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Antigene peptide nucleic acid specifically inhibits MYCN expression in human neuroblastoma cells leading to cell growth inhibition and apoptosis Roberto Tonelli,Stefania Purgato,Consuelo Camerin,Raffaele Fronza,Fabrizio Bologna,Simone Alboresi,Monica Franzoni,Roberto Corradini,Stefano Sforza,Andrea Faccini,Jason M. Shohet,Rosangela Marchelli,and Andrea Pession Department of Pediatrics, University of Bologna,Sant'Orsola-Malpighi Hospital, Bologna, Italy; Department of Organic and Industrial Chemistry, University of Parma, Parma,Italy; and 3Center for Cell and Gene Therapy, Texas Children's Cancer Center, Baylor College of Medicine, Houston, Texas Abstract We developed an antigene peptide nucleic acid for selective inhibition of MYCN transcription in neuroblastoma cells, targeted against a unique sequence in the antisense DNA strand of exon 2 of MYCN and linked at its NH2 terminus to a nuclear localization signal peptide.

抗原肽核酸抑制MYCN具体表达的神经母细胞瘤细胞,导致细胞生长的抑制和凋亡罗伯托利,斯特凡Purgato ,苏埃洛卡默琳,拉菲尔Fronza ,梅奥尼博洛尼亚,西蒙娜Alboresi ,莫妮卡弗兰索尼,罗伯托Corradini ,斯特凡诺斯福尔扎,安德烈Faccini ,杰森先生Shohet , Rosangela Marchelli ,和安德烈Pession儿科,博洛尼亚大学,圣' Orsola -马尔皮基医院,博洛尼亚,意大利;有机部和工业大学化学帕尔马,帕尔马,意大利和3Center的细胞和基因治疗,德州儿童癌症中心, Baylor医学院,得克萨斯州休斯敦摘要我们开发了一种抗原肽核酸的选择性抑制神经母细胞瘤MYCN转录细胞,针对一个独特的序列反义DNA链的第2外显子,并与在MYCN 其氨基端为核定位信号肽。

He has premiered many works written by Aldo Clementi, Fabio Vacchi, Alessandro Solbiati, and several other composers. He also recorded Muzio Clementi Studi "Gradus ad Parnassum" for the German label "Arts".

阿尔多·克莱门蒂、法比奥·瓦奇、阿里桑德罗·索比亚蒂等很多作曲家的作品都是首次由罗伯托演奏的,他还与德国的Arts录制了缪奇奥·克莱门蒂·斯塔蒂的"通向希腊之山"。

Roberto is also an appreciated musicologist and has recently won the forst prize at the T.I.M.

罗伯托·普罗赛达同时也是一位出色的音乐学者。

His quickness and playmaking ability in the back proved to be an instant asset to Brazil.

他的高速奔跑和组织能力成为了巴西队中的宝贵财富。1995年罗伯托·卡洛斯的价值得到了极大的公认并登陆欧洲赛场,他从帕尔梅拉斯队转会到了国际米兰,但卡洛斯在圣西罗球场的日子并不长,英格兰籍主教练霍奇森将他的位置前提到了中场,但这个创举却令卡洛斯无所适从。

At the same time, and set up factories in Qingdao泰纳瑞斯process, the Roberto Rocca Education Program to China.

同时,泰纳瑞斯在青岛建厂过程中,将罗伯托·罗卡教育计划带到了中国。

City 2 "211 Project" institutions and China Ocean University of China University of Petroleum, Roberto Rocca Education Program to become the first cooperation between the domestic colleges and universities.

我市两所"211工程"院校中国海洋大学和中国石油大学成为罗伯托·罗卡教育计划首次合作的国内高校。

The Wigan manager, Roberto Martinez, described it as "unsporting" and the FA's disciplinary department asked Webb to review the television footage to ascertain whether he had seen the incident and, if not, whether he believed it constituted violent conduct.

维根经理罗伯托。马丁内斯认为这是非体育行为,足总纪律委员会让韦伯看录像确定他当时是否看到了那个事件。如果没有的话,他是否认为那是暴力事件。

Your first match was at home to Vicenza...

"记得当时是罗伯托·卡洛斯打入了唯一一球,那是我第一次在这个伟大的圣西罗球场中的比赛,从此开始了我的国米生涯。"

Addressing the upper house of the Italian parliament late Tuesday, Interior Minister Roberto Maroni praised the work of the security forces in the southern Calabrian town of Rosarno, where African farm workers rioted in the streets last week.

周二晚些时候,内政部长罗伯托-马罗尼向议会上院发表讲话。他赞扬了卡拉布里亚区南部Rosarno地区安全力量的工作。上周,非洲农田工作人员在这里引发了暴动。

Back on the pitch, Ribéry and Karim Benzema missed opportunities to double the advantage when one-on-one with the Colombia keeper after swift breakaways.

比赛中,里贝里和本泽马随后都错失了几次绝佳的单刀进球机会,法国队门将库佩则把罗伯托-波罗的远射挡下。

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