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结核分枝杆菌

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aim: to establish a prediction strategy for mycobacterium tuberculosis (h37rv) secreted proteins to pave the way for further research. methods: the whole protome of h37rv was scanned by signalp and tmhmm. the protein date analysis system based on visual foxpro was established to process the output of signalp and tmhmm and identify the secreted proteins. the sequences of the secreted proteins were aligned by blastp.

以signalp和tmhmm两个软件对结核分枝杆菌蛋白组进行扫描,基于visual foxpro构建&蛋白质数据分析处理系统&对扫描原始数据进行分析处理以识别分泌性蛋白,再经blastp完成相似性比对。

Technology of PCR of conclusion fluorescence ration is the n/med tuberculosis with a kind of fast, sensitive, strong particularity ramose bacili assists diagnostic method.

结论荧光定量PCR技术是一种快速、敏感、特异性强的结核分枝杆菌辅助诊断方法。

The method passes technical appraisement of fluorescent and mensurable PCR 350 individual plant are clinical and separate individual plant, undertake the bacterium of ramose bacili plants n/med tuberculosis appraisal and quantitative analysis, undertake fighting acerbity coloring and culture method at the same time.

方法通过荧光定量PCR技术鉴定350株临床分离株,进行结核分枝杆菌的菌种鉴定和定量分析,同时进行抗酸染色和培养法。

A sensitive, specifical and rapid method of touchdown PCR was established for detection of Mycohacterium from rhesus monkey.

建立了从猴全血中直接检测猴结核分枝杆菌DNA的Touchdown PCR方法,具有敏感性和特异性高,且简便的优点。

Objective: to determine the evidence of culture and sensitivity in lung tuberculoma for guiding clinical treatment of lung tuberculoma.

目的:通过研究分析肺结核球中结核分枝杆菌培养和药物敏感实验结果,指导肺结核球的临床治疗。

Arising from the combination of Mycobacterium tuberculosis is a zoonotic infectious diseases.

结核分枝杆菌引起的结核病是一种人畜共患的传染病。

Tuberculosis is a chronic respiratory infectious disease which imperils the health of human beings.

结核病是一种严重危害人类健康的慢性呼吸道传染病,其病原是结核分枝杆菌

Tuberculosis with Real time-FQ-PCR, acid-fast staining and culture had been done as contrast control at the same time.

目的:探讨FQ-PCR技术在检测临床标本中结核分枝杆菌的应用价值。

Results the positive rates and specificity of fluorescence quantitative pcr, acid-fast stain,were100% and 30% respectively.

结果 荧光定量 pcr、抗酸染色法检测结核分枝杆菌的特异性分别为100%、30%。

Methods: Several results obtained by MB/BacT system were analyzed, including the recovery rate of acid-fast bacilli, the contamination rate and the mean time for positive reporting from clinical specimens.

通过对仪器检出报阳时间、阳性率、污染率及药敏结果的分析,了解仪器的效能及结核分枝杆菌的耐药情况。

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