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METHODS: The whole protome of H37Rv was scanned by SignalP and TMHMM. The protein date analysis system based on Visual FoxPro was established to process the output of SignalP and TMHMM and identify the secreted proteins. The sequences of the secreted proteins were aligned by BLASTp.

以SignalP和TMHMM两个软件对结核分枝杆菌蛋白组进行扫描,基于Visual FoxPro构建&蛋白质数据分析处理系统&对扫描原始数据进行分析处理以识别分泌性蛋白,再经BLASTp完成相似性比对。

aim: to establish a prediction strategy for mycobacterium tuberculosis (h37rv) secreted proteins to pave the way for further research. methods: the whole protome of h37rv was scanned by signalp and tmhmm. the protein date analysis system based on visual foxpro was established to process the output of signalp and tmhmm and identify the secreted proteins. the sequences of the secreted proteins were aligned by blastp.

以signalp和tmhmm两个软件对结核分枝杆菌蛋白组进行扫描,基于visual foxpro构建&蛋白质数据分析处理系统&对扫描原始数据进行分析处理以识别分泌性蛋白,再经blastp完成相似性比对。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then. Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库,采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法,研究了西太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系,得到了一些与&暖池&区环境特点紧密相关的新发现,新认识。

Psychrophilic and psychro-tolerant bacteria were cultured and used for screening the cold-active enzymes. Some characteristics of cold-active protease and chitinase were analyzed then.Microbial 16S rDNA clone libraries of deep sea sediments were constructed and studied by PCR-RFLP (Restriction Fragment Length Polymorphism) and phylogenetic analysis based on 16S rDNA sequences.

通过构建沉积物中微生物16S rDNA克隆文库,采用PCR-RFLP分析、DNA-DNA杂交、16S rDNA序列测定以及系统发育分析的方法,研究了两太平洋&暖池&区和东太平洋&结核&区两个不同海区深海沉积物中的微生物多样性和群落结构特征及其与环境的相互关系,得到了一些与&暖池&区环境特点紧密相关的新发现,新认识。

Technology of PCR of conclusion fluorescence ration is the n/med tuberculosis with a kind of fast, sensitive, strong particularity ramose bacili assists diagnostic method.

结论荧光定量PCR技术是一种快速、敏感、特异性强的结核分枝杆菌辅助诊断方法。

The method passes technical appraisement of fluorescent and mensurable PCR 350 individual plant are clinical and separate individual plant, undertake the bacterium of ramose bacili plants n/med tuberculosis appraisal and quantitative analysis, undertake fighting acerbity coloring and culture method at the same time.

方法通过荧光定量PCR技术鉴定350株临床分离株,进行结核分枝杆菌的菌种鉴定和定量分析,同时进行抗酸染色和培养法。

Experiments indicated that the oxidative ability of ocean manganese nodules is stronger than pure MnO 2 and the reductibility of aniline is stronger than phenol.

实验表明,大洋锰结核的氧化能力强于纯的MnO2,苯胺的还原性强于苯酚。

Tuberculous lesions were identified in 27 cattle (47.4%; n = 57) with the evidence on gross and/or microscopic examination in 1 or more sites. The most frequently affected site was retropharyngeal lymph node, followed by mediastinal lymph node, hilar lymph node, and mesenteric lymph node.

结果显示,所收集的结核菌素检测阳性牛中,有 27 头(47.4%; n = 57)在肉眼或/及显微检查下发现有结核样病灶,其中最常出现病变的位置为咽背淋巴结,其次依序为纵膈淋巴结、肺门淋巴结和肠系膜淋巴结。

Chest roentgenographic were normal, shape, atelectasis, keratosis pheumonitis, swelling of hitum of lung etc.

支气管镜活检和刷检是目前诊断气管、支气管结核最可靠和最准确的方法,值得临床医师推广应用。

Vitamin D was used to treat TB in the pre-antibiotic era, when special sanatoria were built in sunny locations, such as the Swiss Alps.

维生素D在抗生素之前的时期用于治疗结核,那时像瑞士阿尔卑斯山那样的专门的疗养院建立在阳光充足的位置。

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