结核分枝杆菌

Objective: To explore the characteristics of mycobacterium dependend antituberculous drug.

目的：探讨结核分枝杆菌依赖抗结核药物的发生率及特点。

Objective:To determinate drug resistance of tubercle bacillus by employing urease test.

目的：利用尿素酶试验的方法对结核分枝杆菌的耐药性进行检测。

Mycobacterium tuberculosis is the causative agent of tuberculosis.

结核分枝杆菌是造成结核病的致病菌。

It is concluded that the intracutaneous vaccination with the genome DNA of mycobacterium tuberculosis H37Ra strain induces activation of macrophages and generates strong immune responses, and this effect is not significant difference from immunizing with BCG.

结核分枝杆菌国际标准无毒株H37Ra菌株基因组DNA小鼠皮内接种后，能诱导小鼠腹腔巨噬细胞活化并产生较强的抗结核免疫效应，且该效应与卡介苗菌无明显差异。

Methods Mycobacterium tuberculosis strains, totally 158 isolates, isolated from Beijing, HeiLongJiang, Guangdong tinner Mongolia and Tibet, were subjected to IS6110-RFLP ,Spoligotyping and MIRU.

从北京市、黑龙江省、广东省、内蒙古自治区、西藏自治区五个地区收集158株结核分枝杆菌临床分离株，应用IS6110-RFLP、Spoligotyping及MIRU三种分型方法进行鉴定。

Tuberculosis is an infectious disease worldwide with high morbidity and mortality, largely owing to persistence of tubercle bacillus and inadequacy of the current chemotherapy.

结核病仍为最具破坏性的细菌性疾病之一，发病率和死亡率都很高。结核分枝杆菌能侵入宿主免疫系统，在肺肉芽肿中持留，致使目前的抗结核药物无法杀灭菌体。

EMB-resistance in 18 isolates was caused by the mutation at coden 306 in embB gene with positive rate of 62.1%. The PCR result was negative in 20 specimen from pulmonary diseases without active TB and 5 negative control strains of no MTB. The data in this study showed high specificity of 100%. Only one specimen in 7 specimen with negative result in acid-fast stain and cultivation was shown alteration in coden 306 in embB gene.

结果：130例活动性肺结核患者痰标本抗酸染色阳性38例，阳性率为29.2%(38/130)；罗氏培养阳性为79例，阳性率为60.8%(79/130)。79例培养阳性痰标本中耐乙胺丁醇29例，其中有18例在embB基因met306ATG突变，基因突变率为62.1%。20例非结核肺部疾病患者痰标本和5株非结核分枝杆菌菌株经套式PCR扩增全部为阴性，特异性为100%。7例涂阴培阴PCR扩增阳性的序列中有1例在embB基因306位密码了发生突变。

Tuberculosis from laboratories of 12 county tuberculosis dispensaries in Shandong were cultured and typed by MIRU genotyping in the KICID laboratory.

目的对结核分枝杆菌散在分布重复单位（mycobacterial interspersed repetitive units，MIRU）基因分型技术在山东省局部地区的应用进行评价。

The suptum samples from 102 tuberculosis patients and 45 non-tuberculosis patients were detected by fluorescence quantitative pcr and acid-fast stain.

对102例培养涂阳的结核痰液标本和45例非结核感染者的痰标本，应用荧光定量pcr法、痰涂片抗酸染色法检测结核分枝杆菌。

Methods FNAC different features of 68 cases with lymphatic tuberculosis were observed,acid-fast stain and PCR results were recorded,then positive rate was calculated.

方法观察68例体表淋巴结结核的针吸细胞学特征，记录抗酸染色情况并利用PCR技术检测穿刺组织中的结核分枝杆菌的DNA，计算阳性率。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。