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Src l was an acidic cytolysin, which was reported forthe first time. The cDNA encoding the mature Src l was cloned into non-fusion expression vectorpBV220 and expressed successfully in E.coli DH5α in inclusion body. After washing anddenaturing-renaturing, the recombinant Src l protein was purified by Q Sepharose FastFlow ion exchange chromatograph and Phenyl Sepharose hydrophobic interactionchromatograph.

首先构建了Src I基因的非融合蛋白表达质粒pBV220-Src I,并成功地在原核细胞E.coli进行了重组表达,筛选出稳定表达的工程菌株DH5α,摸索了稳定表达的条件,摸索了包涵体的洗涤、变性和复性条件,摸索出了复性蛋白Src I的纯化工艺,纯化过程简单,经QSepharose Fast Flow阴离子交换层析和Phenyl Sepharose疏水层析两步纯化,就可获得了高纯度的重组蛋白样品(纯度达99%以上),该纯化方法适合重组蛋白的大量纯化,为Src I的性质、结构和功能研究奠定了良好的基础。

The structure, functions, quality control and manufacturing technology of auxiliary feedwater deaerator for Qinshan Nuclear Power Station, Stage Two are described.

介绍了秦山二期核电工程辅助给水除氧器的结构形式、主要功能、质量保证体系和制造工艺。

With examples of deck pavement works, studies are carried out on the deck pavement structure and the selection of material applicable.

结合桥面铺装工程的实例,对桥面铺装结构设计和材料选配进行了探讨。

And the achievement of the demonstration project show that the software structure can realize the interconnection of the existing ITS subsystem effectively, and can be extended easily to meet the requirements of the ITS development in the future.

示范工程建设的成果表明:这种体系结构能有效地连接当前广州市多个已建ITS子系统,实现各系统数据共享和交换,同时具备良好的扩展性,能满足ITS不断发展的需求。

Based on DFP gradient method and the theory of elastic visco-plasticity and that of finite element method, the deficiency and objection existing in present stochastic back analysis methods have been deeply studied in this paper in order to provide much more reliable parameter for the mechanical analysis of geological engineering structures.

本文基于变尺度梯度优化方法和弹-粘塑性原理及其有限元基本理论,针对随机反分析方法存在的缺陷和不足进行研究,以期为实际岩土工程结构的力学分析提供更加可靠的材料参数。

At this early stage, the outline structure of the new premises will be expressed in diagrammatic form, taking into account

在此早期阶段,工程的各功能结构大纲将用图表来表示,幷将下列因素考虑在内

Based on analyzing the characteristic of Kevlar fiber composites and engineering ceramics, the diamond trepanning drill is selected, and its structure, making technology and main capability parameters are also researched and ascertained.

基于凯芙拉纤维复合材料和工程陶瓷材料的性能特点,选用了金刚石套料钻,并对刀具的结构、制作工艺、主要性能参数进行了研究和确定。

Implemented using layered heterogeneous data integrating algorithm whichbased on IP-SAN in digitized aircraft design For the advantages of IP-SAN, we provided a heterogeneous data integratingalgrithem which took IP-SAN as data center and based on layered meaning,implemented the heterogeneous data integrating system in digitized aircraft designengineer, on the basis of showing the digram of the system, we described the systemintegrating layers and characteristic.

七、提出并实现了基于IP-SAN的分层异构数据集成算法在飞机设计数字化中的应用基于IP-SAN所具有的诸多优势,提出了以IP-SAN形成数据中心,基于对象层次思想的异构数据库集成算法,实现了飞机设计数字化工程中的异构数据库集成系统,在给出系统体系框架图的基础上,描述了系统集成层次结构及特点。

This paper studies the product structure modeling and configuration on the basis of weighted digraph according to several projects about automobile manufacturing informationization, and has developed a prototype system to validate it.

本文结合多个汽车制造企业信息化建设的工程项目,研究了基于赋权有向图的产品结构建模与配置技术,并开发了原型系统进行验证。

The value of dipole moment can be conferred from the situation of the molecular symmetry, and vice versa.

偶极矩沟通了分子的微观结构与宏观性能特征,所以进行化合物偶极矩的计算和研究,具有十分重要的理论意义和工程价值。

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推荐网络例句

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里,举止就文雅一些,因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计,这件高贵的制服是香港本土制造,是我们第一套不配帽子的制服。

Do not 'inflate' your achievements and/or qualifications or skills .

不要 '夸大' 你的业绩或成果,条件或者技能。