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结缔组织

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Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞:弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等;腺上皮下的基底膜区有少量结缔组织存在;扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况;透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞;近基底膜区观察到富含粗面内质网的细胞;基底膜间为薄层疏松结缔组织,内含肌细胞及神经末梢等结构。

Connective tissue disease is a group and the human immune response of multiple organ of the multi-system inflammatory connective tissue disease, the main lesion is myxedema, fiber-like degeneration and necrosis vasculitis.

结缔组织病(connective tissue disease, CTD)是一组与免疫反应有关的人体多器官多系统结缔组织的炎症性疾病,其主要病变为粘液性水肿、纤维蛋白样变性及坏死性血管炎。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示,石蜡切片的鼻咽癌癌巢内,可见53BP2的强阳性表达信号,信号呈紫色颗粒状分布于胞核,核仁清晰可见;而在相同条件下,在慢性鼻咽炎组织和癌旁上皮的结缔组织,也可见弱表达信号,如淋巴细胞和平滑肌细胞;在增生的鳞状上皮细胞的核内,可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上,均无表达信号。

Weeks after operation, around 2 kinds of materials had obvious connective tissue. The histology slice observation, we discovered has connective tissue proliferation, and few inflammatory cell infiltration. And some osteoblast around the material arrangement regulation, around the material had a kind of ossein and the chondroid structure appearance.

术后4W,2种植入材料周围有明显的结缔组织包裹,组织学切片观察,发现有结缔组织增生,其间有炎性细胞浸润,有成骨细胞在材料周围规则排列,有异位成骨趋势,材料周围已有类骨质和软骨样结构出现。

The evolution fromdense connective tissue of perichondrial outmost layer to mature cartilaginous tissue showed a continuous evolving histokinetic pro-cess. The tracheal perichondrium was the originating site of adaptive cartilage regeneration.

从软骨膜最外层致密结缔组织到成熟软骨组织呈现一个连续的组织演化动力学过程;气管软骨组织适应性再生的细胞来源是软骨膜结缔组织细胞。

It indicated that in the developmental biologic point of view,very archaic anatomical structure-loose connective tissue play an athletic and energetic role in organisms\' metabolic process.As cell signal transduction is concerned, subcutaneous fascia of acupoint is unspecialized.

体液等调节以外,筋膜结缔组织支架的存在可能构成另外一个较为原始的调控网络。3、在正常的疏松结缔组织中,ERK细胞信号转导蛋白一直有非磷酸化和磷酸化的表达,而这种表达又与细胞的增值和分化有关。

And the overgrowth lesions are characterized by epithelial acanthosis, with long, branched rete peg formation, and the excessive accumulation of radiating collagen fibers in the connective tissue layer.

此种疾病在组织学上的特徵为:上皮细胞层数有增厚的发现,并呈现明显的棘状凸出延伸至结缔组织中,在结缔组织中则有大量胶原蛋白堆积而产生纤维化;然而引发牙龈增生的病理机制至今仍不明确。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周,实验组可见珊瑚羟基磷灰石表面有细胞生长,孔隙内有结缔组织长入;对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成,孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成;对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成,部分区域可见髓腔样结构形成,并有血管长入;对照组仍未见新骨及骨样组织形成。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

Objective: Detect mRNA encoding TSHR in retroorbital fatty and connective tissue with TAO and healthy subjects, demonstrate that TSHR may present in retroorbital tissue and serve as common antigen in the thyroidal and extrathyroidal manifestations of TAO.

目的:寻找在甲状腺外组织TAO患者球后脂肪结缔组织中编码促甲状腺激素受体的mRNA,证实TSHR作为甲状腺和TAO球后脂肪结缔组织的共同抗原而存在于甲状腺外结构中。

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