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Real-time fluorescence quantitative PCR technique has been widely applied, such as mRNA expression, detections of DNA copy number and determination of mononucleotide polymorphism, as well as in the clinical medicine including accurate quantitative examination of mycobacterium tuberculosis, Type B and Type C hepatitis, AIDS virus, gonococcus, and chlamydia trachomatis.

实时荧光定量PCR的应用范围非常广泛,包括mRNA表达的研究、DNA拷贝数的检测、单核苷酸多态性的测定等,已广泛应用于医学临床,它能对结核分枝杆菌、乙型、丙型肝炎、爱滋病病毒、淋球菌、沙眼衣原体等病原体进行准确的定量检测。

Results showed that the topsoil structure of the CK treatment was dense with little porosity developed, and the soil microstructure was sandy granular fabric - granular fabric. In these treatments using chemical fertilizers only, the soil particles did not form soil structure with little porosity, and the soil microstructures in the N, NP or NPK treatment were observed as prophyropectic fabric-fine sand agglomerplasmic fabric, much better than the CK treatment. In those treatments receiving farm manure alone or farm manure plus chemical fertilizers, the quantity of groundmass increased obviously. The soil had a loosen structure, higher porosity, enriched animal and plant residua, iron-manganese nodules and humus, and more micro-aggregates developed. The soil microstructure of the MNPK treatment was pectized-compacted takyric fabric-intertextic fabric, the best microstructure observed among all the treatments.

结果表明:长期不施肥料的CK处理,土壤耕层结构致密,很少有孔隙发育,土壤微结构为结构较差的碎屑聚积状-沙粒聚积状;长期单施化学肥料,土壤颗粒未形成结构体,少孔隙,土壤微结构主要为斑晶胶凝状-细沙粒聚积状结构,土壤结构比CK处理优;长期施用有机肥或有机无机肥料配施的处理,土壤粗颗粒数量显著增加,结构疏松,孔隙量大,动、植物残体丰富,有铁锰结核和腐殖质的形成和微团聚体的发育,土壤微结构类型以MNPK处理最好,为胶凝紧实状-多孔状结构。

It may produce skin wound that appear like those of other diseases such as hansen's disease, cutaneous tuberculosis , syphilis , skin cancer and fungal infections.

它可能会产生皮肤伤口出现像其他疾病,如麻风病,皮肤结核,梅毒,皮肤癌和真菌感染。

Results 8 cases of 32 patients were misdiagnosed as tuberculosis,7 cases were misdiagnosed as pneumonia,6 cases were misdiagnosed as tuberculosis pleurisy,3 cases were misdiagnosed as omarthritis,2 cases were misdiagnosed as arthritis,2 cases were misdiagnosed as transient ischemic attack,2 cases were misdiagnosed as hemicrania,2 cases were misdiagnosed as intervertebral disk hernia.

结果 32例患者中,误诊为肺结核8例,肺炎7例,结核性胸膜炎6例,肩周炎3例,关节炎2例,短暂性脑缺血发作2例,偏头痛2例,椎间盘突出2例。

objective to investigate the reasons of misdiagnosis on the young patients with non-typical lung cancer,to enhancing the cognition of the disease.methods the data of incidence,clinical manifestation,imageology,bronchoscope,surgical pathologic examination of 32 cases with non-typical lung cancer were analyzed.results 8 cases of 32 patients were misdiagnosed as tuberculosis,7 cases were misdiagnosed as pneumonia,6 cases were misdiagnosed as tuberculosis pleurisy,3 cases were misdiagnosed as omarthritis,2 cases were misdiagnosed as arthritis,2 cases were misdiagnosed as transient ischemic attack,2 cases were misdiagnosed as hemicrania,2 cases were misdiagnosed as intervertebral disk hernia.conclusion the clinical symptom of young patients with non-typical lung cancer was non-typical,and the misdiagnosis rate was high.

目的 探讨不典型青年肺癌误诊的原因,以期提高对本病的认识。方法对32例误诊的青年肺癌(5岁)患者的发病情况、临床表现、影像学检查、支气管镜及手术病理检查进行分析。结果 32例患者中,误诊为肺结核8例,肺炎7例,结核性胸膜炎6例,肩周炎3例,关节炎2例,短暂性脑缺血发作2例,偏头痛2例,椎间盘突出2例。结论青年肺癌临床症状不典型,误诊率较高。

The time of hight incidence of gout, tuberculosis and traumatic arthritis is from 20 to 40 years old; peak age of rheumatoid arthritis is 40 to 60 years of age; and synovial chondromatosis, osteoarthritis, infectious arthritis lumbar hip disease caused the incidence of knee pain increases with age increased.

3痛风、结核及创伤性关节炎的高发年龄为20~40岁,类风湿性关节炎的高峰年龄为40~60岁,滑膜软骨瘤、骨性关节炎、感染性关节炎及腰髋部疾病造成膝关节疼痛的发病率随年龄的增长而升高。

objective: to study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization.

目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。

Methods Absolute concentration method was used for the detection of drug sensitivity to 4 antituberculsis agents : isonieotinyl hydrazide, rifampicin , streptomycin and ethambutol.

采用绝对浓度法进行异烟肼、利福平、链霉素、乙胺丁醇4种抗结核药物的耐受性测定。

Objective To study whether the blood plasma albumin level can influence the hydrocele absorbency for refractory tuberculo-pleurisy.

目的探讨血浆白蛋白水平与难治性渗出性结核性胸膜炎患者胸液吸收的关系。

Nitrosamines, fiberglass, hydrocyanic acid, thorium oxide, beryllium and other pulmonary diseases (such as tuberculosis and chemical substances and lipid aspiration pneumonia), could lead to pleural mesothelioma.

亚硝胺、玻璃纤维、氢氰酸、氧化钍、铍及其它肺部疾病(如结核和化学物质及脂质吸入性肺炎),均可导致胸膜间皮瘤。

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