绿原酸

The suitable detection wavelength was selected through qualitative determination of wave spectrum. With 95% ethanol as the reference solution, the standard curve was drawed with a series of different concn. of standard solution of chlorogenic acid, and the Absorbance of extracting solution was determined and the chlorogenic acid content in filtrate was calculated.

波谱定性检验选择适宜的测定波长，以95%乙醇为参比溶液，用配制好的一系列不同浓度的绿原酸标准溶液绘制标准曲线，测定提取液的吸光度，计算滤液中绿原酸的含量。

The results indicated that with the increasing of storage time, the leaves etiolated, contents of crude-fiber increased, while vitamin C, chlorogenic acid decreased, nitrate contents up to the maximum at the 3th day after storage , then decreased gradually. Stored at 2℃, the contents of chlorophyll, vitamin C, chlorogenic acid were higher, nitrate and crude-fiber contents were lower. The lower of membrane permeability and MDA content, the higher activity of SOD significantly delayed senescence process and prolonged storage life of Taraxacum mongolicum.

结果表明：采后蒲公英随着贮藏天数的增加，叶片逐渐黄化，粗纤维的含量升高，维生素C和绿原酸含量下降，硝酸盐含量先升高后降低；2℃下贮藏能够使采后蒲公英具有较高的叶绿素、维生素C、绿原酸，较低的硝酸盐和粗纤维含量，较低的细胞膜渗透率、MDA含量和较高的SOD活性，显著地延缓其衰老进程，延长贮藏寿命。

After the studies on the fruit of Viburnum sargentii Koehne, it is showed that it has the effect to the cough and it has no toxicity; The active part is the acidity part. Through the GC-MS, we obtain 23 compounds from the volatile oil. By HPLC analytical method, the content of chlorogenic acid is 0.82%.

本文通过对小鼠氨水引咳实验和急性毒性实验，证明了本品的止咳作用，并且没有毒性；在有效部位的筛选中，确定其有效部位为NaOH洗脱部分；在挥发油的成分测定中，共分离出32个峰，鉴定出23个化合物，占挥发油总量的95％以上；在绿原酸的含量测定中，测出其绿原酸含量为0.82％。

Spraying with chitosan of different concentration significantly increased the content of chlorophyll ,anthoxanthin ,chlorogentic acid, polyphenal ,soluble protein and total soluble sugar of Broccoli flowers. 40 mg L-l chitosan showed the most remarkable effet,compared to control, the content of chlorophyll ,anthoxanthin ,chlorogentic acid, total soluble sugar and soluble protein of treated Broccoli flowers increased by 64.4%,282.5%,35.6%,16.2% 22.7% respectively.PAL and PPO are key enzymes in synthesize of phenolics.No relation was found between phenolics synthesize and PAL activity in Broccoli flowers,but it showed positive relation between phenolics synthesize and PPO activity.

研究表明，本研究中，不同浓度几丁聚糖喷施对青花菜花球的叶绿素、类黄酮、绿原酸、多酚总量、可溶性蛋白及可溶性总糖的含浙江大学硕卜学位论文(2004)量有显著增加，其中以浓度为40mgL一'影响最为显著，花球中的叶绿素、类黄酮、绿原酸、可溶性总糖、可溶性蛋白分别较对照提高64.4%、282.5%、35.6%、 16.2%、22.7%，苯丙氨酸解氨酶、多酚氧化酶是酚类物质合成中关键酶，花球内酚类物质合成与苯丙氨酸解氨酶的活性没有一定的相关性，而与多酚氧化酶的活性呈正相关。

It aimed to offer reference for the development and utilization of chlorogenic acid.

简要介绍了绿原酸的理化性质和生物活性，综述了近年来绿原酸几种提取工艺的研究进展，并对其作了简要比较分析，着重讨论了绿原酸提取工艺中存在的问题，探讨了其今后的发展方向，旨在为绿原酸的开发利用提供参考。

Results: Nine compounds were obtained from the ethyl acetate fraction of C. speciosa and were identified as methyl chlorogenate, 5-O-cafeoyl quinic acid butyl ester, quinic acid butyl ester, 5-hydroxymethyl-furan-2-carbaldehyde, triacontanoic acid,-epicatichin, 7,8-dihydroxy-coumanrin, protocatechuic acid ethyl ester, and protocatechuic acid.

结果：从皱皮木瓜的乙酸乙酯萃取部位分离鉴定了9种化合物，分别为绿原酸甲酯，5-O-咖啡酰基－奎宁酸丁酯，奎宁酸丁酯，5-羟甲基－2-糠醛，三十烷酸，-表儿茶素，7，8-二羟基香豆素，3，4-二羟基苯甲酸乙酯，原儿茶酸。

Eight phenolic acids in Satsuma mandarin during maturity were determined by high-performance liquid chromatography coupled with photodiode array detector in this study, which were:protocatechuic, p-hydroxybenzoic, vanillic, caffeic, p-coumaric, ferulic, sinapic and chlorogenic.

采用HPLC-PDA测定了不同成熟期温州蜜桔中八种酚酸的含量，即绿原酸、咖啡酸、对香豆酸、阿魏酸、芥子酸、原儿茶酸、对羟基苯甲酸和香草酸。

Optimization of the extracting method of Chlorogenic Acid from Flos Lonicerae was studied by three different methods of ultrasonic extraction, sorbitic extraction, and improved method of sorbitic extraction.

采用超声波法、索氏法、改良索氏法3种方法分别从金银花中提取绿原酸，用紫外光谱法和高效液相色谱法对其进行定性定量分析。同时用金银花乙醇提取物及纯化的绿原酸对乳房炎主要致病菌进行抑菌试验。

By comparison of eight micro adsorption, A resin with high adsorption rate and eluent rate was used to adsorb chlorogenic acid from extraction solution. The adsorption parameters are: the content of chlorogenic acid is 0.204 mg per mL adsorption solution, the acidity of adsorption solution is 4, the adsorption velocity of flow is 3 mL per min, the eluent is 30% ethanol, the eluting speed is 1.5 mL per min and the eluents volume is 4 times of resin volume. In the optimal adsorption condition, the final yield of CHA is 5.06%. the content of chlorogenic acid is 78.26% in dry productin.

通过对比8种大孔吸附树脂对绿原酸的吸附效果，选用对绿原酸吸附解吸性能均较好的树脂对绿原酸吸附，吸附参数如下：吸附液绿原酸的浓度为0.204mg／mL、吸附液pH值为4、流速为3mL／min，解吸参数如下：解吸剂为30％乙醇、解吸剂流速为1.5mL／min、解吸剂使用量为4倍树脂体积，在此条件下，绿原酸得率为5.06％，产物中绿原酸含量为78.26％。

In this research, liposoluble chlorogenic laurate was synthesized by the acylation with lauroy chloride in the existence of alkali catalyst in non-water phase, and yellowish CGL powders with a yield of 81.24% were obtained under the optimal synthesis conditions determined via single-factor tests and orthogonal experiments, that is, carrying out the acylation at 35℃ for 8h with a n:n: n ratio of 1:1:1.5 and with dimethylformamide as the solvating agent and the thinner.

文中对绿原酸进行了改性，即在碱性催化剂催化的条件下使其与月桂酰氯进行非水相的酯化反应，得到了脂溶性的绿原酸月桂酸酯。经过单因素及正交试验，确定了最佳合成条件为：以N，N-二甲基甲酰胺作为溶解、稀释剂，绿原酸／月桂酰氯／三乙胺的摩尔比为1：1：1.5，温度控制在35℃，反应时间8h。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。