结核杆菌

The most well-known pasteurization process is associated with fluid milk, where the minimum process is that necessary to eliminate human health concerns associated with Brucellosis andor Tuberculosis.

巴氏杀菌过程最经常应用在液态乳上，是除去乳中与人体健康相关的布鲁氏菌和或结核杆菌而采用的最低强度的处理。

Antituberculous therapy was started empirically and cultures of the abscess confirmed the presence of Mycobacterium tuberculosis 3 weeks later.

针对这位病例预防性投与抗结核菌药物，而3星期后脓疡培养证实为结核杆菌感染。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

The average infection rate of the subjects is 74.17%. Men are more prone to tubercle bacillus infection than women. Income is one of factors associated with the infection.

本组受试对象的结核杆菌平均感染率74.17%；男性比女性更容易受到结核杆菌感染；经济是与感染有关的一个因素；"项目县"的实施，特别是线索调查对降低结核杆菌感染率是有明显效果的。

Objective In order to realize tubercle bacillus infection status in adults of Huai'an, to find out its reason, and to probe into the way of decreasing the infection rate of tubercle bacillus.

目的 了解我市成人结核杆菌感染情况，分析原因，探讨如何更好地降低结核杆菌感染率。

Men are more prone to tubercle bacillus infection than women. Income is one of factors associated with the infection. The implementation of tuberculosis control program,especially the clue investigation of tuberculosis patients have had an obvious effect in decreasing the infection rate of tubercle bacillus.

本组受试对象的结核杆菌平均感染率74.17%；男性比女性更容易受到结核杆菌感染；经济是与感染有关的一个因素；"项目县"的实施，特别是线索调查对降低结核杆菌感染率是有明显效果的。

Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

He was finally cured with a 6-month course of antituberculous chemotherapy. We conclude that tuberculosis should be considered in infants or young children with pneumonia that presents radiologically as pneumatocele formation, especially in whom there has been no response to empiric antibiotic therapy.

由此病例报告显示，结核杆菌可引起婴儿或较小儿童的肺炎，并可能并发肺中形成，当此类病人对广产性抗治疗没有反应时，务必将结核杆菌感染列入鉴别诊断。

Members of the HSP60 and HSP70 families represent major targets for antibodies in many infections with helminths, protozoa and bacteria. 10% to 20% of all T cells which respond to M. tb in infected mice are specifical for HSP65, this suggest that HSP65 is the major target for T cell when infection and give us a new thinking.

在多种感染中(包括蠕虫、原虫及微生物感染)，抗体应答是针对HSP60和HSP70家族的，结核杆菌感染小鼠10％～20％的T细胞细胞应答是针对HSP65的，提示HSP65是结核杆菌感染时T细胞攻击的主要对象，这为疫苗的开发提供了一种新思路。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题，我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质，在保证消化效果的同时不会由于NaOH过强的作用杀死细菌（时间控制在20分钟内），亦不会影响磁性颗粒的作用；通过应用自制定量模拟临床标本，我们比较了抗酸染色和金胺O荧光染色的检出限，金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目，金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间，高于抗酸染色的0.05个麦氏单位，前者敏感度较高；另外，经过磁性纳米颗粒富集作用后的标本涂片，在镜下显示其结核杆菌数量明显高于未经过富集的标本。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。