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The genome DNA was amplied and electrophoresis of Sichuan 7 native goat breeds and1 cultivated breed, including Tibet goat(n=27)、ChengDu Ma goat(n=20)、JinTang blackgoat(n=21)、LeZhi black goat(n=24)、JianChang black goat(n=23)、BeiChuan white goat(n=26),Jian Yang Daer goat(n=30) and NanJiang yellow goat (n=90), using 5 pairs of microsatelliteprimers.

选用5对微卫星引物,对四川7个地方山品种—简阳大耳(n=30)、建昌黑山(n=23)、成都麻(n=20)、藏山(n=27)、金堂黑山(n=21)、乐至黑山(n=24)和北川白山(n=26)及1个培育品种—南江黄(n=90)的基因组DNA进行PCR扩增,并进行电泳分析。

The phylogeny tree indicated that Tibet goat, ChengDugoat, JinTang black goat and Beichuan white goat formed a branch, and LeZhi black goat andJianChang black goat formed a branch, and the next was Jian Yang daer goat.

以Nei's遗传距离为基础构建的系统发生树表明,藏山和金堂黑山、成都麻、北川白山聚为一类,乐至黑山和建昌黑山聚为一类,而简阳大耳自成一类。

Result150of patients and infected sheep,Cattle,Swine serum positive rates of DAgS -ELISA were 91.4%,74.6%,and 66.7%re spective-ly. In the main, DAgS -ELISA gain adva ntage over of SAT and RBPT.

结果上述3种试验对健康人、、牛、猪共计140份血清检查均为阴性反应,用DAgS-ELISA对布氏菌感染人、、牛、猪共计150份血清检查,其阳性率分别为91.4%、74.6%、66.7%及66.7%,从总体而言,检查的特异性及敏感性,以DAgS-ELISA优于SAT及RBPT。

A double antigens Sandwich Enzymelinked Immunosor-bent Assay( DAgS -ELISA)have been developed under the basis o f higher titer and freeze dried production conjugate of Brucella antigen with horseradish peroxidase. Serumsamples formdiagnosed patients of Brucellosis and sheep,cattl e,swine infected with Bru-cella as well as normal men and those a nimals in laboratory detected throu gh DAgS -ELISA,SAT and RBPT tests.

方法在制备高滴度布氏菌抗原辣根过氧化物酶结合物及其冻干制品基础上,应用酶联免疫吸附双抗原夹心法即双抗原夹心酶联免疫吸附试验、试管凝集试验及虎红平板凝集试验对从山西省布氏菌病疫区收集的布氏菌病患者、布氏菌感染、牛、猪以及健康人、、牛、猪共计290份血清进行对比检测。

But experts said that this is a deformational sheep, and the possibility to breed the 4-horned sheep is very small.

但专家断定这是一只畸形,繁殖出四角后代的可能性极小。

While he was still talking with them, Rachel came with her fat her 's sheep, for she was a shep her dess .

雅各正和他们说话的时候,拉结领着她父亲的来了,因为那些是她牧放的。

With 50% ethanol to elute to icarrin and then with Ethyl Acete and 60% ethanol to crystallize, the purity of icariin reached 90.02%.

用50%乙醇洗脱淫藿苷,经乙酸乙酯和60%乙醇重结晶后,干燥物中淫藿苷的含量达90.02%。

All IBDV BC 6/85 F4 and IBDV HE BEI isolated strain were obtained from allantochonon and allantoic fluid of died embryonated eggs in 2-5 days after inoculating and virus-infected cell fluid that were frozen and thawed three times after approximately 80% of the monolayer exhibited cytopathic effects.

将纯化的IBDV分别免疫家兔和小鼠,制备了兔抗IBDV、鼠抗IBDV的高免血清,用健康兔的血清免疫而获得抗兔免疫血清。

Objective:To optimize the processing condition of electric roasting Epimedium of goats fat.

目的 :优选脂油炙淫藿电烤工艺的最佳炮制条件。

Epimedium and Icariin have estrogenlike effects and all of their effects are realized through the estrogen receptor.

藿及淫藿苷具有雌激素样作用,且其促细胞增殖作用可能是通过雌激素受体介导产生的。

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