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The results were showed that embryogenic calli were induced from young leaves, which were cultured on MS medium supplemented with 2,4-D 2mg/L and KT 0.2mg/L. For the proliferation of embryogenic calli, the suitable culture medium was MS+BA 8mg/L +NAA 1mg/L. The development and maturation of somatic embryo could be much improved by using the medium of MS+ZT 2mg/L or BA 5mg/L +IAA 0.2mg/L. For the induction of secondary somatic embryo from integral somatic embryo, the suitable culture medium was MS+KT 0.1mg/L+NAA 0.01 mg/L or MS+ZT 0.1mg/L+NAA 0.01 mg/L, the proliferation frequency is 214%, 256% respectively. The cotyledonary generated from somatic embryos of Aesculus hippocastanum L.

本文就欧洲七叶树的组织培养和体细胞胚发生以及植株再生进行了系统研究,主要结果如下:以植物细胞具有全能性的理论为依据,以欧洲七叶树幼嫩叶片为外植体,进行体细胞胚胎发生研究,研究结果表明,诱导愈伤组织的适宜培养基是MS+2,4-D 2mg/L+KT0.2mg/L,MS+BA 8mg/L+NAA 1mg/L有利于胚性愈伤组织的诱导和增殖,添加ZT 2mg/L或BA 5mg/L和IAA 0.2mg/L的MS培养基有利于体细胞胚发育和成熟,体细胞胚可直接诱导次生胚发生,MS+KT 0.1mg/L+NAA 0.01 mg/L或MS+ZT 0.1mg/L+NAA 0.01 mg/L培养基诱导效果最好,增殖频率分别为214%、256%。

The results Showed that twenty isolates have the following biological aspects:① Capability of forming capsule,② Growth at 37℃ within 3days,③ Forming brown colonies on Niger seed agar, CACA agar,④ Urea hydrolysis test positive within 96 hours.⑤ No blue colonies were found on CGB agar.⑥ The genus could be identified by ID32 identification kid, but the species could not.⑦ The sequence of the LSUrDNA D1/D2 region analyzed confirmed to be C. neoformans var neoformans.⑧ Serotype A and AD were found in these isolates, Serotype A was predominant(83.33%),⑨ Some biodiversities of isolates existed.

结果 20株菌皆具有以下特征:①形成英膜,②37℃3天内生长试验皆为阳性,③咖啡酸玉米琼脂和油菊籽培养基皆可以产生褐色菌落,④尿素酶试验96h皆呈阳性,⑤CGB培养基未见蓝色菌落生长,⑥ID32鉴定板可以鉴定到属,种的鉴定结果各不相同,⑦大亚基核糖体DNA的D1/D2区域序列分析证实皆为新生隐球菌的新生变种,⑧血清型仅见A和AD二型,以A型为多数(83.33%),⑨20株临床分离株随机扩增DNA多态性分析有一定的生态多样性。

Result The results showed that phenolic compounds of Canarium album Raeusch fruit mainly distributed in pulp, which total phenolic content was about 1.5%, and phenolic acids with structure characteristic of hydroxybenzoic. And gallic acid, ellagic acid were the main phenolic compounds in Canarium album Raeusch, their relative content were 38.8% and 14.3%, respectively.

结果]结果表明:橄榄果实中的酚类成分主要集中在果肉中,其总酚含量约为1.5%,橄榄中的酚类成分以含苯甲酰结构的酚酸类为主,其中主要的酚类化合物为没食子酸和鞣花酸,相对含量分别为38.8%和14.3%。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果:1合成的CS-PAA磁性聚合物微球呈球形,大小均一,TEM测定其干态下粒径为80nm左右,磁化曲线证实具有超顺磁性,具有一定的PH敏感性,固载表柔比星后,水溶液性状稳定,无沉淀物,DLS测定直径约200nm左右,测定载药率为15%,较文献报道高,包封率为20%。2肉眼观察试验组大鼠膀胱表面呈褐色,可见大量的Fe粒子聚集,普鲁士兰染色法显示,试验组大鼠膀胱壁内有大量的Fe粒子,分布至膀胱壁全层,与对照组大鼠相比,试验组大鼠的肝、脾内的Fe粒子聚集量明显降低;HPLC测定结果与Fe染色相同;高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高,其他组大鼠血生化指标没有明显变化。3MTT法发现,高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组,FCM发现试验药物组可引起明显的肿瘤细胞凋亡,试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C,试验组细胞Bcl-2蛋白减少,Bax蛋白变化不明显,Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P<0.01),其中高剂量组的抑制率最高。

A comparative study on the field attraction effects of different amounts on Chlorophorus diadema showed that fourteen test attractants had a certain trapping ability to Chlorophorus diadema, among which, D was the strongest, with a mean trappability of 0.82%; H took the second place, with a mean trappability of 0.64%; while G was the weakest, with a mean trappability of 0.11%.

本实验研究了14种引诱剂对槐绿虎天牛的诱捕效果,结果表明,14种引诱剂对槐绿虎天牛都有一定的引诱效果。野外诱捕结果显示:引诱剂D的效果最好,日平均诱捕率为0.82%;H次之,为0.64%;G最弱,日平均诱捕率为0.11%。

Results The positive percentage in superficial gastritis, deep gastritis, gastritic ulcer, duodenal ulcer, chronic atrophic gastritis and gastric cancer, were 70.3, 72.2, 77.7, 69.2 and 25 respectively by URT, 59.2, 77.8, 71.4, 70.7, 75 and 78 respectively by PCR, and 48.1, 61.1, 70.3, 70.7, 66.7, 66.6 and 72.2 respectively by cagA gene probe detection. Conclusions Statistics suggest that cagA+ H.

结果 浅表性胃炎、深度胃炎、胃溃疡、十二指肠溃疡、慢性萎缩性胃炎及胃癌病人的检测结果是:尿酶检测阳性百分率为:70.3、72.2、77.7、69.2、25;PCR阳性百分率分别为:59.2、77.8、71.4、70.7、75、78;基因探针杂交检测阳性率分别为:48.1、61.1、70.3、70.7、66.7、66.6、72.2。

Methods and Results 194 cases of genual osteoarthritis were randomly divided into tuina group, plaster compress group and combined group, The general effective rate of the tuina group is 80.00%, the plaster compress group is 81.25%; and that of the combined group is 88.54%, but the Control group is 80.56%.

方法与结果采用随机分组方法,将194例骨性膝关节炎患者分为观察组、推拿组和药敷组,研究结果表明:推拿结合药敷治疗组治疗的总有效率为88.54%,单纯推拿治疗组的总有效率为80.00%,药敷组的总有效率为81.25%。

Two strains of bacteria, named as TJ-1 and TJ-2, were isolated from enrichment with crude oil as a sole carbon source. Strain TJL-1 and Strain TJL-2 were isolated with hexadecane as a sole carbon source. The 16SrDNA identified results showed that the four strains of TJ-1, TJ-2, TJL-1 and TJL-2 were Bacillus pumilu , Acinetobacter venetianus , Oceanobacillus Picturae , Bacillus pumilus , respectively.

结果表明,利用2216E平板从以原油和正十六烷为碳源的富集液中各得到两株菌,分别为TJ-1、TJ-2和TJL-1和TJL-2,分子生物学方法鉴定结果表明,这4株菌分别为短小芽孢杆菌、威尼斯不动杆菌、 Oceanobacillus Picturae 和短小芽孢杆菌。

According to the distribution of species and amount, the author makes a PCA analysis to the different habitats. The analysis of hierarchical cluster to different habitat is according to the distribution of species. According to the results of the two ways of analysis and the factual condition of environment, the environment of Daqinggou is divided into 3 types of habitat that are described as the hygric plant community in the bottom of ditch, xerophytic plant community in the top of ditch and single type plant community.

以直翅目昆虫种类和数量的分布为属性对不同生境作PCA分析并以直翅目昆虫种类分布为属性作等级聚类分析,两种分析方法所得结果十分相似,结合分析结果和实际情况将大青沟7种不同生境分为3类,分别命名为沟底湿生植物群落,包括水曲柳植物群落、湖边湿地植物群落和稻田植物群落;沟上干早植物群落,包括沙丘草地和大果榆植物群落;单一型植物群落,包括人工针叶林植物群落和蒙古栋植物群落。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P<0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P<0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示:(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性,而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P<0.05);三组动物的支气管和肺组织常规细菌培养结果均为阴性;感染组动物透射电镜检查见肺泡间隔增宽,其中有较多胶原纤维堆积,其余两组则未见明显异常。

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