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Most patients' death arise from the metastatic spread of primary tumors There is an urgent need for the finding of novel anti-tumor and anti–metastatic drugs to improve the quality of life and survival rate of patents Justicia procumbens is an herb and used to reduce fever inflammation and canker in Taiwan We've detected that 0 3 μM JA could effectively inhibit the proliferation of oral cancer cells OC-2 and HSC3 but not normal oral keratinocytes in MTS assay JA-treated OC2 underwent apoptosis as suggested by increased sub-G1 DNA contents condensed nuclear morphologh and DNA fragmentiation The migration activity of OC-2 was also significantly reduced by treatment with low concentration of JA The expression of ?

大部分的口腔癌病患死於原位癌的转移和扩散，寻找有效抗癌和抗转移的药物疗法来增进病患的生活品质和存活率，对治疗口腔癌而言非常重要。爵床草是台湾民间常用草药，常被用来消炎止热和治疗口疮、乳瘤。我们利用MTS分析，发现处以0 3 μM的JA 48小时，即可有效抑制口腔癌细胞OC-2和HSC3的增生，但不影响正常口腔上皮细胞生长。OC-2经JA处理后，其sub-G1的DNA含量增加，并可观察到DNA固缩和梯度断裂的现象，说明JA可使OC-2走向细胞凋亡。除此之外，0 3 μM的JA亦可有效降低OC-2的迁移能力。

Results Characteristic changes of HNE1 apoptosis were significantly observed after PDT, including early occurrence of cell condensation, karyopyknosis and karyorrhesis.

PDT 12h后，实验组HNE1细胞开始变形皱缩，细胞界限模糊，细胞透光度和立体感较差，治疗 2 4～ 4 8h后细胞发生凋亡。结论体外光动力学治疗对HNE1具有明显的杀伤效应并诱导HNE1细胞凋亡。

Result 1 Magnetic nanoparticles, magnetic nanoparticles modified with antisense oligodeoxynucleotide of human telomerase reverse transcriptase induced HL-60 tumor cells to apoptosis, we could see typical morphologic change of apoptosis cells: karyopyknosis, chromation"s condensing and aggregation in nuclear, forming crescent-shaped or annulus structures to lean on edge of cell nucleus"s membrane and posing apoptosis body by Atomic Force Microscope, Fluorescence microscope, transmission electron Microscope 2 There was a significant difference compared with control group(p.01), inhibition ratio had significant positive correlation with medication dosage and time ;during 0.8-8μM dosage amplitude, inhibition ratio accrescenced by dosages increasing. However, the inhibition ratio would decrease when dosage over 8-80μM.

结果 1 磁性纳米粒子、修饰有端粒酶反义寡核苷酸的磁性纳米粒子诱导HL-60细胞发生凋亡，原子力显微镜、光学显微镜、荧光显微镜和透射电镜下均观察到HL-60细胞呈现典型的凋亡细胞的形态变化：细胞核固缩，核内染色质浓缩、凝聚、形成新月形或环状结构紧靠在细胞核膜边缘，并形成凋亡小体。2 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子对HL-60肿瘤细胞的生长和增殖有明显的抑制作用，与对照组相比有显著性差异(p＜0.01)，在剂量为0.8-8μmol／L范围内，抑制率随剂量的增加而增加，当剂量超过8μmol／L时，抑制率反而下降；3 磁性纳米粒子、修饰有端粒酶反义寡聚脱氧核苷酸的磁性纳米粒子可增强p53基因的表达活性，引起DNA降解损伤，反向调节细胞周期活动，促使细胞从G0期进入G1期，抑制肿瘤细胞的生长。4 修饰有端粒酶反义寡聚脱氧核苷酸的量子点能通过内吞作用进入HL-60肿瘤细胞的细胞核，可以在细胞内进行定位和促进HL-60肿瘤细胞的凋亡。

The proliferating cells were mainly distributed in the peri-chondrium and the peripheral zone of tracheal cartilage. The senescent chondrocytes were distributed in the central zone whereapoptotic morphological characteristics obviously appeared, such as pyknosis, karyorrhexis.

结果：软骨细胞呈现多种方式的无丝分裂像；气管软骨膜与软骨周围带多见细胞增生；气管软骨中央带多见衰老的软骨细胞，并见明显核固缩、核碎裂等细胞凋亡形态学特征。

A 30 min exposure to peroxynitrite (10〓μmol/L) followed by incubation in fresh medium induced apoptosis in cultured cerebellar granule cells, which was confirmed by DNA laddering, chromatin condensation, nuclei fragmentation, and the formation of apoptotic bodies.

原代小脑颗粒细胞经10μmol/L过氧化亚硝基处理30min，并继续培养一定时间后逐渐发生细胞凋亡，主要表现为DNA链断裂、染色质凝缩、核破裂、形成凋亡小体。

Morphologically, the macrophages treated with NO and O-LDL presented shrinkage, nucleus fragmentation, and cytoplasm condensation; DNA ladder was visualized by agarose gel electrophoresis; Characteristic apoptosis peak of macrophages was showed by flow cytometry; Ultrastructure, The chromatin condensed and marginated to form dense chromatin fragmentation; The cytoplasm concentrated and vacuolized; The lysosomes and mitochondria of apoptosis macrophages increased clearly.

电镜观察超微结构变化：染色质固缩边聚，形成致密、均质的染色质块，胞质浓缩、电子密度升高并空泡化；凋亡巨噬细胞溶酶体、线粒体增多；细胞突起缩短减少，但巨噬细胞特有的板状伪足不消失。

Pharmacological and biochemical study on the effects of selective phosphodiesterase inhibitors on human term myometrium［J］.

结果显示：高浓度的MS23可减小大鼠胸主动脉环的静息张力，并能抑制KCl和NE引起的缩血管作用。

Results Infected by this peptide, cell viability decreased 28.9%. Under light microscope, cells were shrinked and rounded, many cells were divorced from plate wall, some neuraxon shortened and broke. Apoptotic cells which nucleolus shrinked and rounded could be coloured orange by fluorescent colouration. Under electron microscope, chromatin gathered along the inside of the nuclear membrane, vacuole bodies appeared. Apoptotic peak was detected by flow cytometry and the ladder band appeared in DNA electrophoresis.

结果：细胞接触肽段后存活率下降28.9％；光镜下可见细胞贴壁不良，胞体缩小，细胞突起断裂缩短；荧光染色可见细胞突起缩短、胞核固缩、胞质染成橘红色的凋亡细胞；电镜下可见胞质中出现空泡样结构，细胞染色质浓集于核膜内侧并裂解成碎块状；流式细胞仪检测细胞出现亚二倍体峰，DNA电泳出现梯状带。

Through condensing o-aminobenzoic acid, ethyl oxalyl chloride and ethylenediamine(or 1,2-propylenediamine), two new ligands N-benzoato-N′-(2- aminoethyl)oxamido(H3oxbe) and N-benzoato-N-(2-amino-2-methylethyl)oxamido(H3oxbt) have been synthesized. Their mononuclear complexes Na·1.5H2O and Na·1.5H2O have also been obtained.

2以邻氨基苯甲酸、草酸乙酯酰氯和乙二胺(或1，2-丙二胺)缩合制得了两种具有不对称草酰胺结构配体N-(2-苯甲酰基)-N&-(2-氨乙基)草酰胺(H3oxbe)和N-(2-苯甲酰基)-N&-(2-氨丙基)草酰胺(H3oxbt)，并得到了它们的单核配合物：Na·1.5H2O和Na·1.5H2O。

Results The model contains 45 153 elements and 27763nodes. It could be arbitrarily rotated, reduced and added, perspectived, dissected, and be revised and adjusted according to different demands, and then simulated the different situations of alveolar bone absorption and restoration with linked crown asked by clinical need, inspected the stress response of the mandible anterior teeth with different conditions of restrictions or loaded stress.

结果：模型共有单元45 153个，节点27 763个，可以根据要求任意旋转、缩放、透视、剖开，进行多种方式观察；并可以按照不同研究目的，对模型进行修改和调整，来模拟不同程度牙槽骨吸收、联冠修复以及各种约束和加载条件下前牙的力学反应。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。