结核

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

Crinoids, ammonoids and bivalves dwelt on the stems and lived on pseudoplanktons. They were gradually deposited on the sea bottom with increasing biomass of the dwelling organisms and lime content, and at last concretions were formed.

海百合、双壳类和菊石类栖息在植物茎干上营假浮游生活，随着栖息生物量和化学沉积物灰泥的增加，逐渐沉积在海底形成结核。

Cysticerci meningitis is a rare type of serous meningitis. It should be differentiated with other meningitis,such as tuberculous meningitis, cryptococcal meningitis and syphilitic meningitis.

囊虫性脑膜炎是一种少见浆液性脑膜炎，应与结核性脑膜炎、霉菌性脑膜炎、梅毒性脑膜炎等相鉴别。

Cysticerci meningitis is a rare type of serous meningitis. It should be differentiated with other meningitis,such as tuberculous meningitis, cryptococcal meningitis and syphilitic meningitis.

囊虫性脑膜炎是少见浆液性脑膜炎，应与结核性脑膜炎、霉菌性脑膜炎、梅毒性脑膜炎等相鉴别。

Objective: To study the value of interferon gamma in differentiating tuberculosis from malignant pleural effusions.

目的；研究胸腔积液γ-干扰素水平时结核性与癌性胸腔积液鉴别诊断的价值。

RANTES play a different role of T cell抯 immune response in tuberculous and malignant pleural effusions.

趋化因子在结核和肿瘤中发挥不同的T细胞免疫应答，为以后的应用奠定实验基础。

Results L-form of M.tuberculosis was induced by the concentrations of routine inhibition test of rifampin, isoniazid or ethambutol.

结果 结核分支杆菌在常规药敏试验浓度的利福平、异烟肼、乙胺丁醇的作用下均可形成L型。

Ethambutol is the key intermediate of ethambutol hydrochloride which is used as a chiral antituberculosis agent.

对手性抗结核药物盐酸乙胺丁醇的关键中间体———乙胺丁醇的合成进行了系统研究。

Under the impression of disseminated LV, an antituberculous therapy comprising isoniazid and ethambutol was prescribed.

在符合寻常性狼疮的诊断之下，给予抗结核药物isoniazid及ethambutol，其病灶在两个月内逐渐愈合。

Tuberculosis H37Rv were treated with no drugs, rifampin isoniazid, ethambutol. streptomycin and ofloxacin.

2应用定量RT-PCR方法检测53株结核分枝杆菌临床分离株对利福平的敏感性。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。