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METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤,将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮,再置入曲拉通X-100溶液使真皮细胞完全脱净,磷酸盐缓冲液反复清洗,即为脱细胞真皮基质,4 ℃保存备用。

Cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤,将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮,再置入曲拉通X-100溶液使真皮细胞完全脱净,磷酸盐缓冲液反复清洗,即为脱细胞真皮基质,4 ℃保存备用。

In this paper,we describe the preparation of YBCO coated conductor by IBAD technology route and the latest research results in the world.

采用X射线衍射仪分析YSZ缓冲层的取向;利用原子力显微镜和扫描电镜观察其表面形貌。

Low γ ray with 59.6keV energy acts as excitation for rare earth inaqueous and organic phase samples in separation processes by solvent extraction. As aresult, characteristic K series X-ray fluorescence of rare earths emitted. X-ray wasdetected by high purity planar Ge after transmitting Be window. The signals wereamplified by pre-amplifier or main amplifier, and the amulated signals are converted todigital ones.

对溶剂萃取法生产稀土流程中的有机相和水相试样,采用放射性核素〓的59.6keV单色能量激发稀土元素,得到特征K系X射线荧光,透过Be窗经高纯平面锗半导体探测器检测其强度,再经放大器、模数转换和多道缓冲存诸记录试样的能谱。

At the same time the buffer layer is incrassated, andthe log-law region moves upwards.

实验发现,具有减阻效果的涂料使近壁区流向平均速度增加,缓冲层增厚,对数律区上移,产生减阻效果。

Convolutional Interleaver because it is so final results for the numerical value is that the previous data on the number of packet buffer.

因为是卷积交织所以最后结果中出现为的数值表示该值是前一包缓冲数据的数。

Gene lies in nucleic acid sequence of endogenesis transcriptino alternation district between 16 Ss-23 S rDNA, reagent box includes 10 x PCR buffer, 10 X dNTP, 10 x introductor Cff A1/introductor CffA2 and Tag enzyme.

基因位于16S-23S rDNA间的内源转录间隔区的核酸序列,试剂盒包括10×PCR缓冲液、10×dNTP、10×引物CffA1/引物CffA2和Tag酶。

The results indicates that enzyme combination diluent is suited for the production of HCV antibody enzyme linked immunosorbent diagnostic reagents,if the enzyme combination diluent regards PBST as buffer liquid and BSA and Casein irrelated albumen as stability .

结论以PBST为缓冲液,以BSA和C as ein无关蛋白作稳定剂组成酶结合物稀释液适用于HCV抗体酶联免疫诊断试剂的生产。

After loading the sample, analysis started with a methanol gradient in buffer A from 65% to 80% for 20 min. and then with isocratic elution of 100% methanol for 10 min.

装船后,分析了样本用甲醇梯度的缓冲液,由65%的80%然后用20术:目标可以隐形等静洗脱100%甲醇 10分钟。

Here at the isoelectric point, buffering is minimal.

这是它的等电点,缓冲能力达到最小值。

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