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To explore the changes of endogenous activin A in neonates with asphyxia and the relation between ACT A and hypoxic?ischemic encephalopathy.

目的 研究内源性激活素A的水平变化及其与新生儿窒息后缺氧缺血性脑病的发生、严重程度以及预后的相关性。

Neuroglobin, a recently identified monomer globin in nerve system, is highly affinitive with oxygen similar to myoglobin. After the coding region of rat NGB gene was sub cloned into the prokaryotic expression vector pGEX 4T 2, the recombinant vector was introduced into E. coli BL21 (DE3) cells for efficient expression.

氧是人类及许多生物赖以生存的重要条件,严重缺氧和低氧可危及人体生命。2 0 0 0年 9月,德国Burmester等人首次报道了人和小鼠脑内存在特异的功能上类似于肌红蛋白的携氧蛋白———脑红蛋白(neuroglobin ,NGB) [1 ] 。

Results: Neonatal rats in hypoxic-ischemicgroup behaved restlessness, cyanosis, deep and rapid breath, astasia, lethargy, irritation and spasm; There were local pyknosis, fragmentation, dissymmetry, blur or disappearance of nucleoli, raritas of ground substance in left cerebral cortex; but there was no statistic significance in chinese traditional medicine group and sham group. The expression of HIF-1α mRNA in brain tissue of experimental group and chinese traditional medicine group were higher than that in sham group, especially chinese traditional medicine group at 3d; HIF-1α positive cells were found in cerebral cortex and hippocampus. There were more HIF-1α positive cells in experimental group and chinese traditional medicine group and mainly in vascular endothelial cells.

结果 缺氧缺血后大鼠相继表现为烦躁不安、全身发给、呼吸加深加快、站立不稳、嗜睡、激惹或间断发作的痉挛和抽搐;HE染色显示实验组大鼠左侧大脑皮层出现局灶性神经元核固缩,核碎裂,核仁偏位,不清或消失,基质疏松;中药组大鼠脑组织结构基本接近假手术组,无明显水肿和坏死表现;实验组和中药组大鼠脑组织HIF-1α mRNA表达较假手术组明显增加,尤其是中药组实验第3日者;免疫组化显示实验组和中药组各时间点大鼠大脑皮层和海马区均可见不同程度的HIF-1α表达,明显高于假手术组,阳性表达主要在血管内皮细胞,海马和皮层的锥体细胞亦有HIF-1α阳性细胞分布。

It is concluded that the negative chronotropic effect and the decrease in APD induced by hypoxia may be attributed to the activation of K〓 channel and the release of endogenous ET.

上述结果表明,缺氧所致起搏细胞的负性频率作用和APD缩短,与〓通道的激活和内源性ET-1的释放有关。

It is conclud ed that the negative chronotropic effect and the decrease in APD induced by hypoxia may be attributed to the activation of K_channel and the release of endogenous ET.

上述结果表明,缺氧所致起搏细胞的负性频率作用和APD缩短,与KATP通道的激活和内源性ET-1的释放有关。

1H-MRS can detect brain metabolic changes in patients with cyanosed congenital heart disease in vivo noninvasively and can detect the metabolism disorder of the energy and amino acid,so the pathophysiology of this disease can be understood.

磁共振质子波谱能无创性地观察到紫绀型先天性心脏病患儿脑代谢异常的部分产物,可揭示慢性缺氧对脑组织代谢的影响及脑损害的病理生理机制。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability, while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or P .01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or P .01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05, P 。01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05, P 。01);4种单体对过氧化氢损伤无显著的保护作用。

Result: At most up to 500 mg·L-1, tetrahydropalmatine showed no sinificant effect on the cell viability; while dehydrocorydaline, berberine and palmatine significantly decreased the cell viability, exceeding 6.3, 0.6 and 6.3 mg·L-1, respectively( P .05 or 0.01). Tetrahydropalmatine, dehydrocorydaline, berberine and palmatine significantly inhibited LDH leakage induced by anoxia-reoxia injury, at concentrations of 50-100, 1.25-5, 4 and 30 mg·L-1, respectively( P .05 or 0.01). None of the four compounds showed significant effect on the hydrogen peroxide injury.

结果:多至500 mg·L-1的延胡索乙素对细胞活力无任何影响,脱氢紫堇碱、黄连素、巴马汀分别在质量浓度大于6.3, 0.6,6.3 mg·L-1时,显著降低细胞活力( P 。05或0.01);延胡索乙素、脱氢紫堇碱、黄连素和巴马汀分别在质量浓度为50~100, 1.25~5, 4,30 mg·L-1时,显著抑制缺氧复氧导致的LDH漏出( P 。05或0.01);4种单体对过氧化氢损伤无显著的作用。

In hypoxia/reoxygenation model(Na2S2O4), cell survival was enhanced and LDH in culture medium was reduced(P.05, P.01) after treated with 60, 30μgmL^(-1) Sanguis Draconis flavones. Enhanced cell survival and reduced LDH in culture medium(P.05, P.01) also observed in cytotoxicity model induced by H2O2 after incubated with 60, 30, 15μgmL^(-1) Sanguis Draconis flavones.

在缺氧/复氧(Na2S2O4)模型中,60、30μgmL^(-1)龙血竭总黄酮能显著降低培养液中的LDH浓度,增强细胞活力(P.05, P.01)。60、30、15μgmL^(-1)龙血竭总黄酮能抑制H2O2引起的损伤,降低培养液中LDH的浓度,增强受损细胞活力(P.05, P.01)。

The result showed "TDR" can prolong the average and highest 1ife-span of the eumenid; it also reduced the serum; liver and brain tissue's Lpo from the white rats 'obviously, at the same time, it build up the immunity, anti-stress, endures anoxia. To do so, the dosage of the "TDR" is crucial enableto achieve the clinical effectiveness.

结果表明:天地人口服液能延长果蝇平均寿命和最高寿命,能明显地降低大白鼠的血清、肝、脑组织的LPO,同时提高血清SOD的活性,并且有增强小鼠免疫功能、抗疲劳、耐缺氧,其药效与剂量有关。

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