结合量

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

The test results showed that the amount of this kind of function material to conjugate proteins were larger than 1.5ig/mm2and its one time adsorption rate was larger than 80% and can satisfy the requirements for solid-phase radioimmunoassay. Test results showed that this polymer-NHS-ester can connect with proteins through covalent bounds under mild conditions This technical method will supply a type of new material for solid-phase radioimmunoassay.

实验证实，这类聚合物NHS酯可在温和条件下与蛋白反应，使蛋白以共价键结合方式固定在聚合物上，这种功能材料对蛋白的结合量大于1.5μ／mm~2，对蛋白的一次吸附率大于80％，能满足固相放免分析的要求，将为固相放免分析提供一种新材料。

Scatchard analysis showed that one type of binding sites was formed in the imprinted polymer in the studied concentration range. The dissociation constant and the apparent maximum number of the binding sites were calculated to be 0.80 mmol/L and 52.68 μmol/g, respectively, and the maximum template selective site was 21.26 μmol/g.

Scatchard方程研究表明，在研究的浓度范围内在聚合物中形成了一类等价的结合位点，结合位点对模板分子的平衡离解常数为0.80 mmol/L，最大表观结合量为52.68 μmol/g，最大特异性吸附量为21.26 μmol/g。

Scatchard analysis demonstrates that one equal class of binding sites was formed in the imprinted polymer in the studied concentration range. The dissociation constant and the apparent maximum number of the binding sites are 1.94 mmol/L and 92.33 μmol/g, respectively.

Scatchard方程研究表明，在研究的浓度范围内聚合物中形成了一类等价的结合位点，其对模板分子的平衡离解常数K=1.94 mmol/L，最大表观结合量Cpmax=92.33 μmol/g。

Among the main proteins Cd was mainly bound with globular and glutelin.

在糙米主要蛋白中镉与谷蛋白和球蛋白结合量最多。

To understand the reaction of tetrakis hydroxymethyl phosphonium chloride with collagen protein,the interactions of THPC with polycaprolactam,poly vinyl alcohol or ethylenediamine which were used as the model substances of amido,hydroxy and amino groups of collagen protein were examed.

通过研究THPC与聚己内酰胺、聚乙烯醇、乙二胺的酰胺基、羟基、氨基的反应，间接证明THPC主要与胶原蛋白的氨基结合，可与氨基以n∶n=1.0∶2.2形成交联，THPC与羟基和酰胺基均有少量结合，并在pH=8.0时结合量最高。

Results: 125 I Mel binding sites in optomeninx was the most, in eptochiasm and sniff ball was next

结果 ：视网膜 1 2 5 I- Mel特异结合量最高，视交叉及嗅球次之，其次为下丘脑、海马、脑干；动力学分析表明 Mel与其受体的结合为可逆性结合

Results: 125 I Mel binding sites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS dose dependently inhibited the binding.

结果 ：视网膜 1 2 5 I- Mel特异结合量最高，视交叉及嗅球次之，其次为下丘脑、海马、脑干；动力学分析表明 Mel与其受体的结合为可逆性结合；特异性结合分析提示对 Mel呈高度特异性；10和 5 0μm ol/ L GTPγS使 1 2 5 I- Mel特异结合降低。

Increasing the amount of virus inoculated did not increase the virus bound to the cell. Both the number of cells and concentration of virus could influence the binding which was saturable.

增加加入JEV的量，未见到病毒的结合量有明显增加，提示这种结合是特异的，病毒浓度和细胞数量的变化对病毒与受体的结合反应有一定的影响，结合具有饱和性。

The reaction mechanism of tetrakis hydroxymethyl phosphonium chloride and raw collagen were researched by the simulated materials of collagen including glycin,ethylenediamine and hide powder.

通过研究四羟甲基氯化鏻与皮胶原模拟物反应，间接证明THPC主要与胶原蛋白的氨基结合，可与氨基以n∶n=1∶2.2形成交联，THPC与羟基和酰胺基均有少量结合，并在pH=8.0时结合量最高。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。