绦虫

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI （http:///www.ncbi.nlm.nih.gov/）, ExPaSy （http://www.expasy.org/） and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心（NCBI，http：∥www.ncbi.nlm.nih.gov/）和瑞士生物信息学研究所的蛋白分析专家系统（ExPASY，http：∥ca.expasy.org/）中有关基因和蛋白的序列和结构信息分析的各种工具，结合其它生物信息学分析软件包，从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Results of studies displayed: 1. We first constructed successfully cDNA expression library of the Oncosphere development stage of Teania solium.

结果表明： 1。利用质粒表达载体pSPORT 1首次成功构建了猪带绦虫六钩蚴cDNA表达文库。

When the adult film section of the main discharging send when the film festival palace containing eggs have been completed Oncosphere development.

绦虫纲有些动物的幼虫。当成虫的节片排出寄主体时，节片子宫内的卵已含有发育完成的六钩蚴。

It consist the three developments of Teania solium, Oncosphere and Cysticercus cellulosae, that the Oncosphere takes an important role in inbreaking body and causing response of immunology.

猪带绦虫的生活史由成虫、六钩蚴和囊尾蚴三个发育阶段组成，六钩蚴是囊尾蚴的早期发育阶段，在入侵宿主及引起机体免疫学反应等方面扮演重要角色。

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,ChinaAbstract: To obtain the hybridoma cell line specific to 45W-4BX antigen of Taenia solium oncosphere,the recombinant plasmid pGEX-4BX containing TSO45W-4BX gene was highly expressed in Escherichia coli induced with IPTG.

为获得针对猪带绦虫六钩蚴45W-4BX抗原的杂交瘤细胞株，用IPTG对TSO45W-4BX的重组质粒pGEX-4BX进行了诱导表达，并采用Sepharose-4B层析技术对表达产物进行纯化，运用SDS-PAGE和Western-blot分别对其纯度和活性进行了检测。

Granulosus in Xinjiang and there was no nucleic acid sequence difference of Eg95 antigen among the protoscolex, oncosphere and adult worm of E. granulosus.

结论Eg95基因在新疆株细粒棘球绦虫不同发育阶段均有表达，其基因序列无差异。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物，用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因，将其克隆至pUCm-T载体、测序并进行序列分析。

Purposes:Anti-worm medicine, Schistosoma japonicum, Schistosoma mansoni and Schistosoma Egypt, Clonorchis sinensis, paragonimiasis, ginger insects, tapeworm and cysticercosis been effective.

用途：抗蠕虫药，对日本血吸虫、曼氏血吸虫和埃及血吸虫、华支睾吸虫、肺吸虫、姜片虫、绦虫和囊虫都有效。

Questionnairing, skin test, serological test and eosinophil examination were carried out for Paragonimus infection.

带绦虫/囊尾蚴病调查，采用问卷调查和血清学检测，对血清阳性者再用Kato-Katz法检查带绦虫卵。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。