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RESULTS AND CONCLUSLON: A total of 2 of 6 autogenous bone grafting cases suffered from graft bone dislocation, 1 suffered from pseudoarthrosis formation, and 1 suffered from donated ilium. Six of 21 autogenous bone graft with cervical plate-screw fixation suffered from loss of intervertebral height, and 3 suffered from pain of donated ilium.

结果与结论:6例单纯植骨患者中2例发生植骨块脱出,1例发生融合节段假关节形成,1例发生髂骨供区疼痛;21例椎体间自体髂骨植骨内固定患者中6例发生椎间高度丢失,3例发生髂骨供区疼痛;65例椎体间钛网植骨融合内固定患者中11例发生内植物下沉,椎间高度丢失,1例发生内固定断裂。

Observed with transmission electronic microscope,ES-like cell have obvious nucleolus and a little heterochromatin,and many ribosomes in the cytoplasm with much endoplasmic reticulum and mitochondria. A part of cells appeared alveoli on the nuclear envelope,and chromatin was pycnosis and the .

在透射电镜下细胞核仁明显、核异染色质少;细胞质中核糖体较多,内质网、线粒体发达,部分细胞的细胞核膜上出现小泡,染色质边集,染色质形成凋亡小体。

A lot of organelles such as mitochondria, rough endoplasmic reticula and Golgi bodies were observed in fiber cell. Then, double karyotheca disappeared, and the organelles disintegrated. Multivesicular bodies appeared in fiber cytoplasm. With the further development of fiber, fiber wall underwent continual thickening with aging, and polylaminate structure gradually appeared. While the agglutinated nucleus, transfer vesicles, plasma membrane and plasmodesmata still presented.

研究发现,次生壁形成早期,细胞核具有双层核膜,染色质凝聚,可见大量的线粒体、粗面内质网和高尔基体等细胞器存在于纤维细胞中;随后,双层核膜消失,细胞器将逐渐解体,多泡体开始出现在纤维细胞的细胞质;随着年龄的增加,纤维细胞壁逐渐增厚,并出现多层结构现象,而运输小泡、细胞膜、胞间连丝和凝聚的染色质将持续存在。

The disintegration of tapetal cell was relayed, the inner wall of tapetal cell secreted fewer Ubish bodies.

花粉败育过程中,小孢子液泡膜和细胞质膜断裂破碎,细胞质解体,线粒体、质体、内质网等细胞器解体或退化,绒毡层持续不解体,并缺少乌氏体的分泌。

Methods 16 cases of thoracolumbar burst fractures with spinal cord or equina cauda injuries were treated with anterior subtotal vertebrectomy, Pyramesh with bone debris intervertebral implantation and Z-plate fixation.

16例胸腰椎爆裂性骨折伴脊髓或马尾损伤患者进行前路椎体次全切除减压,自体碎骨装入Pyramesh钛网后行椎体间植骨,加用Z-plate内固定。

Firstly, the acute cytotoxicity of profenofos on FG-9307 cells was studied, it was found that the cell growth rate was markedly reduced by profenofos at all the concentrations tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysosomes with engulfed organelles and numerous vacuoles in the cytoplasm.

丙溴磷对FG-9307的急性细胞毒性研究结果发现:1)在所有实验浓度下,细胞的生长速度均受到抑制。2)细胞的超微结构被丙溴磷所改变,最明显的特征则是线粒体损伤严重,嵴肿胀,部分溶解破裂;核膜肿胀,有溶解;细胞内的电子密度高的溶酶体增多;内质网的膜溶解,核糖体从粗面内质网上脱落下来,游离于胞质中。

However, the membrane of toxopalsma in liver tissues was stickled out and broken, vacuole lied in cytoplasma, mitochondrion and endoplasic reticulum were swollen or broken, and dense granules were disappearing TEM ×10000

肝组织内弓形虫随扁桃酸作用时间的延长,其受损程度明显加重。在治疗早期(24、72h)肝组织内虫体胞膜突出、破裂,胞质中出现空泡,线粒体和内质网肿胀或破裂,致密颗粒消失,虫体电子密度降低(图2B)。

Results Following orchiectomy 7d,the amount of CGRP-immunoreactive nerve fibers in the AP of the rat increased significantly, with the tortuous varicose fibers more densely distributed than that of NOR and the amount of varicosities increased significantly, while the thick fibers were more thicker than that of NOR,and many thin fibers were sent out from the thick fibers, and the varicosities were more larger.

结果 睾丸摘除7d后,大鼠垂体前叶CGRP免疫反应神经纤维发生了显著变化,表现为膨体型纤维较正常组稠密,分支增多,迂曲成片,盘绕成网,分布更加广泛,膨体数量显著增多;粗纤维较正常组粗,且发出许多迂曲盘绕的细纤维,膨体形态变大。t检验显示,OX组与NOR组垂体前叶内CGRP免疫反应神经纤维的平均面积密度差异显著,OX+V与NOR组差异显著。

ZSM two-drum pulse low net work of the screen, pulp pressure from the top of the screen to cut into the screen, the two fall in the middle drum sieve, sieve in the drum inside and outside of the pressure difference and the dual-rotor rotary-wing together, qualified fiber Through the inside and outside the drum screen after screen to the body at the bottom of the pool, and then by the good plasma discharge tube machine, the screen remained in the dark between the flow of rough slurry residue by the rotor and driven together to drum sieve under the tailings from the slot after the Slag discharge pipe.

ZSM双鼓式低脉冲网前筛工作时,纸浆从压力筛的上部切向进入筛体,流到两个筛鼓中间,在筛鼓内外的压力差及双旋翼转子的共同作用下,合格纤维通过内、外筛鼓后汇集到筛体底部,然后经良浆管排出机外,留在筛鼓之间的粗渣受到浆流和旋翼的推动,汇聚到筛鼓下的尾渣槽后从排渣管排出。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明,所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达;在缺乏AMBN情况下,与对照组相比,实验组牙胚在体外可以继续生长发育至钟状晚期,出现成釉细胞和成牙本质细胞的分化,成釉细胞可以分化成为分泌期型成釉细胞,胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体,缺乏溶酶体,表明对蛋白合成和脚的能力降低;实验组牙胚有牙尖形成和基质分泌,但牙尖形态异常,基质形成减少,牙尖周围基质最厚处为O.6卜m,明显薄于对照组的5.spin,基质中胶原纤维粗细不等,排列稀疏, 3 第四军医大学硕士学位论文未见钙化现象,充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程,影响胶原纤维和牙本质基质的合成,促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

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