给药

Methods the spf mice were treated with med or optimized med in dose of 29.3 g/kg and 18.6 g/kg per day respectively by intragastric administration for five days continuously. and then, the various dysmnesia mouse models were prepared respectively with scopolamine (3 mg/kg), cycloheximide (120 mg/kg) by intraperitoneal injection and 40% alcohol (0.1 ml/10 g body weight) by intrag astric administration on the sixth day.

将强记汤及天然脑活素分别以 29.3 g/kg和 18.6 g/kg 的量每日经口灌胃给予 spf 级小鼠，连续 5 d，第 6 天给药 1 h 后制备成记忆障碍模型，第 7 天给药 0.5~1 h 后分别用跳台法和避暗法测试记忆成绩；另用不同剂量（50 g/ml 和 150 g/ml）天然脑活素水煎液处理大鼠大脑 b104 cns 神经细胞，以荧光素酶方法检测 creb 表达水平。

Methods The SPF mice were treated with MED or optimized MED in dose of 29.3 g/kg and 18.6 g/kg per day respectively by intragastric administration for five days continuously. And then, the various dysmnesia mouse models were prepared respectively with Scopolamine (3 mg/kg), Cycloheximide (120 mg/kg) by intraperitoneal injection and 40% alcohol (0.1 mL/10 g body weight) by intrag astric administration on the sixth day. Next day, memory result was estimated by using Step-down Test and Dark-avoiding Test after the mice were administered for half or an hour.

将强记汤及天然脑活素分别以 29.3 g/kg和 18.6 g/kg 的量每日经口灌胃给予 SPF 级小鼠，连续 5 d，第 6 天给药 1 h 后制备成记忆障碍模型，第 7 天给药 0.5~1 h 后分别用跳台法和避暗法测试记忆成绩；另用不同剂量（50 g/mL 和 150 g/mL）天然脑活素水煎液处理大鼠大脑 B104 CNS 神经细胞，以荧光素酶方法检测 CREB 表达水平。

Expression of nNOS mRNA, eNOS mRNA and iNOS mRNA in the E 20 cerebral cortex: There were no obvious changes of expression of nNOS mRNA in ETU-injected and control groups. Expression of eNOS mRNA increased significantly in cerebral cortex of the encephalocoele group, And there were no obvious changes of expression of eNOS mRNA in cerebral cortex of the control group and the other ETU-injection groups.

六、不同组E20胎鼠大脑皮质中nNOS mRNA、eNOS mRNA和iNOS mRNA的表达 1、实时定量PCR检测发现对照组、给药无畸形组、脊柱裂组和脑膨出组E20胎鼠大脑皮质中nNOS mRNA表达无明显改变； 2、给药无畸形组、脊柱裂组和对照组胎鼠大脑皮质中eNOSmRNA表达无明显改变，而脑膨出组胎鼠大脑皮质中eNOS mRNA表达明显增多； 3、在各组未检测到iNOSmRNA表达。

A gene sequence of the anthropogenic adiponectin spherical fragment obtained by the invention absolutely accords with a known gene sequence, and compared with the full-length adiponectin, the hydrolyzed anthropogenic adiponectin spherical fragment has stronger biological activity, fast reaction after dosage, good bioavailability and better clinic application prospect, especially when the diabetes patient receives a recanalization treatment after a myocardial infarction, the anemically myocardial damage of the diabetes patients can be eased by the exogenetic dosage.

本发明公开了一种人源性脂联素球状片段用于治疗糖尿病缺血性心脏病药物的应用，本发明所得到的脂联素球状片段的基因序列与已知的基因序列完全相符，与全长的脂联素相比，其水解以后的脂联素球状片段有着更强的生物学活性，而且给药后反应迅速，生物利用度好，有着更好的临床应用前景，特别是对糖尿病患者在发生心肌梗死后接受血管再通治疗时，通过外源性给药，以减轻糖尿病患者缺血心肌损伤。

As well using flameless atomic absorption spectophotometer toanalyse the amount of platinum binding to DNA,we can fand that after 1hour at 37℃ the amount of platinum binding to DNA for BGC-823 acted byCDDP 20ug/ml in hypotonic solution was 30.1±6.2ng/mg·DNA,whilethe isotonic groups were 6.4±1.5ng/mg·DNA.

采用非火焰原子吸收光谱法检测DNA结合铂含量发现，BGC-823经20μg／ml CDDP，37℃作用1小时，低渗给药组DNA结合铂含量为30.1±6.2ng／mg·DNA，等渗给药组DNA结合铂含量为6.4±1.5ng／mg·DNA，两者差异显著(P＜0.05)，该结果表明低渗溶液能显著增强CDDP与其作用靶点—DNA的交联。

Part at giving drug before (T1), time of zymogen of the blood pressure of the 5 Min(T2) after giving drug, 30 Min(T3), 60 Min(T4) that monitor, serous cruor (enzymatic time of work of cruor of PT), fractionary activation , thrombin , fibrinogen and plaque computation .

分别于给药前(T1)，给药后5 min(T2)、30 min(T3)、60 min(T4)监测血压、血浆凝血酶原时间、部分激活凝血活酶时间、凝血酶时间、纤维蛋白原含量及血小板计数。

Methods］ninety femal sd rats,weight 230～280 gram,were rangdonmized into three groups: qiangguyin group,miacalcic group and control group.all rats were variectomized.after 10 weeks,the osteoporosis model succeed,the treatment group,contrast group were given qiangguyin,miacalcic respectively.the treatment group used qiangguyin everyday,1ml/g.the contrast group used miacalcic 0.72u/kg,everyday hyodermic.and 3,4.5,6 months after the treatment,kill 10 rats to explore.the neck of femur were cut and stined with masson golder trichrome for bone histomorphomemetric contrast group analysis.

方法］健康雌性sd大鼠90只，体重230～280g。随机分为3组，分别为治疗组、对照组和空白组，每组30只。分别采取切除双侧卵巢方法进行骨质疏松造模，10周造模成功后，开始给药，治疗组用自拟强骨饮灌胃，每日一次，每次1ml/g，对照组用密盖息，皮下注射，每日1次，每次0.72u/kg。空白组，不做处理。在给药后3、4.5、6个月每组各取10只大鼠进行检测，先测量体重，空气栓塞处死，分离股骨颈，获得股骨颈样本，经切片等处理后，显微镜下作骨形态计量学检测。

Compared to the control animals, the rats treated with AC showed increased levels of citrate, 2-oxoglutarate and cis -aconitate, and decreased levels of hippurate and phenylacetylglycine in the urine. Such metabolic changes may have reflected, respectively, the changes in body energy metabolism and in the metabolism of intestinal flora in response to AC treatment.

结果表明灌胃给药后代表能量代谢的柠檬酸，2-酮戊二酸（2-oxoglutarate）和顺乌火酸（ cis -aconitate），反应肠道菌群代谢状况的马尿酸盐和苯乙尿酸出现异常，并且这些变化在给药后0~8 h达到最大，16 h后基本恢复正常。

The distributions of Hup A into rat brain tissues following intranasal dosing were compared with those after intravenous and intragastric dosing by tissue homogeneization.

结果大鼠鼻腔给药血浆AUC0→6h为静注的0.94倍，但脑脊液AUC0→6h为静注和灌胃的1.3和2.3倍；大鼠鼻腔给药后大脑、海马、小脑、左右嗅球的AUC0→6h分别为静注的1.5，1.3，1.0，1.2和1.0倍，为灌胃的2.7，2.2，1.9，3.1和2.6倍。

A kit for vaginal self-use by a female patient in protection against contracting viral infection and against conceptions as a result of normal heterosexual intercourse, said kit comprising a vaginal applicator comprising a vaginally insertable tubular member having a length of about five inches, and having an aperturable distal end adapted to be positioned adjacent to the patient's cervix when the applicator is fully inserted into the patient6's vagina, and at least one unit dose of the spermicidally and virucidally effective formulation of claim 1, said unit dose being form about 5-10 mls thereof.

一套女性患者阴道自用的工具，以防止感染病毒或因异性间正常性行为而受孕。该套工具包含一个阴道给药器（由一个长约5英寸、末端有孔眼、可插入阴道的管状构件构成，当给药器完全插入患者阴道后，构件的末端适合置于患者的宫颈附近），以及至少1个单位剂量（5-10毫升）的、能有效杀死精子和病菌的权利要求1中的配方。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了