继续进行

Methods The cells were divided into 5 treatment groups（10,25,50,75 and 100 μmol·L-1 QUE）, blank control and menstruum control group. The rat C6 cells were cultivated to 1×106·mL-1 in the RPMI 1640 medium, then added into 96 holes board with various doses of QUE by 3 holes per group,and MTT assay was used to observe the proliferation of the cells treated for 24,48 and 72 h. The change of cell cycle was also observed by flow cytometry after the cells were treated with 50 and 100 μmol·L-1 QUE for 48 h. The changes of the protein P53 and Bcl-2 of C6 cells treated with 50 μmol·L-1 QUE for 48 h were detected by immunocytochemical methods.

按QUE浓度分成10、25、50、75及100 μmol·L-15个处理组和空白对照组及溶剂对照组，大鼠脑胶质瘤C6细胞在RPMI 1640培养基中生长达1×106·mL-1后，在96孔板中分别加入上述浓度的QUE继续培养，每组设3复孔，作用24、48及72 h，采用MTT比色法检测QUE对大鼠脑胶质瘤C6细胞的增殖抑制情况，流式细胞术对50及100 μmol·L-1的QUE作用48 h的大鼠脑胶质瘤C6细胞进行周期分析，免疫组化法检测50 μmol·L-1的QUE作用48 h 的p53和bcl-2基因产物。

Sarkozy briskly snapped at the man and then moved on, continued to smile and shake hands with others along his path, saying "merci."

萨科奇激动的对这名男子进行了反击并继续前行，微笑着同旁边的其他人握手，并说道："谢谢"。

According to our method, we can quantify the total amount of isoflavone more rapid and conventiently without preparing all 12 kinds of standards.In this study, acetyl- and malonyl- glycosides were converted to 7-O-glycosides by reacting with 1M methanolic sodium methylate at 60℃ for 20 min. Then, the solution was neutralized with 2.5N HCL and extracted for further 30 min at 60℃. Aglycones and 7-O-glycosides were then analysed by HPLC.

根据本研究，此方法系使用1.0 M sodium methylate在60℃下与样品中之acetylglycoside、malonylglycoside反应20分钟，将其转化为7-O-glycoside，反应完成后以2.5N盐酸水溶液中和，调整溶液为80%甲醇溶液并在60℃下继续萃取30分钟然后进行HPLC分析；由於仅需要分析两种形式的大豆异黄酮化合物（aglycones及7-O-glycosides），因此可以有效地缩短分析时间至30分钟以内。

They say they will continue to work on the missile defense issue.

他们说，他们将继续就导弹防御问题进行磋商。

The model is continued by imposing a polar coordinate system on the moving reference frame.

通过把极坐标系作为该运动参照系而继续对模式进行积分。

If Iran is serious about taking practical steps to address the international community's deep concerns about its nuclear program, we will continue to engage both multilaterally and bilaterally to discuss the full range of issues that have divided Iran and the United States for too long.

如果伊 朗确实打算基于国际社会对其核项目的深切关注采取切实措施，我们将继续与伊朗进行多边和双边接触，商讨长期以来造成伊朗和美国之间分歧的全部议题。

"The government welcomes foreign investment in Australia and I will continue to ensure that investments are consistent with Australia's national interest," Wayne Swan, treasurer, said yesterday."In approving Sinosteel's application, I have determined that a shareholding of up to 49.9 per cent in Murchison will maintain diversity of ownership within the midwest region."

"政府欢迎外国在澳大利亚进行投资，而我将继续确保这些投资符合澳大利亚的国家利益，"澳大利亚财长韦恩·斯旺昨日表示："在审批中钢集团申请的过程中我已判定，允许其控制Murchison不多于49.9%的股权，将保持中西部地区企业所有权的多元化。"

If he continues on the same path, comparisons with Shunsuke Nakamura and Hidetoshi Nakata will be common and success in a major European league looks likely.

如果他能继续当前的表现，那么将他同中村俊辅以及中田英寿进行比较的声音将不绝于耳，而他在欧洲主要联赛的成功也指日可待。

Xavier Niel, the founder of Iliad, a young broadband firm which has upended France's internet-access market and wants to do the same for mobile telephony, says he is prepared for any attempts at intimidation.

伊利亚特公司的创始人Xavier Niel说，任何人企图对他进行威胁，他都乐意奉陪。他创办的这家年轻的宽带公司已经把法国的网站介入市场搅了个底朝天，还打算继续进军移动通讯领域。

I was proceeding along the High Street in a northerly direction.

我正在沿着大街向北走去。2。进行；继续下去

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。