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Result The detection limit of the two systems were 0.1fg, equal to 2.31×10^4 copies, which was 10^2-10^3 fold higher than conventional PCR. By the constructed detection systems, the TCK and Tilletia cariesTul could be distinguished. The teliospore and mycelium of TCK in the infected wheat plant tissue also could be identified accurately and rapidly.

建立的两套定量PCR检测体系的检测下限相当,可达到0.1fg,对应的拷贝数为2.31×10^4个,检测灵敏度比常规PCR高2~3个数量级,均可成功鉴别出TCK与小麦网腥黑穗病菌Tilletia caries (DC Tul, TCT,并可快速准确检测小麦矮腥黑穗菌冬孢子和检测罹病小麦植株体内的侵染菌丝体。

Experts have found that seaweed seedling net curtain on a very rare, it is difficult to see the Porphyra thallus intact.

专家们发现,网帘上紫菜小苗十分稀少,很难见到叶状体完好的紫菜。

Yang E,Korsmeyer SJ.Molecular thanatopsis:a discourse on the BCL-2 family and cell death.Blood,1996,88:386[3] Campos L,Rouanlt JP,Salido O,et al.High expression of BCL-2 protein in acute myeloid leukemia cells is associated with poor response to chemotherapy.

BCL-2是新一类癌基因——凋亡调控基因BCL-2家族的代表,其编码26kd的蛋白存在于线粒体、核膜及内质网,与同家族的其它成员组成不同比例的同、异二聚体而影响凋亡启动[6]。

Research result indicate both the Triangulated Irregular Network as a facial model and the Generalized Straight Triangular Prism Network as a volumetric model are suitable to coal seam 3D dynamic modelling.

研究表明不规则三角网(Triangulated Irregular Network,TIN)和广义直三棱柱网(Generalizedstraight Triangular Prism Network,GSTPN)分别适合煤层动态建模的表面模型和体模型。

Hyphae, loaded with CTC under pH 6. 8 condition and then grown in pH 8. 0 medium for a little while to destroy the apical acidification, could gave very diffuse fluorescence images of CTC membrame bound Ca〓 and vermiform fluorescence spots due to mitochondrias under pH 6. 8 no longer were clearly distinquished, which implied that besides mitochondrias, other cellular organelles with Ca〓 also were stained with CTC. Thus mitochondrias are not the only one intracellular Ca〓 storage, endoplasmic reticulums and Golgi bodies in the same zone may be also the Ca〓 storages. But the extreme apical zone under 2μm of the growing hyphal tip was still almost devoid of stain under pH 8. 0.The CTC fluorescence was concentrated in the subapic zone beyond about 2μm from the tip and then gradually became lower behind about 40μm from the tip. These results did not support the hypothesis which suggested that the cell wall vesicles in the extreme apical zone were intracellular Ca〓 storages.

将菌丝在pH6.8条件下负载CTC,再置于pH8.0培养介质中短暂生长一会儿以消散菌丝顶端的酸化区域,则CTC膜结合Ca〓呈现弥散的荧光影像,在pH 6.8培养条件下显示的蠕虫状的线粒体荧光斑点不再能够清晰辨认,说明除了线粒体之外,还有其它含Ca〓细胞器,也被CTC染色,线粒体并不是细胞内唯一Ca〓库,还可能包括内质网、高尔基体等细胞器,但菌丝最顶端2μm以前的细胞壁泡囊区域不能被染色,最大荧光强度仍位于菌丝顶端2μm以后区域,约45μm以后荧光变弱,实验结果不支持细胞壁泡囊为菌丝胞内Ca〓库的假设。

We found that during the first 24hours of treatment with PCE at the MSD , autophagy and vesiculation were induced in cultured GF .

在PCE 之浓度为 MSD 时培养约24小时后﹐人类牙龈纤维母细胞的颗粒内质网及高基氏体,几乎完全消失。

In hypothalamic median eminance of kidney Yang deficiency group, the bounds of ependymocytes were dim, distribution of their microvillus changed, the top of microvillus enlarged and became spherical, and holes occurred on the surface of the cells. In that of control group, the cell arranged in order and the cell bouns were clear, the microvillus were equal in thickness and in long, thin acerose thread wool shape. The surface ultrastructure of ependymocytes on hypothalamic median eminance in herb group was similar to that of control group.

肾阳虚大鼠垂体内分泌细胞和甲状腺细胞存在着不同程度的粗面内质网、高尔基体扩张,线粒体空化,细胞变形,核固缩等超微结构的损伤;中药组大鼠垂体超微结构与正常组类似,细胞形态完整,结构清晰,细胞器结构正常,含有各种大小不等、形态不一的分泌颗粒,但甲状腺细胞超微结构与肾阳虚组相比未见明显改善。

The polypores treated in the present list are in wide sense, including the poroid species in Polyporales, Hymenochaetales, Gloeophyllales, Trechisporales, Corticiales, Thelephorales and Russulales, and a few poroid genera of Agaricales, Atheliales, Cantharellales and Auriculariales (e.g. Dictyopanus, Favolaschia, Elmerina, Fistulina and Protomerulius).

本文中的多孔菌系指广义非褶菌目中具有孔状子实层体的种类,按照现代分类系统包括担子菌门中多孔菌目、锈革孔菌目、褐褶菌目、糙孢孔目、革菌目、糙孢革菌目和红菇目中孔状子实体的种类,还有伞菌目、阿太菌目、鸡油菌目和木耳目中个别属,如网孔菌属、胶孔菌属、榆孔菌属、牛排菌属和纵隔孔菌属等的种类。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚。

Amygdaloidal calcite aggregate and fine stockwork of calcite-prenite-chlorite are superimposed on the asphalt and carbon-rich ores.

在矿体中还见到葡萄石-绿泥石-方解石杏仁体和细网脉,与沥青团块抑或共生抑或稍晚于后者。

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I didn't watch TV last night, because it .

昨晚我没有看电视,因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。

I'm running my simile to an extreme.

我比喻得过头了。