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Over 90 per cent of patients at Newham University Hospital and Northwick Park Hospital in London with TB were found to have a vitamin D deficiency.

超过百分之九十的患者在纽汉大学医院和northwick公园医院在伦敦同结核病人发现有维生素d缺乏。

Polymarase Chain Reaction may be the better method of detecting M.tuberculosis than other methods and lead us to carry out a series of research on this problem. This thesis is composed of five parts:(1)systematic evaluation for the first time of the ability of five defferent PCR methods to detect M. tuberculosis;(2) using the endonuclease and southern blot by a radioactive and a nonradioactive labeled probe, to confirm the specificity of the PCR products and to analyse the sensitivity of detection by southern blot method using digoxigenin-labeled probe;(3) evaluation of acid-fast staining, bacterial culture and PCR for the detection of M. tuberculosis in sputum, pleural and peritoneal effusion samples;(4) The feasibility of PCR for detection of M tuberculosis on formalin-fixed paraffin embedded tissues;(5) 26 cases of sarcoidosis were retrospectively studiedwith PCR for M. tuberculosis and nontuberculosis Mycobacterium and the problems on histopatholoical diagnosis of sarcoidosis are discussed.

论文共分为五个部分,(1)首次系统地比较了五种不同引物序列的PCR检测结核杆菌方法的特异性和敏感性等情况;(2)利用限制性内切酶酶切分析和同位素及非同位素标记DNA探针进行Southern杂交的办法,分析PCR产物的序列与原设计是否相符及杂交检测PCR产物对提高检测结核杆菌敏感性的情况;(3)并对PCR用于快速检测临床标本中的结核杆菌进行了初步评价;(4)探讨了PCR用于检测石蜡包埋组织中结核杆菌的作用;(5)并利用PCR检测结核杆菌和非结核分枝杆菌的方法对26例原病理诊断为结节病的病例进行了PCR检测及组织病理学诊断的探讨。

The rate of pleural effusion to diagnose is 88.9% under thoracoscopy:tuberculous pleurisy displays mainly pleura hairy nubble and diffuse white millet nubs and pleural thickeningthe trabe-form conglutination can been found; and metastatic tumor of pleura shows gray tuberculum impar and inequality of size nodosities with diffused pleural congestive and edema in the CPA and disphragmatic muscle.

结核性胸膜炎主要表现为胸膜多发结节,部分病灶出现弥漫性白色粟粒样结节和胸膜增厚,可见条索状粘连;而转移性胸膜肿瘤主要表现在肋膈角、横膈处的灰白色单结节和大小不等的多结节;非特性慢性炎症性胸膜炎主要为胸膜充血水肿,胸膜增厚纤维增生或粘连,少见有单个或多个结节增生。

The rate of pleural effusion to diagnose is 88.9% under thoracoscopy:tuberculous pleurisy displays mainly pleura hairy nubble and diffuse white millet nubs and pleural thickening,the trabe-form conglutination can been found; and metastatic tumor of pleura shows gray tuberculum impar and inequality of size nodosities with diffused pleural congestive and edema in the CPA and disphragmatic muscle.

结核性胸膜炎主要表现为胸膜多发结节,部分病灶出现弥漫性白色粟粒样结节和胸膜增厚,可见条索状粘连;而转移性胸膜肿瘤主要表现在肋膈角、横膈处的灰白色单结节和大小不等的多结节;非特性慢性炎症性胸膜炎主要为胸膜充血水肿,胸膜增厚纤维增生或粘连,少见有单个或多个结节增生。

The project period of two years from Nuwa AIDS Tuberculosis and Malaria specific implementation of the Asia-Pacific Alliance.

该项目为期两年,由女娲抗艾滋病结核和疟疾亚太联盟具体实施。

The structure of oolite layers and the recrystallization and replacement of concretes can be used to make comparative explanatio of the formation mechanism and growth genesis of uro-lith stones.

沉积鲕石、核形石的分层结构和再结晶交代现象等,可以对比应用于尿石的形成和发育机理的解释;同时,尿路结石形成理论中的局部过饱和以及取向附生学说,也可引进于沉积鲕石、核形石和结核的形成理论中。

Patient of tuberculosis of 67 bacterium shade is fighting the method the wool below bronchus lens goes to brush smear and alveolar lavement before n/med tuberculosis fluid smear looks for the bacili that fight acid, next orthoptic seeing the apparent mucous membrane hyperaemia, person that add the performance such as stricture of the thick accent that reach a canal lives all right check pathology examination.

方法67例菌阴肺结核患者在抗结核前行支气管镜下毛刷涂片和肺泡灌洗液涂片找抗酸杆菌,直视下见有明显的黏膜充血、增厚及管腔狭窄等表现者行活检病理检查。

MRI can demonstrate clearly the tuberculosis centrum osteitis, the intervertebral disc destroys, zhui side abscess and nerve harm.

MRI能清楚显示脊柱结核椎体骨炎,椎间盘破坏,椎旁脓肿及神经损害。

Results (1) Osteitis and destruction of end-plate showed long T1 and long T2.(2) Anterior and lateral abscess had long T1 and long T2 presenting even and structure-less signal.(3) The intervertebral discs showed normal or only degeneration at the early stage, and destroyed at later stage.(4) With Gd-DTPA enhancement, fibrotic granulation around abscess and destroyed area was well demonstrated.(5) Abscess in the bone and/or paraspinal abscess, destruction of end-plate were the important evidence for early diagnosis.

结果 (1)椎体骨炎及终板破坏呈长T1长T2信号,(2)椎旁或椎前冷脓肿呈长T1长T2均匀无结构信号,且上下跨越范围较大,(3)早期椎间盘信号正常或只出现退变,晚期则被破坏,(4)Gd-DTPA增强扫描可清楚显示冷脓肿周围纤维肉芽组织及椎管内侵犯,(5)骨内小脓肿和/或椎旁脓肿、椎体终板破坏是MRI诊断早期脊柱结核的重要依据。

Coli can overproduce 38 000 antigen of Mycobacterium tuberculosis. Inclusion bodies are easy to purify and can protect the recombinant antigen from protease, on the other hand, it has not biological activity unless the denatured protein is accurately folded.

构建的大肠杆菌重组体能高效表达结核分支杆菌38 000蛋白质抗原,包涵体的表达形式有利于蛋白质纯化和蛋白质稳定,蛋白质必须经过正确折叠才具有生物学活性。

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