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These reaction include conjunctival hyperemia and oedema,iritis,corneal neovascularization and oedema of the peripheral cornea and contraction generated during the corneal stroma remodeling. To make a dynamic state observation,one white rabbit of every experimental group was sacrified respectively at 15 days and 45 days postoperative.

在术后105天的观察时间内,观察并记录各组家兔角膜对SIS的反应,包括结膜的充血水肿、角膜血管化、虹膜炎、植片周边角膜水肿以及角膜基质重塑过程中所产生的收缩程度。

In the other group, muscle slippage occurred in 2 cases, bulbar conjunctiva hyperemia, subconjunctival hemorrhage and edema occurred in 40 cases, conjunctival scar occurred in 10 cases, conjunctiva cyst 2 cases and conjunctival polypus 3 cases.

直视下斜视矫正术组:术后发生肌肉滑脱的2例,球结膜伤口充血、结膜下出血、水肿者40例,结膜瘢痕10例,结膜囊肿2例,结膜息肉3例。

make the crude oil circumgyrate in a rotundity container in which the crude oil can get certain pressure, then let the crude oil out and put diesel oil in it to circumgyrate, note the degree of the diesel oil at different stage .

试验方法:使原油在高压釜内旋转模拟原油在管道中流动,在不同条件下形成沉积膜,再用等量的柴油进行冲刷,分阶段测量并记录柴油的色度,通过该色度指标的变化规律来分析影响结膜的因素。

It is an ideal conjunctival succedaneum for conjunctiva in conjunctiva transplantation and could be used as a graft to reconstruct ocular surface as well.

结膜ECM为目前比较理想的结膜替代材料,并可有效地用于角膜、结膜表面重建。

METHODS: Rabbit's taphococcus aureus conjunctivitis models and cantharides tincture conjunctivitis models were made to observe different groups of animalslsymptom of eye stimulation, negative rate of bacteria and conjunctiva histopathology.

建立兔眼损伤金葡菌性结膜炎及斑蝥酊刺激性结膜炎模型,观察各组动物的眼刺激症状、细菌转阴率及结膜的组织病理学改变,并和氯霉素滴眼剂或舒目滴眼剂的治疗效果相比较。

The objective of this study is to investigate the localization of the conjunctival stem cells in adults.

进一步研究结膜干细胞的确切定位及生物学特性、体外分离培养结膜干细胞、用组织工程构建生物结膜是结膜干细胞的研究方向。

Methods Twelve patients (12 eyes) with sever symblepharon and pseudo-pterygium were treated for symblepharon resection, then autologous oral mucosa was transplanted for reconstruction of the palpebral conjunctiva and fonix, amniotic membrane and conjunctival limbal grafts for bulbar conjunctiva and corneal surface reconstruction.

方法对12例12眼有严重睑球粘连及假性胬内的患者,进行睑球粘连松解后,应用自体口唇粘膜移植进行睑结膜和穹窿结膜修复,羊膜和自体结角膜缘组织瓣移植重建球结膜和角膜表面,观察其临床效果。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明,处理后4周,全周角膜缘上皮板层手术切除,中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化,未发生睑球粘连,角膜基质胶原纤维完整未见溃疡、穿孔等病变,细胞印迹学检查为结膜表型,可作为实验性角膜缘干细胞移植的病理模型;全周角膜缘上皮板层手术切除,中央角膜上皮用生理盐水擦除的5只试验家兔,有2只为结膜表型,另3只为角膜表型,观察期内结果不稳定;半周角膜缘上皮板层手术切除,中央角膜上皮层用生理盐水擦除的5只试验家兔,角膜表面透明,全部为角膜表型;直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔,有4只角膜基质胶原纤维断裂、溶解,并伴有严重的溃疡、穿孔、睑球粘连等病变,不能用于移植试验,另1只角膜表面透明,未见结膜和新生血管长入,细胞印迹学检查为角膜表型。

Immunohistochemical identification of cultured conjunctival cellsWhen the second cultured generation of conjunctival cells close to confluence, the glass coverslips were removed in situ fixation by methanol and DAB color immunohistochemical staining by CK13 monoclonal antibody in time.3. growth curve of conjunctival cellsThe 1st,3rd,5th passage cells were selectecd randomly and subcultured at the density of 5×10~4/ml,after culturing for another 1-9 days, were counted and compared the cell number every day.

采用消化后组织块法培养结膜原代细胞,并进行传代。2、培养结膜细胞的免疫组化鉴定把第2代的结膜细胞接种于置在培养皿中的盖玻片上,当细胞接近融合时,及时取出作原位甲醇固定,CK13单抗、DAB显色免疫组化染色。3、结膜细胞生长曲线的测定随机选取第1、3、5传代细胞,以5×10~4/ml密度传代、分别培养1-9天,每天分别细胞计数并进行分析比较,重复3次。

Conjunctivitis, which is usually called pink eye, is inflammation of the conjunctiva.

&红眼病&是传染性结膜炎的俗称,指的是结膜的发炎。

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