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缓冲物

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In an acetate buffer medium of pH 6.6, a colored complex was formed between pentavalent vanadium ion and pyrogallic acid.

于pH 6.6的乙酸盐缓冲溶液中,五价钒离子与焦性没食子酸反应生成有色络合物。

The primers of sheep SRY gene designed in this study are very specific beside cattle, the DNA of other animal would not affect the sexing result.

特异性检测结果表明:SRY基因引物只对羊、牛雄性特异,其他动物雄性DNA及淋巴细胞缓冲液均不影响检测结果。

The cytoplasmic extract was obtained through the lysis buffer containing high concentration of salt, eradicator and glycerol.

用含有高盐、高去污剂、高甘油的细胞裂解缓冲液制得了细胞抽提物。

If it is blocking the Sun, acting as a buffer, its S Pole absorbing magnetons so the Sun's surface becomes quiet, then sunspots will seem to disappear and other emissions from the Sun may be deflected by the Planet X, whose influence is over an immense areas beyond merely the planet itself.

如果它阻碍了太阳,充当了一个缓冲,它的南极吸收太阳的磁子,因而太阳的表面变得很平静,那么太阳黑子看起来消失了,并且来自太阳的其它放射物也会被行星 X偏移,它在一片巨大的区域造成了影响,不仅仅是对行星本身形成影响。

Mn oxidates dimethyl sulfoxide to dimethyl sulfone (DMSO2) at pH=6.0 of Tris-HCl buffer solution, Mn could react with dimethyl sulfone to form an ion association complex, so that the system's scattering intensity of RLS is enhanced.

在pH=6.0的Tris-HCl缓冲溶液中,锰氧化二甲基亚砜成为二甲基砜(DMSO2),生成的Mn(上标 2+)与DMSO2结合形成离子缔合物,使体系的共振光散射急聚增强。

The optimum condition of the ELISA are set as follows: 1ug/mL of NFLX -HSA(norfloxacin -human serum albumin) conjugation used as a coated antigen dissolved in carbonate buffer solution(CBS,pH 9.6,0.05mol/L), 0.5 % glutin in PBS(pH7.4,0.01mol/L) as a blocking agent post-coating, horse radish heroxidase-labeled sheep anti-mouse IgG(HRP-anti IgG) diluted by PBS(pH 7.4,0.1mol/L) in 1:1000 as a secondary antibody, and TMB (3,3",5,5"-tetramethyl benzidine dihydrochloride) as a sbustrate.

成功建立了检测抗诺氟沙星抗体的间接ELISA方法,其主要条件如下:pH9.6、0.005mol/L的碳酸盐缓冲溶液为包被液,1μg/mL的连接抗原NFLX-BSA(诺氟沙星-牛血清白蛋白)为包被抗原,1%明胶为封闭液,1:1000稀释的HRP酶标记的羊抗小鼠抗体作为二抗,TMB(3,3,5,5-四甲基联苯胺)作为底物。

Column: Agilent C18; mobile phase 10mol.l-1 monobasic Acetate buffer, 20 molL-1triethylamine solution adjusted to pH5.5 with Acetic acid -acetonitrile ,gradient elution, flow rate: 0.6 mLmin-1; Results:The Traditional Chinese Medicines contained Acarbose, Phenformin and Glibenclamide, and can be separated completely.

色谱柱:Agilent ZORBAX ODS C18柱(5μm,4.6×250mm, Agilent);流动相:醋酸盐缓冲液(10molL-1醋酸胺,20 molL-1三乙胺,用醋酸调节pH5.5)与乙腈,梯度洗脱,流速:0.6 mLmin-1,色谱检测波长234nm,格列吡嗪为内标物进行定量分析。

Column: Agilent C18; mobile phase 10mol.l-1 monobasic Acetate buffer, 20 mol·L-1triethylamine solution adjusted to pH5.5 with Acetic acid -acetonitrile ,gradient elution, flow rate: 0.6 mL·min-1; Results:The Traditional Chinese Medicines contained Acarbose, Phenformin and Glibenclamide, and can be separated completely.

色谱柱:Agilent ZORBAX ODS C18柱(5μm,4.6×250mm, Agilent);流动相:醋酸盐缓冲液(10mol·L-1醋酸胺,20 mol·L-1三乙胺,用醋酸调节pH5.5)与乙腈,梯度洗脱,流速:0.6 mL·min-1,色谱检测波长234nm,格列吡嗪为内标物进行定量分析。

Just mix your template with a buffer and some primers,Nucleotides and polymerases, too.

你只要把你的模板DNA(就是带有目的DNA的DNA跟缓冲液混匀了,当然也要加一些引物,核苷和聚合酶。

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