结果为

The study on the phylogenetic relations among these species is very useful for the study on genome and marker-assisted breeding in Brassica species. The karyotypic analysis is one of the most important methods in analysing the phylogenetic relation of species with closer relations. The protocol for inducing high frequency metaphase was established by pretreatment with low temperature and 8hydroxyquinoline, and then the preparation of chromosome and karyotypes analysis of six economical important Brassica species were carried out. According to the karyotypic analysis, it was shown that in the genomes of amphidiploid species, there are genomical components from didiploid species. Our result showed that B. campetris, B. juncea and B. napus belonged to class 2B, while B.

以二倍体B.nigra（B，n=8）基因组为探针，用B.campestris（A，n=10）或B.oleracea（C，n=9）的基因组进行封阻，分别与B.juncea（AB，n=18）和B.carinata（BC，n=17）进行GISH杂交发现，B.juncea和B.carinata基因组中仅有来自于B.nigra的8对染色体表现杂交信号，而其它染色体杂交信号较弱；以B.campestris或B.oleracea基因组为杂交探针与B.napus（AC，n=19）进行GISH杂交发现，所有19对染色体均出现杂交信号，根据杂交结果难以正确判断B.napus基因组中的哪些染色体是来自于A基因组，哪些是来自于C基因组；以rRNA基因（18S-5.8S-26S）为探针的FISH杂交结果表明，B.campestris基因组中rRNA基因位点为6对；B.oleracea为3对；B.nigra为2对；B.carinata为4对；B.juncea为6对；B.napus为6对。

In addition, some modifications on several computational methods are also presented. Using LMTO method the electronic structure of several systems are studied, and some results are obtained. They are: The ideal Nb (100) surface has three surface states, the multi-layer relaxed surface has two surface states. The surface energy of the ideal surface is higher than that of the relaxed surface, that means that the multi-layer relaxed surface is more stable than the former one, which supports the LEED results. The mono-layer relaxed Ag (111) surface is the most stable one among several" stable surface models"presented by several researchers. The surface energy of Ag (111) surface is higher than that of surface Ag (001), which supports some experimental results such as different reaction rate at different surface orientations for the same material. The surafce states of Si (111) surface not only locate near the Fermi level, but also in the valence band, which agrees well with Cohen's conclusion. Si (111)-H is an effective model for analysing the surface states and H adsorbed on the back surface is a good method for improving the convincingness of the results obtained on thinner slab models. The surface stability depends on three different kinds of MoSi〓(001) surfaces, the surface with mono-layer Si is the most stable one, and the surface with Mo at the first layer is the most unstable one among them. These are consistant with the Kemoda's experimental results. The valence bands of clean or K adsorbed CdTe (111) surface agrees well with the synchrotron radiation studies. The surface of CdTe (111) consists of four kinds of surface models which show different surface electronic structures and different surface structure stabilities. The conclusion agrees well with Wu's experimental work. The different absorbed alkali metals on the CdTe (111) surface give different adsorption characteristics which have relations not only with the valence electrons, but also with the core ones of the alkali metals. The electonic structures of Si-C alloys are different from that of Si-Ge alloys, and the energy band gaps of Si-C alloys do not increase linearly with Carbon concentration, our conclusion supports Alexander's results, but conflicts with Soref's one.

现分述如下： LMTO方法及其应用方面：1）通过对Nb（100）表面电子态分析发现清洁理想表面有三个表面态，多层弛豫表面有两个表面态；表面能大小说明多层弛豫表面更稳定，支持了LEED结果。2）通过对采用不同方法获得的几种不同Ag（111）表面稳定结构的表面能计算分析，给出了单层弛豫表面为Ag（111）表面的最稳定结构；从Ag（111）单层弛豫表面和Ag（001）表面的表面能比较，发现了Ag（001）表面表面能要比Ag（111）小的，表明了同种物质不同表面取向将表现出不同物理、化学性质，这是与实验中得出的结论是吻合的，3）通过对Si（111）表面态分析，不仅发现了Si（111）表面不仅具有居于费米能级附近的悬挂键所对应的表面态，而且还有很多表面态位于价带能量范围内，与Cohen等结果一致，H饱和slab模型背表面相当于增加了slab层的厚度，是一有效的变相增加slab层厚的方法，弛豫表面较清洁理想表面价带谱们低能端的少许移动，预示着总能降低，说明弛豫表面较清洁理想表面稳定。4）MoSi〓具有三种表面，从费米能级上态密度值大小得到单层Si表面最稳定，Mo原子为表层原子的表面最不稳定，双层Si原子表面居中的结论，这与Kemoda等人实验结果是一致的。5）通过对CdTe（111）表面表面电子态、表面结构稳定性及表面H、碱金属吸附的电子结构系列研究，不仅得出了CdTe（111）清洁及碱金属K吸附价带谱与同步辐射光电子谱相吻合的结果，而且发现了CdTe（111）表面具有四类不同原子近邻特征，表现出四类不同的表面结构及电子结构特征：不同表面态分布、不同的表面结构稳定性（表层原子与次层原子成三键有一悬挂键的表面要比表层原子与次层原子成一键有三悬挂键稳定（与Wu等人实验结果一致））、不同的H吸附特性。

The quality of interior environment in nursing home residents will become one of the attention-getting subjects due to the gradual increase of aging populations This paper directs at the research of nursing home's interior environment factors in Tainan We use the standard of international practice to analyze the intrinsical data of environmental monitoring and establish the characteristics which from environmental physical reaction of nursing home The research items are air environments (PM10 CO CO2 formaldehyde TVOC) temperate environments and light environment (illumination average brightness daylight factor) etc There are the inductive conclusions of this research: 1 In basic medicine the clinical diagnosis means the theory and technique about physical examination with patients The definition of clinical diagnosis in architecture should be the diagnosis of building preserve and improve the feasible way to extend building's service life and assure the residents of the healthy quality of interior environment The object of clinical diagnosis in architecture is not only to ensure the use of structure and environment quality but also keep the structure and environment quality in sustainable; therefore creating an efficient system of preserving plan is needed 2 It is an accurate and technical circumstance which about improving remedying and reconstructing the problems of structure space This circumstance includes finding questions ensuring causation ascertaining ultimate strength evaluating environment quality estimating the necessary of restoration and implementing on a suitable way Furthermore when reconstructing and ameliorating the appearance of structure space it should be respected and the memories of it should be preserved 3 In clinical diagnosis of environment quality of space the question of physical environment and the deficiencies of structure must be separated The structure space of construction and the high quality of space should be inspected strictly then observed the crucial reason that can find out any invisible and hidden causes The diagnosis of construction is to observe the reason of destroyed structure The environmental diagnosis of indoor and outdoor is to find out the crucial reason that influences health 4 In order to decrease the iterant problems the construction and environment of space should be diagnosed However the destruction by natural strength and artificial using by human are important causes that can affect the service life of construction Searching the cause of disease just like curing the patient which includes inspection diagnosis cure and prevention We tested the thermal conductivity of siding material in this research and then found out the roof east vertical face and west vertical face have most radiant heat especially the construction of RC The characteristics of opaquely outer casing have relations with the thermal conductivity of wall to wiz the ability of heat insulation Therefore increasing the efficiency of heat insulation and decreasing the endoergic factor of insolation are very important to temperate environments

而随著国内老年人人口逐渐增加，逐渐迈向高龄化之同时，对於高龄者使用空间之室内环境品质相关议题之探讨，亦成为未来我们需加以关注之议题之一。主要为针对台南某安养中心室内环境因子进行检测调查工作，利用现场室内环境测定以取得有效的实测数据；再藉由汇整相关国际评估基准，并进行实测数据比对分析，建立老人安养中心对物理环境反应之特性。并以前期相关研究文献回顾与老人安养中心实测案例现场的实验，来了解不同空间物理环境特性之影响，并将结果回馈实际建筑及室内设计之用。调查工作项目包含：空气环境之PM10、CO、CO2、甲醛、TVOC；温热环境之室内温度、相对湿度、人体PMV、PPD；光环境之照度、均齐度、昼光率等因子调查。本研究结果可归纳以下几点结论： 1 所谓临床诊断医学乃相对於基础医学而言，是指实际与病人接触的医疗及护理行为中牵涉到的理论和技术；而建筑的临床诊断的定义应为建筑本体与室内外诊断、维护及改善所有可行的方式来延长建筑物的寿命，并确保人与室内环境空间品质的健康。建筑临床诊断目的为确保结构体与环境品质能继续使用，而能维持所需要的效能及承受能力。一个有效的维护计画体系是必须建立的 2 在建筑空间之问题改善、治疗与修复执行中，是一个精确与技术性的事项，其中包含找出问题、确定原因、评估结构应力强度、评估环境品质、评估修复与改善必要性、选择及执行一个适当的过程。其改善与修复也必须尊重建筑空间原有色彩与形貌，并保留原有记忆及文化。 3 在临床诊断中空间环境品质中，物理性环境问题与结构构件缺陷必须分别判断，诊断建筑空间之结构与空间舒适品质必须详细观察其症结性问题，才能发现任何隐藏及有潜伏性缺陷的原因。建筑本体诊断是观察出建筑本体之破坏原因，而是内外空间环境诊断目的在於的找出影响健康因素之症结性问题。 4 建筑本体与空间环境之预防评估目的为降低与抑制问题的再发生。然而，自然力的破坏与人为使用因素皆是影响建筑生命期之主要因素，找出病源问题就如同医生对病人的处理，包含了侦查、诊断、治疗以及预防。 5 本研究以外墙材料热传导率测试结果发现，屋面与东西向立面是承受辐射热最多的地方，尤以 RC 的建筑更甚，不透明部分的外壳能特性主要与壁体的热传透率即隔热能力有关，但是承受来自日射的吸热影响，因此增加隔热性能与降低日射吸热因子是对温热环境是非常重要的。

Result The correlation between the BME degree and the collapse of ONFH: the collapse rate was 4.5% in degreeⅠ, 11.8% in degreeⅡ 66. 7% indegree Ⅲ, 90% in degree Ⅳ. The correlation between the BME degree and the Harris:93.0±5.41 in degree Ⅰ, 84.1±5.42 in degree Ⅱ, 76.4±4.22 in degreeⅢ, 66.3+7.46 in degree Ⅳ.

结果］骨髓水肿程度与塌陷相关性结果：Ⅰ级水肿塌陷率为4.5%，Ⅱ级水肿塌陷率为11.8%，Ⅲ级水肿塌陷率为66.7%，Ⅳ级水肿塌陷率为90%：骨髓水肿程度与Harris评分相关性结果：Ⅰ级水肿评分为93.0±5.41分，Ⅱ级水肿评分为84.1±5.42分，III级水肿评分为76.4±4.22分，Ⅳ级水肿评分为66.3±7.46分。

The results showed that the average heterozygosity was higher in 9 populations,the lowest is Jinding, the highest is San sheldrake, which ranged from 0.5137~0.6055. The average heterozygosity of 9 duck populations was 0.5523, which reflected the rich diversity. Considerable breed differentiation was observed and 25.65% of the total genetic variation came from breed differences, this result affirmed each breed was of own genetic diversity. The DA genetic distances suggested the longer differentiation existed between those breeds. The domestic duck breeds in East China were clustered into four groups based on the NJ clustering, the clustering results had some relationship with the distributions and economic utilizations of these duck breeds.

结果表明：9个品种的平均杂合度都较高，最低的为金定鸭，最高的为山麻鸭，杂合度范围为0.5137～0.6055，群体平均杂合度为0.5523，反映了各鸭种的杂合度都较高，遗传多样性丰富；华东区各鸭种间存在较大的遗传分化，25.65%的遗传变异来源于品种间的差异，更进一步反映了各品种具有本品种特征特性的多样性；通过计算DA遗传距离发现各群体的遗传距离较远，分化时间较长；NJ聚类结果将华东区家鸭资源聚为4类，NJ的聚类结果分析与几个鸭品种的地域分布和经济用途有一定关系。

Tricholoma may be a paraphyletic genus two. The collections identified as T. caligatum is a mixture. Some parts of them from France are members of T. matsutake, the other parts from North America are closed to T. bakamatsutake and T. fulvocastaneum. Tricholoma robustum and T. focale form a clade which is obviously a sister group of the stirp Subannulate. The stirp Caligata was defined as a group mainly on the basis of the predominant veil. But from the result of molecular systematic analysis, the presence of veil is not a stable character in the matsutake group. Species having symbiosis with conifers and broad leaf tree are all transformed from those whose host specificity is not strict. The phylogenetic tree based on ITS sequences resembled the tree of NJ algorithm based on cladistic coding. In the phylogenetic tree based on morphylogical characters, T. zangii and T. bakamatsutake form a clade, but it is the result of convergent evolution as suggested by molecular analyses. The distribution area of the matsutake group can be divided into six regions: middle and northern Europe region, Mediterranean region, Hengduan Mount.— Southeastern Tibet region, Northeastern China—Japan—Far-east Russia region, the Atlantic—North America region and the Rocky Mountain region. The abundance center of the matsutake group are Hengduan Mount.—Southeastern Tibet region and the Rocky Mountain region. Hengduan Mount.

松口蘑群的分子系统学研究得出的结果有：由于粗壮口蘑和羽衣口蘑明显和亚环族(Tricholoma，section subannulate)的种类是来自同一个谱系，和其它松口蘑群的种不具有最近共同祖先，因此松口蘑群是一个多系群；松口蘑群和口蘑属其他种类的亲缘关系比和蜜环菌属更接近；梭柄松苞菇和松口蘑群的差距明显小于油黄口蘑(T.flavovirens)和棕灰口蘑；口蘑属是一个多系群；鉴定为欧洲口蘑的标本明显不是一个单系群，其中一部分是松口蘑，另一部分和黄褐口蘑及傻松口蘑(T.bakamatsutake)比较接近；划分松口蘑群的标准是菌环的存在，但从分子系统学的结果来看，菌环的存在与否并非是一个很稳定的性状；和针叶树专性共生及和阔叶树专性共生均由寄主专化性不强的种类转化而来；在形态学系统发育树上青冈口蘑和傻松口蘑被聚为一个分枝，分子系统学的研究结果表明，这是趋同进化的结果；用支序方法编码作出的NJ树的结果更接近ITS序列的结果。

XRD results indicate that the major diffraction peaks of the film match those of Sb2Te3. The film growth is apparently at the ［101］/［012］ orientation and many Te peaks are observed. Hall measurement reveals that all the samples are p-type and the resistivities are low. The electric conductivity of the films approaches that of the bulk metal and the carrier concentration is of 1023 cm-3. Seebeck coefficient measurement shows that the samples have nice thermoelectrical properties and the seebeck coefficients are in the range of 7.8—62 μV/K. Among all, the samples annealed at 200 ℃ for 6 h have the highest seebeck coefficient of about 62 μV/K and the lowest resistivity.

XRD测量结果显示，薄膜的主要衍射峰与Sb2Te3标准衍射峰相同，在［101］/［012］晶向取向明显，存在较多的Te杂质峰；霍尔系数测试结果表明，薄膜为p型半导体薄膜，薄膜电阻率较低，其电导率接近于金属电导率，载流子浓度量级为1023 cm-3，具有良好的电学性能；Seebeck系数测量结果显示，薄膜具有良好的热电性能，在不同条件下制备的薄膜的Seebeck系数在7.8—62 μV/K范围；在所制备的薄膜中，退火时间为6 h、退火温度为200 ℃的薄膜其Seebeck系数达到最大，约为62 μV/K，且电阻率最小。

Results: At five weeks, the radiographically and manually determined fusion rate for autologous bone graft was 50％(4/8animals), compared with 100％ and 50％(8/8 and 4/8 anilmals) for groups in which fusions were done by using allograft and xenograft augumented with rhBMP-2. Xenograft+HA used in posterolateral intertransverse arthrodesis yielded a fusion rate of 0％(0/8 animals).

结果：手动试验示：1、自体髂骨组即对照组融合率为50％：2、各实验组融合率分别为：同种冷冻干燥异体骨+rhBMP-2组为100％；异种冷冻干燥骨+rhBMP-2组为50％；异种冷冻干燥骨+HA组为0％。X软片示：1、自体髂骨组即对照组X线评分均值为2.75分，融合率为62.5％；2、各实验组结果分别为：同种冷冻干燥异体骨+rhBMP-2组X线评分均值为4分，融合率为100％：异种冷冻干燥骨+rhBMP-2组X线评分均值为2.62分，融合率为62.5％。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

结果：(1)通过细胞培养和MTT法，表明长白山姬松茸在体外对MGC-803细胞株有明显的抑制作用，加药组与对照组相比其生长抑制率有显著性差异(P＜0.01)，而且这种抑制作用呈现浓度和时间的依赖性；(2)通过集落形成率的测定结果表明，加药组与对照组相比其集落形成率和集落形成抑制率有明显差异(P＜0.01)，说明长白山姬松茸对MGC-803胃癌细胞株有明显抗增殖作用；(3)光镜观察结果表明，加药组可见细胞脱水浓缩伊红染色增强，胞体缩小，内含高度浓缩的胞核呈深蓝色等典型细胞凋亡形态；经AO／EB荧光染色观察结果表明，当终浓度为1.0mg／ml的姬松茸提取物作用于MGC-803胃癌细胞24h后，其凋亡率和破膜率与自然凋亡率与破膜率相比，均有显著性差异，其凋亡率86.3％(P＜0.001)，破膜率为41.6％(P＜0.01)，说明姬松茸确实有诱导MGC-803胃癌细胞凋亡的作用，同时也有细胞毒作用，但以诱导细胞凋亡为主；(4)免疫组化结果表明，用药前后凋亡相关基因的BCl-2、Bax蛋白均有显著性的改变(P＜0.001)。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。