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Mineralized matrix was determined by Alizarin Red S staining at 16 and 22 days after culture.

茜素红染色后显微镜下观察培养16,22 d 矿化结节形成

Gross observation and mass changes of the grafts: Two weeks after inoculation, there were obvious nod formations at the inoculated sites, but no nod formed in the blank control group.

移植物的大体观察与质量变化:接种2周后,同种异体软骨细胞-FasL组及同种异体软骨细胞组兔接种细胞-材料复合物处,均有明显的结节形成,对照组未形成接节。

Objective To investigate whether osteoblasts cultured in vitro can form mineralization nodes.

目的 了解体外培养成骨细胞的接种数量对钙化结节形成的影响。

The induced hmscs-tert were examined by oil red-o staining, alkaline phosphates staining and von kossa staining. results the hmsc-tert was negative for cd34, cd45, cd44, cd106 and cd166. after the induction, accumulated cytoplasmatic red lipid droplets were clearly visible in the adipose cells by oil red o staining, and alkaline phosphates-positive pitchy grains formed in the intracytoplasm and calcium nodes were found by von kossa staining in the osteoblasts.

结果 hmsc-tert细胞表面抗原检测cd34、cd45、cd44、cd106、cd166均为阴性;脂肪细胞定向诱导后油红o染色见胞浆内有红色脂肪颗粒,成骨细胞定向诱导后见胞浆内有碱性磷酸酶染色阳性黑褐色颗粒,von kossa染色见有钙结节形成

The greater the degree of exposure to silica and increasing length of exposure determine the amount of silicotic nodule formation and the degree of restrictive lung disease.

暴露在硅石中的程度和暴露时间的长短决定了矽结节形成的数量和限制性肺疾病的程度。

The pathological changes showed that the contusion, hemorrhagic focus and gliosis occurred in the injured brain areas.

病理检查见伤侧脑皮质有局部挫伤,蛛网膜下腔及脑实质有出血灶和胶质结节形成等改变。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group cnpared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early cnpared with the DMEM medium.

结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越多的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

Cultured osteoblasts at suitable cell density can easily form mineralization nodes in vitro, and β-glycerophosphate sodium and ascorbic acid just promote formation of mineralization nodes.

体外培养成骨细胞钙化结节的形成须接种适宜的细胞数量,用条件培养液培养细胞能促进钙化结节形成

Results There were more mineralization nodes to form in shorter time in the 1×105/ml cell density group compared with in the 2×105/ml cell density group, but in the 5×105/ml cell density group, there were not, and with the DMEM medium contained β-glycerophosphate sodium and ascorbic acid, mineralization nodes formed more early compared with the DMEM medium.

结果 在培养30天内,条件培养液或常规培养液培养的5×105个/ml组细胞无结节形成;而2×105个/ml组和1×105个/ml组都有钙化结节形成,而且有细胞越少形成结节越早,数量越多的趋势;给予条件培养液又较常规培养液培养有结节形成早而多的趋势。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

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这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。