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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在桑葚胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

An optimistic view would be to believe that the aftermaths of the Mumbai attacks would lead to a transformation in the thinking of the people where one would be compelled to pursue the protests resulting in an affirmative action for the welfare of the society, thus I wait and watch the end of this rising or rather hope that this upstart continues...

一种乐观的看法是认为后遗症孟买的袭击将导致变革的思想，一个人在那里将被迫继续抗议导致扶持行动的福利社会，因此，我等待观看本月底或上升，而希望这种暴发户继续。。。

The cost of this is usually enough to deter them, allowing the official slate of directors to retain their lucrative boardroom sinecures uncontested—even if only a tiny proportion of shareholders actually vote for them.

但是这些费用足以使他们望而却步，以此，董事会里在职的人员就算只有很少的股东的投票也可以继续成为候选人，从而继续拥有清闲又高薪的职位。

But they kept the business going and kept their customers happy throughout the war years by supplying good, tasty bread and oatcakes.

但他们在战争的年代继续保持着生意的运行和继续向他们的客户提供产品，好味的饼干和燕麦硬饼。

Peterson had only 20 Marines still on their feet when George Company passed through his position to continue the attack against Hill 1520. George and How Company were both down to two-platoon strengths.

当G连路过彼得森的阵地继续向1520高地发起进攻时彼得森的手下只剩下了20名还能够继续战斗的陆战队员。G连和H连也都只剩下了两个排的兵力。

Alternatively, Goldman could keep its old riches and newfound official status, keep on taking more financial risk, and try to square the circle by convincing people that it is a utility that operates in the public interest.

或者，高盛可以继续保持其原有的赚钱部门和新得到的官方身份，继续承担更多金融风险，并努力完成不可能完成的任务，即让人们相信，它是以公共利益为目的进行运营的公用事业机构。

But in a multi-polar, multi-speed financial world, the less developed financial industries of Asia and the Middle East continue to show strong demand for skilled professionals, and good business growth for many training organisations.

但在一个多极化、多速度的金融世界，亚洲和中东较不发达的金融业继续表现出对高技能专业人士的强劲需求，许多培训机构也继续呈现出良好的业务增长态势。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。