结核

The allergy quarantine with avian bacillustuberculosis PPD indicated that there is significantly difference between the pVAXl-hsp65,pVAX1-hsp65-10 groups and supersonic antigen of MAP group (P＜0.01).This indicates that the Hsp65 andHsp 10 does not interfere with detection of Paratuberculosis allergy.

禽结核杆菌PPD皮试结果显示，pVAX1-hsp65和pVAX1-hsp65-10免疫组与副结核分枝杆菌超声抗原免疫组差异极显著(P＜0.01)，表明Hsp65和Hsp10不干扰变态反应检测。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

In order to develop an indirect ELISA for diagnosing bovine paratuberculosis, the DNA fragmentsof map086 2and map2154c were amplified from the genome DNA Mycobacterium avium Subspeciesparatuberculosis K10. Then the DNA fragments of map0862 and map2154c were spliced byoverlapping extension, yielding a fusion gene map0

为了建立一种有效的牛副结核病间接ELISA方法，本研究设计了两对引物，从副结核分枝杆菌P_(18)株的基因组中PCR扩增出两个目的基因map0862和map2154c、，采用重叠延伸剪接技术(splice by overlapping extension，SOE)获得融合基因map0

The lab is capable of quarantine hazardous biology such as : Phytophthora sojea , Tilletia controversa , Bursaphelenchus xylophilus , Sorghum halepense , Acanthoscelides obtectus , Bean Pod mottle virus , and practising diagnosis akabane disease , leukaemia bovum,paratuberculosis and other hazardous animal disease;detection of listeria monocytogenes , staphylococcus aureus and salmonella etc in food;examination of transgenic products such as soybean , rapeseed , corn etc and detection of bovine ,sheep and goat -derived for preventing bovine spongiferm encephalopathy .

检测的项目主要有：大豆疫病、小麦矮腥黑穗病菌、松材线虫、假高梁、菜豆象、菜豆英斑驳病毒等植物危险性有害生物，赤羽病、白血病、副结核等动物危险性有害生物，单增李斯特氏菌，金黄色葡萄球菌，沙门氏菌等致病性食品微生物，大豆、油菜籽、玉米等转基因产品，以及预防疯牛病的牛羊源成分检测等。

Objective To evaluate the value of percutaneous peritoneal biopsy in diagnosis of tuberculosis peritonitis.

目的评价经皮腹腔穿刺腹膜活检对结核性腹膜炎的诊断价值。

Objective: To investigate CT features of tuberculous peritonitis.

目的： 探讨结核性腹膜炎的CT表现。

Objective: To analyze the clinical characteristics of tuberculosis peritonitis and explore the diagnostic methods.

目的 分析结核性腹膜炎的临床特点，探讨其诊断方法。

Objective To investigate the diagnostic strategies and treatment of cirrhosis complicated with tuberculous peritonitis.

目的 探讨肝硬化合并结核性腹膜炎的诊断和治疗策略。

Methods:Analyze 196 cases who had tuberculosis peritonitis diagnoded in our hospital retrospectively.

回顾分析196例住院确诊的结核性腹膜炎病例。

The drug tolerance rate had no obvious difference between the permanent citizens and temporary population of Bao'an district of Shenzhen.

户籍人口与暂住人口对抗结核药的耐药性无明显差异。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新