缺氧的

Objective To observe the changes of the endocrine function and the ultrastructure of adrenocorticotrophic hormone cells in the rat＇ s pituitary gland treated with integripetal rhodiola herb under chronic hypoxia.

目的 观察红景天对慢性缺氧下大鼠腺垂体促肾上腺皮质激素细胞超微结构和内分泌功能的变化，探讨在慢性缺氧下红景天对ACTH细胞分泌功能的影响及其在机体缺氧应激反应中的作用。

The process of cerebral hypoxia was simulated by giving superfusion with ACSFOGD for 14 minutes in the control and test groups. The propofol test group was subdivided into P1, P5 and P15 groups to undergo superinfusion with ACSF containing 1, 5 and 15μmolL^(-1) propofol respectively.

缺氧组：脑片用缺氧无糖的人工脑脊液灌流14min，实施缺氧过程；药物组分为P1、P5和P15组：在ACSFOGD灌流液中分别含1，5和15μmolL^(-1)的丙泊酚。

Through researching the mechanism of biological nitrification and denitrification and actual project application of the technology and related literature materials, the obtained results are as follows:(1) Anoxybiosis is effective to the removal of COD; after the anoxic process, BOD/COD of the outflow increases, it shows that the degradation ability of wastewater is improved; anoxybiosis is insensitive when shock loading of inflow wastewater varies greatly;(2) It is demonstrated by the operation of inoculation and cultivating that biofilm takes two weeks to succeed in cultivating on packings and the removal ratios of NH3-N and COD get 50% and 70% respectively, when gas-water ratio is 6:1, HRT is 7.3 hour, water temperature is above 15°C;(3) It is demonstrated that gas-water ratio, hydraulic loading, HRT, temperature, pH etc are important factors which influence the removal effect of pollutants, when the gas-water ratio is 6:1, hydraulic loading is about 0.38kgNH3-N/m~3.d, water temperature ranges from 15°C to 27°C, pH ranges from 7.5 to 8.0, the BIOFOR removal effect of pollutants is the best;(4) The operation indicated that, this technology has strong ability of nitrification and denitrification; it has extremely vital significance for reducing the water body eutrophication;(5) It is confirmed the feasibility and the usability of preanoxic-BIOFOR process to treat L-lactic acid production wastewater. The experiment indicated that the treatment effect of this technology is very good and also shows that the technology has many advantages, such as small volume, high treatment efficiency, good effluent quality and strong endurance to load variation, this technology is a new and economical wastewater treatment process.

通过对生物脱氮机理的研究和该组合技术的实际工程应用，并结合有关文献资料，取得如下结论：(1)预缺氧池对L-乳酸生产废水中的COD去除具有一定的效果；废水中难降解的高分子物质经过缺氧工段分解为易于氧化降解的物质，浮状有机物在水解菌的作用下，将不溶性有机物水解为溶解性物质；预缺氧池具有较强的抗冲击负荷能力；(2)BIOFOR接种挂膜过程表明：HRT为7.3h、气水比为6：1、水温保持在15℃以上，经过半个月生物膜即以形成，NH_3-N和COD的去除率分别达到50％和70％左右；(3)研究表明：气水比、水力负荷、HRT、温度、pH等是影响污染物去除效率的重要因素，在气水比为6：1，NH_3-N容积负荷为0.38kgNH_3-N／m~3·d，温度在15℃-27℃，pH在7.5-8.0之间的条件下，BIOFOR对污染物的去除效率达到最佳；(4)运行表明，该组合技术具有较强的脱氮能力，对于减少水体富营养化具有十分重要的意义；(5)经过半年多实际工程的现场调试与运行，验证了利用预缺氧—BIOFOR组合工艺处理L—乳酸生产废水的可行性和实用性，得出该组合技术具有占地面积小，处理效果好，运行费用省，耐冲击负荷能力强等优点，是一项新型、经济的污水处理技术。

Results Both inhibitions of PKC with 6.8 mmolL^(-1) hypericin and PKA with 0.112 mmolL^(-1) KT5720 shifted the dose-response curve of 15-HETE induced pulmonary vasoconstriction to the right, and more powerful inhibition was observed with PKC inhibitor (P.01). Denuding endothelia decreased the response of PA ring to 15-HETE in both normoxic and hypoxic PA rings (P.05, P.01, respectively).

结果 用6.8 mmolL^(-1) hypericin阻断PKC途径可使15-HETE收缩缺氧大鼠肺动脉量效曲线右移(P.01)；用0.112 mmolL^(-1) KT5720阻断PKA途径使15-HETE收缩缺氧大鼠肺动脉量效曲线右移不明显；除去血管内皮细胞可降低对照组和缺氧组肺动脉血管环对15-HETE的反应性。

OLH or 8-bromo-cAMP were added to luteinized granulosa cells. Results indicated that, protein expression of HIF-1a, HIF-1b and VEGF had a better performance in hypoxia than in normoxic condition, but phospho-PKA, StAR and P450scc protein expression had weaker expression in the former condition than in the later one. As a result of decreasing secretion of progesterone the decreasing of phospho-PKA was suspected to have a role in the reduction of StAR and P450scc in the condition of hypoxia.

结果发现，黄体化粒性细胞於缺氧条件下其HIF-1a、HIF-1b与VEGF蛋白质表现量皆较正常氧高；同时，StAR及P450scc蛋白质表现量则降低，但3b-HSD不受缺氧影响；此外，藉由特异性抗体检测PKA讯息传递路径发现，PKA讯息传递路经於缺氧条件下磷酸化PKA（phospho-protein kinase A）蛋白质表现量下降，可能经此路径的调控造成StAR及P450scc蛋白质表现量下降，导致黄体化粒性细胞孕酮分泌量减少。

The machanism may be that 7-NI inhibits activation of nNOS and decreases the number of overproducing neuronal NO in hippocampal during anoxia,so the ability of hippicampal slices against anoxic injury is increased and neurotoxic role of neuronal NO during anoxia is definite.

作用机理可能是7-NI抑制nNOS活性降低缺氧后海马神经原性NO的过多形成，从而提高海马脑片抗缺氧损伤的能力，更加明确了缺氧早期神经原性NO的神经毒性作用。

Methods The chemical hypoxia was induced by incubation of U251 cells with cobalt chloride.

以氯化钴模拟缺氧，采用RT-PCR、免疫细胞化学方法分别检测缺氧诱导因子-1α和血管内皮生长因子mRNA和蛋白的表达，鸡胚绒毛尿囊膜模型检测缺氧时胶质瘤细胞血管生成情况。

The expressions of HIF-1αprotein were detected by Western blot, and we selected a properly cobalt chloride density and culture time as the most suitable culture condition.

1、用不同缺氧时间(0、12、24、48、72h)、不同浓度氯化钴(0、200、400、600、800μM)模拟缺氧培养食管鳞癌细胞Eca-109，以Western blot法检测细胞中HIF-1α蛋白的表达变化，选择可以引起HIF-1α蛋白表达增加的适当的氯化钴浓度和缺氧培养时间。

In this paper studies on the effect of the compositus on myocardial ischaemia and hypoxia are.

用低压缺氧和常压缺氧法，观察小鼠在缺氧条件下的存活率和存活时间。

During hypoxic exposure,the leakage of LDH was gradually increased , peaked at 8h ,then decreased. The same results were supported by electron telescope. At the same time, we used RT-PCR to observe the expression of HIF-1 a rnRNA.

在检测细胞损伤的同时，我们采用RT-PCR技术观察缺氧后HIF-1α mRNA的表达，发现在缺氧0h也有低表达，而后表现为时间依赖性，在缺氧4hmRNA的表达达到高峰，而后逐渐降低。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐