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Additionally,this study developed three species-spcific real-time fluorescence quantitative PCR-based assays for rapidly, specificity, sensitivity,detecting Brucella abortus, Brucella melitensis, Brucella suis.

在建立布鲁氏菌属荧光定量PCR检测方法的基础上,根据布鲁氏菌基因组多拷贝插入元件IS711及其下游多态位点,设计了检测牛、、猪种布鲁氏菌的引物和牛、、猪种特异性荧光探针,优化了牛、、猪种布鲁氏菌荧光定量PCR反应体系和条件,建立了牛、、猪种布鲁氏菌荧光定量PCR检测方法,组装了牛、、猪种布鲁氏菌检测试剂盒,该方法能特异检测上述3个种的布鲁氏菌。

In the "Yang Yang Games" in, revealing the warm Yang Yang was originally argali, which is a huge reason for her size.

在《运动会》中,揭示了暖原来是盘,这也是她体型巨大的原因。

Euler early after the sheep and Tibetan wild argali sheep constantly cross-breeding of the skin after the formation of a combination of high-quality goat type species, with strong disease resistance, cold resistance, fitness, and growth and development of fast, high-volume production of meat, fresh meat No smell of mutton taste, commodity rates, operation agility, teamwork and strong resistance, feeding coarse, easily bred and hides, and skin thickness, strong, flexible, and strong features such as heat, is the best skin products market.

欧拉是早期经过野生盘和藏系不断杂交,繁育后形成的皮肉兼用型优质种,具有抗病力强,耐高寒,体格大,生长发育快、产肉量高、肉质鲜嫩无膻味,商品率高,行动敏捷,合群性强,耐粗饲、易饲养及皮张大,皮板厚,结实、柔韧性好、保暖性强等特点,是皮肉市场中的上乘品。2005年在首届青海高原特色农畜产品展示会上被评为优质畜种。

Blood samples from 23 Huacaya alpacas ,3 males and 20 females ,were used to study chromosome karyotype ,so as to provide some effective cytogenetics basis for the selectioiymprovement by crossing and disease diagnosis of alpacas ,and genetic mechanisms of sex determination. Peripheral blood lymphocyte culture was used to prepare chromosome. Methods of trypsase-EDTA and BSG (Barium hydroxide / Saline / Giemsa)were used for G-banding and C-banding respectively . The results showed as follows:Firstly , the number of diploid chromosomes was in the alpacas 2n=74,with the karyotype 74, XY and 74, XX for males and females respectively.

为了从选种、杂交改良、疾病诊断以及性别决定的遗传机制等方面为驼的繁育与推广提供更为有效的细胞遗传学资料,本试验采用外周血淋巴细胞培养法制备染色体,分析了23只胡阿基亚型驼(Huacaya alpaca,雌20只,雄3只)的染色体及其核型,并通过BSG法(Barium hydroxide/Saline/Giemsa,氢氧化钡-盐溶液-吉姆萨,也即Sumner BSG法)与胰酶-EDTA法分别对驼的C-分带和G-分带进行分析,结果表明:(1)驼二倍体染色体数目为2n=74,雄性驼核型为74,XY;雌性驼核型为74,XX。

China's civil law has Fresh: Lushun in Liaoning Province and mussels along the sargassum cooked together;×÷sargassum Rongcheng, Shandong Province steaming bags, Rushan sargassum boiled tofu; Fujian coastal sargassumówith the fish, cut after cooling;Sargassum area, Wenzhou, Zhejiang Province.

我国民间食法有:辽宁省旅顺一带栖菜和贻贝拼煮;山东省荣成栖菜作馅蒸包,乳山栖菜煮豆腐;福建省沿海栖菜与鱼共煮,冷却后切块;浙江省温州一带栖菜炒肉丝。

Kefir manufactured by goat milk powder had significantly higher medium chain fatty acid than by fresh goat milk. This could be explained by the higher fat content in goat milk powder. Addition of growth promoters decreased the level of medium chain fatty acids. By comparing both growth promoters, adding peptide had significantly lower caproic acid and caprylic acid than isomaltooligosaccharide did.

乳克弗尔中链脂肪酸之研究结果显示,乳粉制作的乳克弗尔的油酸、脂酸及葵酸含量均比鲜乳的含量高:克弗尔的中链肪酸随著添加物之不同而有所改变,整体来说,以不添加任何生长促进物的对照组其C6、C8和C10脂肪酸含量为高,添加肽类组最低。

The results showed that there was a mutation of A → G in BMPR-IB gene in Cele sheep,Duolang sheep,Chinese Merino monotocous line and Hu sheep,which were the same as in Booroola Merino ewes.

结果表明,在策勒、多浪、中国美利奴多胎类型和湖等品种中都存在和Booroola Merino相同的BMPR-IB基因的突变(A746G),而在中国美利奴中没有发现相应的突变。

Amino acid similarities between goat and human were 87% and between gaot and mouse were 83%. Just like other members of FGFs superfamily, FGF5 protein also has a signal sequence ,at same time its protein has higly similar sequence about 120 Amino acid in central core sequence with other breed. Tissue specific expression of the FGF5 gene was also investigated in cashmere goat by RT-PCR analysis. The results showed that FGF5 gene was expressed in different time of the tested skin tissues, including anage and telegon.

结果如下:(1)本研究利用 RT-PCR 方法克隆了 FGF5 基因的 cDNA 序列,得到了 925bp的序列,并对该序列进行了分析,与成纤维细胞生长因子家族的成员一样也是由三个外显子组成,也具有一信号序列,成熟蛋白为 270 个氨基酸,经同源性比对得出大鼠与小鼠的 FGF5 氨基酸同源性最高为 97%,在与的蛋白质比较中大鼠与的同源性为 83%,小鼠与的同源性为 84%,人与的同源性最高,为 89%。

Four little human run over. They talk loudly and nosey .One little human surprisingly catch Little Sheep. Little Sheep struggle and try to run away. He kick with his two legs in the back. That little human fell down. He hurt his knee and cry. The other three start to throw stone to Little Sheep. Little Sheep run and run.

四个小人类跑过,讲话的声音大声且刺耳,他们朝小跑来,一个小人类冷不防的将小给抓住,小挣扎著,后腿一踢,那小人类被跌倒在地上,他擦破膝盖,痛哭起来,其他三人开始朝小丢石头,小一路跑著,三个小人类在后头紧追不舍。

She is revealed in the Olympics special to possess mountain goat genes, effectively making her the strongest goat in Goat Village above Fit Goat.

在奥运特辑中,发现她原来也有盘的基因,结果她就超越沸成为村最强壮的了。

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Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

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