结核病学

Use of the recombinant 38-kDa antigen of Mycobacterium tuberculosis as an immunogen for specific antisera production.

陆学东等［11］研究发现正常人和临床非结核病患者血清中存在结核分支杆菌某些抗原成份的抗体，主要在67 000以上大分子区域，与本文结果一致。

The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, including immunology, immunogenetics, pathogenetics, microbiology, microbial physiology, pathogenesis, pathology, molecular epidemiology, diagnostics, vaccine development and drug resistance etc.

该期刊出版有关宿主反应和结核病免疫学以及分子生物学、机体的遗传学和生理学等方面的原文和评论，包括免疫学、免疫遗传学、病理遗传学、微生物学、微生物生理学、发病机理、病理学、分子流行病学、诊断学、疫苗发展以及药物抗性等。

Results The recombinant MPT 64 protein existed in inclusion bodies of E.coli.and amounted to 20%- 30% of totle cell protein.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

These results indicated that the recombinant PEPCK might be one of the suitable antigens for serodiagnosis of tuberculosis.

结核杆菌PEPCK具有较好的免疫原性和抗原性，有可能作为结核病血清学诊断的一组抗原之一。

Objective To obtain the recombinant MPT64 protein,to study its immunological characteristics,and to evaluate its potential value fo r serodiagnosis of tuberculosis.

目的获得重组MPT64蛋白，研究其免疫学特性，评价其在结核病血清学诊断中的价值。

To construct the recombinant plasmid of protein 38kDa of M. tuberculosis and express, purificate and renaturate fusion protein 38kDa and to evaluate its potential value for TB serodiagnosis.

目的 构建结核分枝杆菌38kDa的重组质粒，在大肠杆菌中表达、纯化和复性重组蛋白，并评价其在结核病血清学诊断方面的价值。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体，在大肠杆菌 BL21（DE3）中进行诱导表达，并对表达产物进行纯化和复性，以获得大量ESAT-6P1抗原，同时对重组多免疫反应性进行鉴定评价，为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

Work at the bacteriologic, immunologic and molecular biological researchs of tuberculosis.

主要致力于结核病细菌学、免疫学和分子生物学研究。

Debris necrosis of lymph node is not the specific changes of KD. In the tuberculosis of lymph node,① debris necrosis is obvious, but it tends to be caseous nercrosis;② histiocytes, macrophages and foam cells are hyperplasia in the necrotic area or lymph sinus, moreover, epithelioid cells can be found and they tend to be form granuloma;③ numbers of neutrophils infiltrate in the necrotic area;④ the etiologic assay of acid fast bacterium tuberculosis bacterium is positive;⑤ typical clinical manifestation is insufficient.

淋巴结碎屑性坏死并非KD特有病变，诊断KD需先除外有明显碎屑性坏死的淋巴结结核病，后者主要表现：①碎屑性坏死虽明显，但趋于干酪样坏死；②坏死区内或同时在淋巴窦内，组织细胞、巨噬细胞和泡沫细胞增生，并演变为上皮样细胞和趋于肉芽肿形成；③坏死灶内、外可有数量不等的中性粒细胞浸润；④抗酸杆菌／结核杆菌病原学检测阳性；⑤缺乏KD的典型临床过程。

The traditional bacteriological methods need a long time or only have a poor positive detection rate for pulmonary TB especially extrapulmonary TB diagnosis.

目前，传统的细菌学检测方法需时较长，阳性率较低，在结核病特别是肺外结核诊断上仍然存在许多问题。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。