结核分枝杆菌

Objective To evaluate the value of protein chip for clinical detection of multiple Mycobacterium tuberculosis antigen.

目的 验证抗结核分枝杆菌多种抗原的抗体检测蛋白芯片的临床应用价值。

Objective To know the primary drug-resistance of Mycobacterium tuberculosis in Guangdong province, and to provide reference for tuberculosis control.

目的了解广东省结核分枝杆菌临床分离株的原发耐药性情况，为今后结核病防治工作提供参考依据。

Amplification of DNA of Mycobacterium tuberculosis from peripheral blood of patients with pulmonary tuberculosis.

肺结核患者外周血白细胞中结核分枝杆菌DNA的检测。

Objective To study the application of gene chip in detecting Mycobacterium tuberculosis resistant to rifampin and isoniazid.

目的 研究基因芯片在检测结核分枝杆菌利福平和异烟肼耐药性中的应用。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分，(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况；(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法，分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况；(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价；(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用；(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

The Company's subsidiary, Designer Diagnostics, is engaged in the research and development of diagnostic test kits designed to be used for the rapid identification of infectious diseases such as Paratuberculosis and Mycobacterium avium-intracellulare.

该公司的附属公司，设计师诊断，从事研究和诊断医疗的发展要为传染病的快速识别使用，例如副结核病和结核分枝杆菌胞内。

Bovine paratuberculosis is caused by Mycobacterium paratuberculosis and it is a kind ofchronic wasting disease in ruminants globally, which is called Johnes disease.

副结核病是由副结核分枝杆菌引起的一种全球性以反刍动物为主的慢性、消耗性传染病，也称Johnes。

The expression protein was analyzed by using the Western-blot, which proved that it had theantigenic activity of Mycobacterium paratuberculosis.

经免疫印迹分析证实，该融合蛋白具有副结核分枝杆菌抗原性。

Studies show the M. paratuberculosis secretes immunological competent protein in short termculture and opposites in long term culture.

研究表明，在短期培养过程中，副结核分枝杆菌分泌具有免疫活性的蛋白质，而在长期培养则相反。

The research results and expression product could serve as a basis for further studies on the gene-engineering vaccine, subunit vaccine and nucleonicacid vaccine against Bovine Paratuberculosis.

为进一步研究副结核分枝杆菌基因工程亚单位疫苗、核酸疫苗及诊断试剂奠定了基础。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。