结核分枝杆菌

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Objective: To investigate the in-vitro interferon-gammarelease assay based on different Mycobacterium tuberculosis antigens in the diagnosis of tuberculosis and of latent tubercular infections, and to compare it with the tuberculin skin test.

目的 探讨三种结核分枝杆菌抗原刺激外周血单个核细胞后γ干扰素检测对于结核分枝杆菌潜伏感染及结核病的诊断意义，并与结核菌素皮试进行比较。

We observed marophages pre-treated with ConA spread more rapidly on the well bottom compared with PBS injected controls under phase-contrast microscope. SEM of macrophages treated with ConA showed marked larger size and many surface ridges or ruffles and fine fingerlike processes or filopodia in comparison to controls, surface of which were smooth.

结果表明：①ConA处理后，倒置显微镜下可见Mφ粘附性增强；扫描电子显微镜下见其体积增大，指状突起增多；透射电子显微镜下见Mφ内线粒体等细胞器增多，伸出伪足包绕结核分枝杆菌并内吞，ConA活化的Mφ吞噬结核分枝杆菌数量增多，菌体的完整性受损。

Objective A prospective study was organized to determine the accuracy and costeffectiveness of the TB AccuProbe DNA hybridization test,the PCR bases LiPA assay,and a verifying automated DNA sequencing of the rpoβ gene,for the routine rapid and direct identification of the Mycobacterium tuberculosis complex and Non Tuberculosis Mycobacterium and rifampin susceptibility.

目的用 3种先进的技术-TBAccuProbeDNA杂交法、INNO LIPATMRIF TB探针试验和rpoβDNA基因序列测定的前瞻性研究，以鉴定结核分枝杆菌复合物和非结核分枝杆菌及其成本效益。

Objective To investigate the feasibility of multiplex PCR for rapid identification of Mycobacterium tuberculosis and non-tuberculosis Mycobacteria.

目的 探讨应用多重PCR技术快速鉴定结核分枝杆菌与非结核分枝杆菌的可靠性。

Surgical debridement and anti-Mycobacterium therapy is the treatment of the nontuberculous mycobacteria hand infection in an effective way to.

手术清创和抗分枝杆菌联合治疗是治疗手部非结核分枝杆菌感染的有效途径。

Methods From June 2005 to March 2008, treated 20 cases of the nontuberculous mycobacteria hand infection patients after the start of functional rehabilitation treatment time were divided into Group A, B, each of 10 cases, both surgical debridement and anti-Mycobacterium therapy, rewirable after the first day until the wound for wound healing time, follow-up to 6 months after the operation, observe the wound healing time and the recurrence; according to the joint activity score standard score comparison group A, B group of before and after six months of the functional differences.

对2005年6月～2008年3月收治的20例手部非结核分枝杆菌感染患者按术后开始进行功能康复治疗的时间随机分为A组（术后1周）、B组（术后3周），每组10例，均行手术清除病灶和抗分枝杆菌治疗，以术后第一日起至伤口拆线为伤口愈合时间，随访到术后6个月，观察伤口愈合时间、复发情况；根据关节活动度评分标准评分比较A组、B组术前与术后6个月功能的差异。

We concluded that sputum cultures in the Taipei Hospital Branches for Communicable Disease Control revealed that 4.06% were multi-drug resistant strains of Mycobacterium tuberculosis and a very high percentage of multi-drug resistance in Nontuberculous Mycobacterium, which requires the attention of health workers.

研究分析结果显示台北市立联合医院疾病管制院区所培养分离的菌株中结核分枝杆菌多重性抗药菌比例达4.06%，但非典型结核分枝杆菌抗药性之菌株比例却非常的高，值得密切的注意。

Methods Targeting the most common mutation types at codon 526 and codon 531 of Rifampiein Resistance Determining Region of rpoB gene from Mycobacterium tuberculosis ,labeled probes （526CAC, 526TAC, 531TCG and 531TTG） were designed to detect 38 Rifampin-resistant clinical isolates with known RRDR sequence, 24 Rifampin-sensitive clinical isolates with wild type sequence of RRDR and 5 nontuberculous mycobacteria isolates, then a method using real-time PCR was established.

方法针对结核分枝杆菌rpoB基因利福平耐药决定区（Rifampicin Resistance Determining Region，RRDR）526密码子和531密码子常见的突变形式设计探针（526CAC，526TAC，531TCG和531TTG），应用已知rpoB基因RRDR区序列的38株利福平耐药临床分离株和24株利福平敏感临床分离株以及5株非结核分枝杆菌菌株建立荧光定量PCR检测基因突变的方法。

Objective To discuss the assay methods and clinical value of IgG antibody to Mycobacterium Tuberculosis. Methods To detect the antibody levels of tuberculoprotein 16KDa, 38KDa and LAM by Protein Chip and ELISA together, the sensitivity, specificity and total coincidence rate of the two methods were compared.

目的 探讨结核分枝杆菌IgG抗体的检测及其临床应用价值方法应用蛋白芯片识别仪分析结核病人血清中结核菌蛋白16Kda和38Kda以及脂阿拉伯甘露糖抗体含量，同时与ELISA法进行比较，判断其敏感性、特异性和总符合率。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。