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The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

将P3软骨细胞分为空白对照组、鹿茸多肽不同浓度组、硫酸氨基葡萄糖不同浓度组进行传代培养,同时以P2代软骨细胞为对照组,进行组化检测老化相关β-半乳糖苷酶,阿力新蓝染色检测胞外基质硫酸GAG含量和结构,MTT比色检测增殖,免疫细胞化检测PCNA和Ⅱ型胶原,RT-PCR检测Ⅱ型胶原、Aggrecan蛋白,流式细胞仪分析细胞周期和增殖指数等方法,对鹿茸多肽抗软骨细胞退变老化进行分子生物学研究。4。

Two of the most important dimensions of structure are centralization and departmentalization.

在组织结构中最关键的两个维度是集中化和部门化。

This value orientation is embodied not only in Zhang Yang's theme selection, but also in his application of languages. First, Zhang Yang's experimental chippy narration takes on the personal style. But furthermore it implies traditional linear narrative structure, respecting people's Ying-xi mentality.

首先在叙事层面,与第六代的多数导演不同,张杨的电影叙事在表层上的先锋化实验化的碎片叙事使影片呈现个人化风格,但在更深层次上却隐含着传统的线性叙事结构,尊重大众的"影戏"心理。

Microstructure analysis showed that graphitization degree of Ti-doped graphite increased and the correlation between graphitization degree and dopant quantity is complex.

微观结构分析表明,材料的石墨化程度大大提高,同时,材料的石墨化程度与其导热性能间呈现出较为复杂的关系。

In chapter 2,the main research contents and typical works in theoretical research area on amino acids and oligopeptides were introduced.This includes studies performed on structures,electronic properties,weak intramolecular interactions,protonation and deprotonation,conformer conversion,metal ion binding,solution effects and etc.

第2章给出了氨基酸和寡肽分子体系理论计算领域的主要研究内容和研究热点,这包括氨基酸和寡肽分子的稳定结构,电子性质,内部弱相互作用,质子化和去质子化过程,构象转换,金属离子结合及溶液效应等多方面的研究。

In this dissertation, systematic and extensive conformational search has been performed for asparagine and aspartic acid in gas phase and solution and the properties of the stable conformers are studied using DFT based first-principles calculation. The concerned properties include geometry structure, IR spectra, conformer distributions, intramolecular interactions, ionization energies and electron affinities, protonation and deprotonation processes.

在本论文中,我们对天冬酰胺和天冬氨酸在气相和溶液中的稳定构型进行了系统而全面的寻找,并研究了其稳定构型的几何结构、红外光谱、构型分布、分子内相互作用、电离能、电子亲合能、质子化和去质子化过程等相关性质。

We search on the courses of conjunctional grammaticalization by means of the theory of grammaticalization and methods with semantics. Then we differentiate and analyze the difference of grammatical function of synonymous conjunctions through comparison and try to give some explain in diachronic aspect. Finally we probe the laws of conjunctional grammaticalization and ways.

个案分析方面,我们运用语法化理论和方法,结合语义学、认知心理学等理论思想,从句法结构、语义变化分析的方面入手,将共时描写与历时分析相结合,试图探寻连词的语法化过程,通过比较法辨析相近连词的语法功能的差别,并试着从历史演变的角度给出解释,最后对连词演变的机制和规律进行初步归纳和总结。

The process conditions for trial production of low density polyethylene speciality resin (LD 163) for heat shrinkable film were selected after determining the speciality resin′s quality index and conjunctively based on the dependence of LDPE resin′s performance and relative mass and its distribution on the resin′s molecular chain structure, such as branched short and long chains.

确定了热收缩膜专用树脂LD163的质量指标后,根据低密度聚乙烯树脂性能与相对分子质量及其分布、短链支化、长链支化等结构的对应关系拟订了其试生产工艺条件。

The effects of FPL andits three ingredients on growth of ovary,on micro-and ultra-structure andAlkaline phosphatase activity of endometrium,on structure andkeratinization rate of exfoliated cells in vaginal epithelium in mice,onmicrocirculation in rat and on uterine kinesis in rabbit were respectivelyobserved by the techniques of light and electron microscopes,histochemistry,microcirculation and organ cinetography.The results have confirmed themechanism of FPL for the sterility caused by ovary standstill and persistentcorpus luteum to be as follows:(1) a weak estrogen-like role which canprompt development of ovary,proliferation and secretion of endometrium andvaginal epithelium and cornification of exfoliated cells in vaginal epithelium;(2)enhancing AKP's activity of endometrium;(3)improving microcirculation;(4) exciting uterus.

分别采用光学显微镜和电子显微镜技术、组织化学技术、微循环技术和器官运动描记等现代医学实验技术,观察了促孕液对小白鼠的卵巢发育、子宫内膜的显微和超微结构和碱性磷酸酶活性、阴道上皮结构和脱落细胞的角化率、对大白鼠的微循环和对家兔的子宫运动的影响,结果证实了促孕液治疗卵巢静止、持久黄体的机理:(1)微弱的雌激素样作用,能刺激卵泡发育,促进子宫内膜和阴道上皮增生、分泌和阴道上皮脱落细胞角化;(2)提高子宫内膜碱性磷酸酶的活性;(3)改善微循环;(4)促进子宫运动。

In the first place,we describe the formation for calculation of meanly minimum free energy and covariation value and how to combine them to predict the consensus secondary structure of homology RNAs.

然后讨论了具有预测伪结能力的迭代化思想,并在此基础上,提出基于平均最小自由能和协变信息预测同源RNA序列公共二级结构的迭代化方法。

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推荐网络例句

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在,'歼灭战perfuming自己与胡努特,那麼,他来到和SAT 。

The shamrock is the symbol of Ireland and of St.

三叶草是爱尔兰和圣特里克节的标志同时它的寓意是带来幸运。3片心形叶子围绕着一根断茎,深绿色。