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Chinensis and Kuruma prawn M. japonicus increased at 12 and 48 hours post-inoculum, but decreased sharply after 36 and 72 hours post-inoculum respectively. LPS inducible NOS activity also decreased significantly with the time elapsed in WSSV infected shrimps. Results of WSSV detection with the Dig-DNA Probe dot blot hybridization test showed that WSSV of the two penaeid species couldn't be detected until iNOS started decreasing.

在此基础上,我们建立了对虾血细胞中诱导型一氧化氮合成酶的鉴定方法—硝基四氮唑蓝还原法和血细胞形态法以及酶活力的测定方法—L-瓜氨酸分析法和亚硝酸盐分析法并优化了鉴定和测量条件,结果显示:对虾血细胞在4℃下与L-精氨酸和LPS孵育8h以及当L-精氨酸浓度为2.5mmol l〓LPS浓度为100μg ml〓,Ca〓浓度为2.5mmol l〓时,iNOS活力的测定结果最佳。

Based on the results of the large-size shaking table test of a subway station structure built in liquable site,considering the influence of the soil-structure nonlinear dynamic interaction by 2D finite element methods,the seismic responses of the soil- subway station structure system under different test conditions are analyzed by using ABAQUS software.

根据可液化土层上土-地铁车站结构动力相互作用大型振动台模型试验结果,以软件ABAQUS为平台,将地基土-地铁车站结构体系视为平面应变问题,采用记忆型嵌套面黏塑性动力本构模型模拟土体的动力特性,采用混凝土动塑性损伤模型模拟车站结构混凝土的动力特性,建立了土-地铁车站结构非线性动力相互作用的有限元分析模型,对各种试验工况下地基土-地铁车站结构体系的地震反应进行了数值模拟,并与试验结果进行了对比,结果表明:数值模拟与振动台模型试验结果基本一致,体现出了相似的规律性,相互印证了计算分析的力学建模和振动台试验结果的正确性。

Methods: We used DNCB paper method (DNCB solution absorbed in a small piece paper instead of DNCB acetone solution directly dropped on to the skin) to treat 90 cases of alopecia areata, 22 cases of verruca vulgaris, 140 cases of molluscum contagiosum, 120 cases of verruca plana and 6 cases of immuno related suppurative diseases for clinical analysis.

对采用DNCB法治疗的斑秃、寻常疣、传染性软疣、扁平疣的临床疗效及伴发化脓性疾患病例的随访结果进行回顾性分析。结果:斑秃治愈率为33.3%,扁平疣为35%,寻常疣及跖疣分别达56%及66%,传染性软疣为41.8%。

objective:to analyze the difference between mammary duct ectasiaand plasma cell mammitis.methods:the data of 24 cases of mde and 28 cases of pcm,including clinical manifestations,distinguished diagnoses,surgical therapy,pathological results,were analyzed.results:the clinical manifestations of mde are nipple discharge and breast mass,which should be distinguished with breast mass and early stage of breast cancer.the treatment is local resection.major pathological changes are duct ectasia and obvious periductal inflammatory changes.the clinical manifestations of pcm are breast mass and inflammatory changes,which should to be distinguished with advanced breast cancer and inflammatory breast cancer.the surgical treatment is extensive resection.the major pathological changes are inflammatory reaction and multiple abscesses in breast tissue.conclusion:mde and pcm are significant different,and they are different progressing stage of disease,thus the two diagnoses are independent.

目的:分析乳腺导管扩张症和浆细胞性乳腺炎临床上的差异。提出各自独立诊断的论据。方法:结合24例乳腺导管扩张症和28例浆细胞性乳腺炎对两病的临床症状、鉴别诊断、手术治疗、病理结果进行比较分析。结果:乳腺导管扩张症临床表现为乳头溢液和乳腺肿物,主要与乳腺肿物和早期乳腺癌鉴别诊断,手术以局部切除多见,病理表现为导管扩张及导管周围明显炎性改变。浆细胞性乳腺炎临床表现为乳腺肿物和炎性改变,主要与晚期乳腺癌和炎性乳腺癌鉴别诊断,手术切除的范围较大,病理变化以乳腺组织的炎性反应和多发性脓肿为主。结论:乳腺导管扩张症和浆细胞性乳腺炎有明显的差异,应作为这两种疾病独立诊断。

MethodsIn the solvent of 1 mol/L NaOH, using the reaction of the water-soluble phenolic acidity in Radix Salviae Miltiorrhizae and the NaNO_2-Al(NO_3)_3, the total components of water-soluble phenolic acidity in Radix Salviae Miltiorrhizae injections and drip solution(calculated with protocatechualdehyde) were determined by colorimetric s...

方法利用丹参中水溶性酚酸类成分与亚硝酸钠和硝酸铝溶液在碱性条件下的显色反应,比色法测定该两种制剂中水溶性酚酸的总量。结果原儿茶醛在3.336~14.46mg/L范围内呈良好的线性关系,回归方程为:A=0.02777C+0.01279(r=0.9996),加样平均回收率为100.8%,RSD为0.96%。结论该法简便快速,结果准确,重现性好,可用于该制剂的质量控制。

Result In the Identification of ISSR molecular markers, 3 primers in 20 of being chose had better amplification effect. Optimization results representative of C. grandis Osbeck showed that primers 826 for the optimum temperature of Rutaceae plants amplification was 52.0℃, and the best cycle time was 35 cycles.

结果]选用的20个引物中有3种引物扩增效果较好,以柚为代表的优化结果表明,引物826对芸香科植物扩增的最适退火温度为52.0℃,最佳循环次数为35个循环。

Results The results showed that the optimum carban and nitrogen resource were sucrose and lopassium niruate. The growth rates of mycelia were fastest at the temperature of 30 ℃ and pH value 6. Darkness was benefitial to hyphal growth, but light was benefitial to sclerotial formation.

结果]结果表明,白绢病菌菌丝生长最适温度为30℃,最适pH值为6,最适合的碳源、氮源为蔗糖和硝酸钾;黑暗条件有利于菌丝的生长,而光照条件有利于菌核的产生。

By synthesizing theories of systems engineering, science of talent, talent assessment, performance appraisal and bibliometrics, the author analyzes the qualification structure and performance structure of theachers in military academies and sets up a basic structure and concrete appraisal indicator system of qualifications of technical specialty for teachers in colleges. The author puts forward new appraisal indicators such as "lecuring hours coefficient","republication coefficient","thesis of core journals coefficient" and "citation coefficient". Comparatively complete quantification approaches are invented by the author for working performance of teaching, researching and social service of teachers. Based on this, the author establishes an integrated method to generate the appraisal results. By the application of stadardized processing on the appraisal results and synthetization of weighted indexes, the author solves the addibility and comparability problems of differentiated appraisal results by category or dimensiok, and realizes the amalgamation and comprehensive appraisal of inflexible appraisalresults and flexible appraisal results, and probes into the testification methods of validity, consistency and sensitivity analysis for the indicators system.

综合运用系统工程理论、人才学理论、人才素质测评理论、工作绩效评估理论、文献计量学等原理与方法,分析研究了军校教师的素质结构和绩效结构;建立了高校教师专业技术资格评审条件基本结构和具体评价指标体系,提出了&完成主讲课程课时系数&、&著作重版系数&、&核心期刊论文系数&、&论文被检索引用系数&等若干新的评价指标;提出了比较完整的教师的教学、科研、社会服务等工作业绩的量化方法;以此为基础,建立了评价结果的综合方法,通过对评价结果的标准化处理,运用指标加权综合法,解决了不同类别、不同量纲指标的评价结果的可加性和可比较性问题,实现了硬评价结果和软评价结果的融合和综合评价;探讨了指标体系有效性、一致性、灵敏度分析的检验方法。

The effects of solution\'s pH value, mol ratio between phenyl aldehyde and L-Arginine, concentrations of sodium chloride, ammonia chloride andL-Lystine, initial concentration of L-Arginine on precipitation rate of a -tolylene arginine were investigated, the results indicated that phenyl aldehyde precipitation method\'s appropriate pH value was higher than 11, suitable mol ratio between phenyl aldehyde and L-Arginine was 1.25, initial concentration of L-Arginine was must higher than 15g/L, sodium chloride had little effect on precipitation rate, on the contrary, ammonia chloride and L-Lystine had great effect on it, both of them must be eliminated; At the same time, adsorption isotherm at 25 C of L-Lystine on anion exchange resin A was measured, the result showed that the maximal equilibrium adsorbance was 30mg/g, the influences of solution\'s pH value, temperature, concentration of chloride ion on adsorption of L-Lystine by anion exchange resin A were also studied, the results indicated that appropriate operational pH value was 0.5 0.2, the effect of temperature on adsorption ratio was little, process could be operated at room temperature, the concentration of chloride ion must be eliminated.

考察了溶液pH值、苯甲醛与L-精氨酸摩尔比、氯化钠与氯化铵浓度、L-赖氨酸浓度、L-精氨酸初始浓度对苯甲醛沉淀L-精氨酸的影响,结果表明,沉淀反应的适宜pH值大于11,苯甲醛与L-精氨酸的适宜摩尔比为1.25,(来源:Aa6BC论文网www.abclunwen.com)L-精氨酸适宜的初始浓度在15g/L以上,氯化钠的存在对沉淀率基本上没有影响,而氯化铵和L-赖氨酸的存在使沉淀率下降;同时测定了25℃时阴离子交换树脂A吸附L-赖氨酸的吸附等温线,表明其最大平衡吸附量为30mg/g,考察了溶液pH值、温度、氯离子浓度对阴离子交换树脂A吸附脱除L-赖氨酸的影响,结果显示从L-精氨酸和L-赖氨酸混合液中吸附分离出L-赖氨酸的适宜pH值为10.5±0.2,温度对L-赖氨酸吸附率影响不大,吸附过程可在室温下进行,氯离子的存在使L-赖氨酸的吸附率降低。

The molecular weights of the two subunits were shown 142 KD for the larger one and 47 KD for the smaller one, respectively, on SDS—polyacrylamid electrophoresis. The molecular weight of the native vitellin was determined as 205 KD on native gradient polyacrylamid gel electrophoresis.

用蒸馏水沉淀法从东方粘虫Mythimna seperata新鲜卵中提取卵黄蛋白,SDS-PAGE分析结果显示,粘虫的卵黄蛋白由大小两个亚基组成,大亚基分子量为142KD,小亚基分子量为47KD,线性聚丙烯酰胺梯度凝胶电泳结果显示,卵黄蛋白的天然分子量为204KD。

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