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The operation on the skin and the conjunctiva was performed without using microscope, the dermis and the aponeurosis of the levator muscle of upper eyelid were limited within the ring of the ligature by the line of suture ligatured to form firmly heal which keeps the formed double-fold eyeli...

其法的特征是皮肤面和结膜面均在直视下操作,增加了可信度,缝线结扎后保证了将皮肤的真皮层和提上睑肌的腱膜层均拦在结扎线环内,形成了牢固的粘连愈合,使之形成的重睑沟牢固持久,永不消失。

The increased intraocular pressure is the most basic factor for damage to visual function,observation of bulbar conjunctival microcirculation may provide additionally more valuable information for diagnosis of POAG.

在POAG的发病中,眼压升高仍是最基本的视功能损害因素,观察球结膜微循环改变也可为POAG的诊断提供更多的有价值的病理信息。

Orthogonal experiment under special condition showed that modifier M has marked effect on properties of no bake sand. Other factors such as modifier B,phosphate concentration and aluminum hydroxide have some impact on properties of binder. It can be found by experiments that strengths of no bake sand are raised notably by binder that modified by modifier B. Modifier B can reduce the strength of no bake sand at high room temperature apart from condition of low room temperature that it can accelerate the reaction and strengthen the sand. Modest content of modifier M in binder has some improvement to anti-wettability of sand. But modifier B has not such notable effect. Electronic microscope shows that near cracks in no bake product there are fibrous shape substances formed when the samples are moistured in high humidity air for sometime.

通过改性剂B对粘结剂自硬砂的性能影响试验发现,改性剂B能显著提高粘结剂自硬砂的强度,在0℃左右很低的环境温度下改性剂M有利于提高自硬砂的反应速度和砂型强度外,但在较高环境温度下,改性剂M均降低粘结剂自硬砂的强度,但是适量的改性剂M有利于改善自硬砂的抗吸湿性,而改性剂B对于改善自硬砂的抗吸湿性的作用不如改性剂M明显,电镜观察发现抗吸湿性较好的自硬砂在高湿度下存放一定时间后,在粘结膜裂纹处会形成一种绒毛状物质,该物质对裂纹具有愈合作用,这种绒毛状物质中具有改性剂M中的某种成分,且含量很高,由此说明改性剂M对提高抗吸湿性有利。

Printing the work piece is placed on a fully open and the air during the oxygen ions of chemical reaction process, motivate a offsctdruckereien resin curing inks for drying, change business card printing and membership card making ink layer inside the conjunctuval and epiderm don't do it.

印品工件全面开放式的置于期间,空气中的氧气离子化的化学反应过程,能促使胶印树脂油墨的干燥固化,改变制卡和会员卡制作后油墨印层表皮结膜干燥而内部不干的状况。

In four patients with bilateral total corneal stem-cell deficiencies, 3-by-3-mm specimens of oral mucosal tissue were harvested, and the harvested cells were cultured for two weeks with 3T3 feeder cells that had been treated with mitomycin C. Conjunctival fibrovascular tissue was surgically excised from the ocular surface, and sheets of cultured autologous cells were transplanted directly to denuded corneal surface of one eye of each patient, without using sutures.

在4位双侧干细胞完全缺失的病患,取下3乘3的口腔黏膜组织样本,而且被取下的细胞与使用3T3细胞铺成的细胞层以mitomycin C处理,共同培养2个星期;结膜纤维血管组织利用外科从角膜表面切除,而且培养的自体细胞直接地移植到每位病患一只眼睛裸露的表面,不需要缝合。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

Methods The 3-5 generation fibroblasts were inoculated in 24-well with rat-tail collagen. A denuded area was made when the 3 generation of the fibroblasts was influent into a monolayer. Cells were then exposed to the presence of various concentrations of viper venom, mitomycin C, and the cells migrated into the denuded area were counted evely 6 hours.

将培养的第3~5代人结膜下成纤维细胞接种于涂有鼠尾胶原的24孔板中,待细胞生长融合后在培养板上划线,造成无细胞的裸露区,分别加人不同浓度的蛇毒制剂、丝裂霉素C及二者的混合液,每6h观察记录移行至裸露区的细胞。

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