缺氧的

(1) cerebral ischemical reperfusion injury rats'limbs motor function is variable. Acupuncture could promote lims'functional recovery.(2) PCNA masc cells is visible in cerebral ischemical semidarkness region. There is cell regeneration phenomenon. Acupuncture could strengthen injury region's PCNA expression, could profit injury recovery and functional reconstruction.(3) In ischemia semidarkness region for the model group and acupuncture group, PCNA masc cells percentage of 14days group is lower than 7days group. Along with the recovery of injury, cell multiplication is weaken.(4) In cerebral ischemia semidarkness region, there is VEGF masc cells and regeneration phenomenon. Acupuncture could strengthen injury region's VEGF expression, could profit protection after injury and blood vessel regenerate.(5) In ischemia semidarkness region for the model group and acupuncture group, VEGF masc cells percentage of 14days group is lower than 7days group. Along with the recovery of semidarkness region, ischemia and anoxemia state is getting improved, and VEGF is reduce.(6) As there are PCNA and VEGF masc cells in brain injured region, we could conclude that, after brain ischemical reperfusion injury, there are blood vessel regeneration phenomenon. Acupuncture could promote blood vessel regeneration, recovery blood supply sufficiently and quickly, and promote the recovery of brain injury region.(7)The VEGF masc cells percentage of inhibitor group is lower than acupuncture group. It state that the effect of acupuncture promote VEGF is partly depend on the existing of eNOS.

实验结论：(1)脑缺血再灌注损伤后大鼠的肢体运动功能发生改变，针刺可以促进肢体功能恢复；(2)脑缺损伤区可见PCNA阳性细胞，存在细胞再生现象，针刺可以增强损伤区PCNA的表达，有利于损伤的修复和功能重建；(3)针刺组和模型组14d时缺血损伤区PCNA阳性细胞百分比低于7d组，随着损伤逐渐得到修复，细胞增殖现象减弱；(4)脑缺血损伤区可见VEGF阳性细胞，存在内皮型细胞再生现象，针刺可以增强损伤区VEGF的表达，有利于脑损伤后保护和缺血区血管再生；(5)针刺组和模型组14d时缺血损伤区VEGF阳性细胞百分比低于7d组，随着缺血损伤的修复，缺血缺氧状态得到改善，产生的VEGF减少；(6)由于脑损伤区同时出现PCNA阳性细胞和VEGF阳性细胞，前者是增殖细胞的标志，后者是促进血管再生的重要因子，可以推断，脑缺血再灌注损伤后脑内存在血管再生现象，针刺可以促进损伤区的血管再生，更迅速而充分的恢复损伤区的血供，促进脑损伤区的修复；(7)抑制剂针刺组脑损伤区VEGF阳性细胞百分比与针刺组相比有不同程度的降低，说明针刺促进缺血损伤区VEGF表达部分依赖eNOS的存在。

In order to research the relations of carbon sources to structure of PHAs, six kinds of PHAs sample were synthesized by activated sludge with six different carbon sources respectively in the aerobic-anaerobic-anoxic process. Their structures were determined by 〓HNMR、〓CNMR and GC-MS. When the carbon source was acetate, the unit composition of PHAs was 93. 91mol％ 3-hydroxybutanoic acid and 6. 09mol％ 3-hydroxypentanoic acid ; When the carbon source was propionate, the unit composition of PHAs was 28. 66mol％ HB, 63. 13mol％HV, 2. 55mol％ 2-methy-3-hydroxy-butanoic acid (2MHB) and 5. 66mol％ 2-methyl-3-hydroxypentanoic acid (2MHV); When the carbon source was 80％ butyrate and 20％ 1, 4-butanediol, the unit composition of PHAs was 65. 03mol％ HB, 28. 06mol％HV, 1. 91mol％2MHB, 2. 69mol％ 2MHV, 0.73mol％ 4-hydroxy butanoic acid (4HB), 0.78mol％ 4-methyl-3-hydroxy-pentanoic acid (4MHV), 0.80mol％ 3-hydroxyhexanoic acid ; When the carbon source was 20％ butyrate and 80％ 1, 4-butanediol, the unit composition of PHAs was 61. 39mol％ HB, 23. 01mol％HV, 4. 58mol％2MHB, 5. 97mol％ 2MHV, 0.91mol％ 4HB, 2. 37mol％ 4MHV, 1. 77mol％ HHx; When the carbon source was 60％ pentanoate and 40％glucose, the unit composition of PHAs was 41. 24mol％ HB, 58. 76mol％HV; When the carbon source was 60％ benzoate and 40％ glucose, the unit composition of PHAs was 65. 48mol％ HB and 34. 52mol％ HV.

为了研究不同碳源与产物PHAs结构的关系，采用好氧-厌氧-缺氧模式利用6种不同碳源培养活性污泥得到6种不同的PHAs样品，通过〓HNMR、〓CNMR和GC-MS谱图确定了这6种PHAs的单体成分和比例：以乙酸钠培养活性污泥得到的PHAs的单体组成是93.91mol％3-羟基丁酸和6.09mol％3-羟基戊酸；以丙酸钠培养活性污泥得到的PHAs的单体组成除28.66mol％HB、63.13mol％HV外，另外还有2.55mol％3-羟基2-甲基丁酸（2MHB）和5.66mol％3-羟基2-甲基戊酸（2MHV）；以80％丁酸钠和20％1，4-丁二醇混合溶液培养活性污泥得到的PHAs含有七种单体组成：65.03mol％HB，28.06mol％HV，1.91mol％2MHB，2.69mol％2MHV，0.73mol％4-HB（4-羟基丁酸），0.78mol％4MHV（3-羟基-4-甲基戊酸），0.80％HHx（3-羟基己酸）；以20％丁酸钠和80％1，4-丁二醇培养活性污泥所得到的PHAs含有与3#样品相同的七种单体，只是比例不同，分别是61.39mol％，23.01mol％，4.58mol％，5.97mol％，0.91mol％，2.37mol％，1.77mol％；以60％戊酸钠和40％葡萄糖培养活性污泥所得到的PHAs由HB和HV组成，比例是41.24 mol％∶58.76 mol％；以60％苯甲酸钠和40％葡萄糖培养活性污泥所得到的PHAs也由HB和HV组成，比例是65.48 mol％∶34.52 mol％。

The results showed that at the anaerobic phase, the PHB concentration in the cell and orthophosphate concentration of the anaerobic effluent obviously increased, the poly-P concentration in the cell decreased, and the cucci and bacilli with a size of 1.0~1.5 μm were dominant and formed grapy cellular cluster. At the anoxic phase, the PHB concentration and orthophosphate concentration decreased significantly, the amount of poly-P increased, and the elliptical cucci of 0.5~1 μm and spheral bacilli of 1.0~1.5 μm were dominant. At the aerobic phase, the PHB and orthophosphate concentration almost reduced to zero, the poly-P increased significantly, cucci appeared single or in pair, and the contour of sphere was blur and almost disappeared.

试验结果表明，在厌氧阶段，微生物细胞内PHB的量大幅提高，上清液中磷酸盐的含量上升，聚磷酸盐含量明显下降，粒径为1～1.5 μm、呈球状和棒状的菌群构成的葡萄状细胞簇占居优势；在缺氧阶段，微生物细胞内PHB的量下降，上清液中磷酸盐含量下降，聚磷酸盐含量上升，粒径为0.5～1 μm的椭球菌与1.0～1.5 μm的球杆菌占优势；在好氧阶段，微生物细胞内PHB的量较低或接近零，上清液中磷酸盐的含量接近零，聚磷酸盐含量明显上升，此时粒径1.0～1.5 μm的球菌以单个或成对出现，球菌不再饱满，呈现接近消失的状态。

According to the above study result, the diversity of microorganisms population in the SUFR system is complex and the bio-community formed in the SUFR system is stable. The close relations between abundance of bacteria and nutrients were found. The correlation between heterotrophic bacteria and COD was 0.949. The correlation between organic phosphate bacteria and TP was 0.815. The correlation between nitrosobacteria and NH3-N was 0.909. The correlation between disnitrifier bacteria and TN was 0.653. These inneglectable great factors effecting on phosphorus-uptake and phosphorus-release of phosphorus accumulating organisms are the influent COD、the concentration of DO、the sludge retention time、the temperature. The diversity of organic phosphate bacteria population in the SUFR reactor was complex and there is no phosphate bacteria taking the absolute superiority in quantity. The near-native pure culture method powerfully complement the traditional pure culture technique and enrich culture technique. The sludgy bioactivity is very good in whole SUFR reactor. By the quality of being biochemistry, the anaerobic phase is higher than anoxic phase and the anoxic phase is higher than aerobic phase of SUFR.

通过以上试验内容研究发现，SUFR 系统中的微型动物种群较多，微生物生态系统稳定；系统中微生物的数量与营养盐的含量密切相关，其中总异养菌与COD的相关系数r 为0.949，有机磷细菌与TP 的相关系数r 为0.815，亚硝化菌与NH4+-N的相关系数r 为0.909，反硝化菌的数量和TN 的相关系数r 为0.653；污水生物除磷工艺中进水COD 的浓度、DO 浓度、泥龄的长短、温度都是影响聚磷菌释磷及吸磷效果的不可忽视的因素；SUFR 系统中的磷细菌呈现种群多样化的趋势，没有占绝对优势数量的磷细菌；微孔滤膜近自然培养法是对传统纯培养技术和富集培养技术的有力补充；整个SUFR 反应器系统中污泥的生物活性很好，就可生化性来讲，厌氧反应器大于缺氧反应器大于好氧反应器；按照动力学方程式求出的细胞生长动力学特征值和SUFR 脱氮除磷系统工艺的实测值结果基本吻合。

The spiracles are bigger and more near dorsal line than all the others. Their trachea branches are taper and abundant and form short tracheoles. This gave the tufted form to the trachea, which appeared shrubby. The trachea branches that arranged in other segments gave them a tree-like look. They do not directly supply oxygen for tissues, but they suspend in the hemolymph and attach to the muscle of the heart, so that they can move in constant with the contraction of the heart muscle.②The 8th segment tracheal tufts have thinner cuticle and basal lamina than that of the other trachea, and have much pad cuticle, which is appropriate for them to move, and their basal lamina have small pores, which are favour of the permeation of gas.③Injecting the methylene blue into the larvae to label hemolymph, we observed that the hemolymph flow into the heart through the 8th segment tracheal tufts.④Normally these tracheal tufts have all kinds of hemocytes, which are abundant in the tufts. A large number of hemocytes released from tissues attach to the tufts by changing their structure when anoxic stress happen.

气管分支细短而丰富，形成气管簇，呈灌木丛状（其它各节气管则呈粗树干状分布），它不是直接给组织供氧，而是悬浮在血液中，与心肌相连接，随着心肌的收缩而不断运动；②气管簇的内膜及底膜都比其它各节的薄，且管壁内膜具有更多的适合运动的垫状表皮，底膜还有小孔结构，这有利于气体的通透；③向体内注射蓝色的亚甲基蓝标记血液发现，血液流动方向是经该气管簇后而流回心脏；④正常情况下，气管簇处有各种各样的血细胞聚集，当缺氧胁迫时有大量的血细胞从组织中释放出来，通过变形而紧贴气管簇，有利于气体的交换；⑤给体内注射无色的亚甲基蓝发现气管簇比其它各节更易变蓝，说明该处具有更高的获氧能力。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。