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网织红细胞

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The amount of vimentin IF per cell was higher than those of tubulin and F-actin at 12-24h of culture, but it broken down and decreased steadily when the cells became differentiated into late erythroblast at 36-48h of culture, thus facilitated the eccentric nucleation which we regard it as the initial step of denucleation. Both of the fluorescence intensity of tubulin and actin exhibited a significant rise and aggregated between the extruding nucleus and incipient reticulocyte before and during denucleation, and finally play a role in the commitent to ennucleation, the second phase of denucleation.

在培养24小时前,每个细胞中波形纤维含量均高于同期微丝和微管蛋白含量,但在培养24-36小时的晚幼红细胞阶段,波形纤维蛋白降减和解聚,为核偏位创造了条件,这是排核的第一阶段,而随后微丝和微管蛋白的荧光染色强度则有所增加,并逐渐聚集在外排中的核与预期形成网织红细胞之间的缢痕区,在将细胞核排出的排核第二阶段起主要作用。

Methods The Hb, Hct, Plt, and Ret were examined in patients before and after hemospasia, after transfusion and before discharge, respectively.

于采血前、采血后、回输血后、出院前检测病人红细胞、血红蛋白、白细胞、血小板、网织红细胞、血细胞比容的变化。

MAIN OUTCOME MEASURES: Variables as followings were measures: blood routine examination indexes, blood sedimentation, lactic acid dehydrogenase, C reactive protein, lymphocyte proportion, reticulocyte, mean corpuscular volume, febrile, hemorrhage, arthralgia, intumescence, edema and erythra. RESULTS: A total of 92 patients (86%) developed diseases of the hematopoietic system.

主要观察指标:考察变量为血常规3项、血沉、乳酸脱氢酶、C反应蛋白、淋巴细胞比例、网织红细胞、平均红细胞体积、发热、出血、骨关节痛、肿大、水肿及皮疹等15项指标。

The reticulocyte is not completely mature red blood cell; it is the precursor of mature erythrocyte.

网织红细胞是尚未完全成熟的红细胞,是成熟红细胞的前体。

Objective To acquire more and exact informations,red blood cell and reticulocyte indices were determined.

目的通过同时测定红细胞参数和网织红细胞参数,以利用有限数据为患者提供更多、更为准确的信息。

Objective To investigate the reference value range of automated reticulocyte multiple parameters analysis in Chinese healthy people,and to discuss changes and clinical significances of these parameters in thalassemia patients.

目的 调查健康中国成人网织红细胞多参数分析的参考值范围,并进一步观察地中海贫血时网织红细胞各参数的改变情况及其临床意义。

It was found that the membrane shear elastic modulus and membrane viscosity decreased in this process of changing. This finding may have some basic theoretical and clinical significance.

这对研究由于贫血等原因造成的网织红细胞增多情况下全血的微观流变学特性有重要的临床意义,同时对新生网织红细胞在转化过程中膜的剪切弹性模量和表面粘度的变化规律加以系统研究,具有重要的基础理论研究价值。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪,coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较,对130份正常人标本进行网织红细胞测定。结果:f=3.87,p.05,差异有显著性。其两两之间r值分别是0.7331,0.7231,0.5011,两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%,标本批内平均cv=4.42%,批间平均cv=3.42%;coulter maxm分析仪批内平均cv=5.70%,批间平均cv=17.02%;miller窥盘法批内平均cv=26.15%。结论:sysmex-r3500分析仪具有重复性好,简便,快捷的特点,尤其适合临床大批标本的快速测定。网织红细胞 miller;窥盘法;仪器法

Methods:RET_related parametersreticulocyte percentage(RET%, RET countRET#, immature RET fraction(IRF, mean RET volumeMRV, mean sphered cell volume(MSCV, high light scatter RET percentage and absolute valueHLR% and HLR#were determined using LH_750 automatic blood analyzer in patients with anemia.

网织红细胞相关参数测定在贫血初筛诊断中的意义&版权属于原作者所有!请勿将&网织红细胞相关参数测定在贫血初筛诊断中的意义用于商业用途!作者:黄华,陈林兴,尹俊,曾哲

Bone marrow polychromatic erythrocyte micronucleus rates of jimpy mice fed with different dosage olaquindox was detected by the way of toxicology,and the number of red blood cells,white blood cells and granulofilocyte was mensurated after 30 days.

给小鼠灌喂不同剂量喹乙醇,按毒理学方法检测小鼠骨髓嗜多染红细胞微核率,并在饲养30d后测定血液中红细胞、白细胞、网织红细胞数。

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