究奠定了基础。

The glycoprotein-encoding ORF117 gene was cloned by PCR from genomic DNA of sheep pox virus isolated in Jingtai, Gansu Province.

以甘肃景泰疑似羊痘患病绵羊的皮肤组织中提取的总DNA 为模板，经PCR 扩增获得SP 病毒 ORF117 基因。

Capripoxvirus was the agent of goat and sheep pox disease.

羊痘病毒(Capripoxvirus，CPV)是山羊痘病和绵羊痘病的病原。

The results of sequencing show that GTPV P32 gene was 969bp and encode 323 amino acids, Sheep pox virus P32 gene was 972bp and encode 324 amino acids, FGD-CPV P32 gene was 969bp and encode 323 amino acids.

结果表明：GTPV P32基因开放阅读框全长969bp，编码323个氨基酸；绵羊痘病毒(Sheep pox virus，SPPV)P32基因ORF全长972bp，编码324个氨基酸；羊胎皮肤细胞分离毒P32基因ORF全长969bp，编码323个氨基酸。

Pox formation increased from 13.3%-20% to 33.3%-40% after passaging through 9 days old chicken embryo. Typical virus particles were observed under transmission electronic microscope in ultrathin sections of pathological tissues, allantochorion membrane of chicken embryos and affected cells.

用待检病料感染的BHK-21细胞培养物接种9日龄鸡胚绒毛尿囊膜，随着传代次数的增加，痘斑病变的出现率从13.3%~20%上升至33.3%~40%；用山羊痘病变皮肤、鸡胚绒毛尿囊膜和感染细胞进行超薄切片，在电子显微镜下可以观察到典型的山羊痘病毒粒子。

The purpose of the study is to construct recombinant goat pox virus expressing Peste des petits ruminants virus H protein, and to evaluate the immunization effect.

本研究旨在构建表达小反刍兽疫H蛋白的重组山羊痘病毒，并评价其免疫效力。

The inverted terminal repeat gene fragments of goat poxvirus QL、LD、Y、B-strain were multiplied by PCR and cloned into the pMD18-T vector.

山羊痘是由羊痘病毒属山羊痘病毒(Goat poxvirus，GPV)引起山羊及绵羊的一种急性、热性、高度接触性传染病[1]。

The fluorescent antibody was prepared with IgG purified from rabbit anti-goatpox virus serum and used to detect the antigen of GPV in pock samples and BHK-21 cells infected respectively with GPV strains B,Y,LD and QL.

用兔抗山羊痘病毒IgG制备荧光抗体，检测了临床发病山羊皮肤痘疹切片和山羊痘病毒B、Y、LD和QL株感染的BHK-21细胞中的病毒抗原。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。