结合质

Methods:The expressive plasmids of AML1b and AML1-ETO were transfected into CV-1 and 293 cells.The expression level of endogenous pig7 gene was detected by Realtime-PCR.The luciferase reporter plasmids containing pig7 enhancer/corresponding mutant sequences were constructed and co-transfected into CV-1 cells with expressive plasmids of AML1b and AML1-ETO.The transactivity of pig7 enhancer was assayed by luminometer.

研究方法：AML1b和AML7-ETO表达质粒分别转染及共转染CV-1和293细胞，用Realtime-PCR方法检测上述基因对两种细胞内源性pig7基因mRNA表达水平的影响；构建含AML1b结合位点的pig7基因增强子序列以及相对应的突变位点的荧光素酶报告基因质粒；将表达载体/突变载体与AML1b或AML1-ETO基因瞬时共转染CV-1细胞，分析AML1b、AML1-ETO对报告基因的转录调节作用。

The method can be used in all kinds of domains since it calculates the global minimum of a constrained multi-extrema function to get high-quality centroidal Voronoi tessellations for the region.

该算法结合k-means方法和模拟退火算法求解约束条件下的多峰值函数全局最小值，在待求计算域内和边界自动生成无网格计算节点，且节点为计算域对应质心Voronoi结构的质心点。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Based on the short gel-time features of ordinary cement sodium-silicate and alkali-activated salic cementitious materials, an ideal structure model of high performance sodium-silicate suspension two-shot grouting material was established. An experiment was undertaken to verify the solidification value of Na~+ and water-erosion resistance of a grouting material combined with cement and metakaolin-sodium silicate. This paper put forward a compact structure with a dominant ingredient of-Si-Al-H zeolite gel formed on later age and a minor part of C-S-H formed on early age, which composed of sodium-silicate, substances that could release Ca~(2+) spontaneously and stably or by the aid of sodium-silicate, and active salic substances.

结合普通水泥-水玻璃快速胶凝和碱激发硅铝质胶凝材料的特点，本文建立了高性能水玻璃悬浊型双液灌浆材料的理想结构模型，并通过水泥复合偏高岭土-水玻璃灌浆材料的Na~+固化性能和抗水溶蚀性能进行了试验验证，提出其组成结构应是以-Si-Al-H类沸石凝胶物质为主、C-S-H为辅的复合共存密实结构，其组成要素为水玻璃、能自发稳定地或在水玻璃作用下迅速释放出Ca~(2+)的物质、活性硅铝质材料。

A silicon based piezo-electricity socle girder miniature electric generating apparatus is provided with a silica fixing frame, an oxidizing layer, a platinum/titanium lower metallic layer, a piezo-electricity PZT layer, a platinum/titanium upper metallic layer, an electrode insulation protection layer, and a vibrating base; the structure of the silica fixing frame combines the monocrystal silicon wet method etching and dry-etching to process, whose structure is integrated as a whole, and the center part of the structure is in a socle girder shape, which includes a silicium socle girder and a free end mass block; the piezo-electricity power supply device uses a micro-machining technique to integrally machine, and can realize the integration of the power supply and the microelectronic devices.

一种硅基压电悬臂梁微型发电装置，有硅质固定框架1、氧化层2、铂/钛下金属层3、压电PZT层4、铂/钛上金属层5、电极绝缘保护层6、振动底座7构成；硅质固定框架1的结构是利用单晶硅湿法腐蚀和干法腐蚀相结合完成加工，其结构连为一体，其中心部分的结构为悬臂梁形状，包括硅悬臂梁a和自由端质量块b；压电电源装置利用微加工工艺集成加工，能够很好的实现电源、微电子器件集成。

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

These two series of probes differ in their mechanism of action. The first series exploits the concept of suicide substrate. When the probe is hydrolyzed by the target sulfatase, it will release an intermediate that undergoes elimination to form a reactive quinone methide which in turn reacts with nearby nucleophiles. The second series belongs to an electrophilic reagent which utilizes a cyclic sulfamate moiety serving both as the recognition unit and the trapping device.

这二系列标示分子的主要差异在於其标示机制，第一系列是源自於自杀性受质的概念，利用水解后的中间体会生成高反应性的quinone methide，来达到标示的效果；第二系列则是属於亲核性试剂型，采用环状胺基磺酸衍生物做为辨识端及捕捉机制，预期在其进入酵素的受质结合区后能与酵素催化中心形成共价键结。

In order to improve the hydrothermal stability of leather,two kinds of tanning agents with weak properties were used in combination tannage .

利用两种鞣性比较弱的鞣质进行结合鞣制，得到的成革的耐湿热稳定性大幅度提高，原因是在这两种鞣质之间形成了协同效应，即Synergistic Effect，这是提高成革稳定性的一个比较新型的研究理论体系。

The petrophysical properties reflect reservoir heterogeneity by integration of well testing with reservoir performance are acquired by means of geostatistics , multi-parameter cluster analysis , neural network technique , a set of method of quantitative division and estimation to reservoir quality unit in carbonate pools of fracture and vug type is explored , the estimated parameter for different unit is acquired. which lead to better the understanding of reservoir behavior and heterogeneity.

以地质统计学技术、神经网络技术和多参数聚类分析技术为手段，利用试井资料及其它开发动态资料，动静态资料相结合，研究和求取了反映岩溶储层非质性的物性参数；研究和探索了一套缝洞型碳酸盐岩储层开发储集质量单元的定量划分和评价方法，建立了不同单元的储层质量评价参数，深化了对缝洞型碳酸盐岩储层非均质性的认识。

Spikelets all alike, somewhat laterally compressed, with 1 to several fertile florets; rachilla usually disarticulating below each floret, occasionally strictly 3 florets with lower 2 staminate or barren and of different appearance from upper fertile floret, then disarticulating above glumes and florets falling together; glumes persistent, often equal to spikelet or at least longer than first floret, commonly membranous with broad, shining, hyaline margins; floret callus bearded; lemmas hyaline to leathery, 3- to several-veined ( Coleanthus 1-veined), apex entire or denticulate, awned from back, rarely awnless; awn usually geniculate with twisted column; palea hyaline, subequaling or much shorter than lemma.

全部一样的小穗，有点侧面压扁，具1到数个能育的小花；脱节的通常小穗轴在下面每小花，偶尔严格加雄蕊或者无意义的更低的2的3小花和上面肥沃小花的不同外表，然后脱节的上述颖片和小花结合起来；宿存的颖片，等于小穗或长于前小花，宽的膜质具，发亮，透明边缘的通常的至少的通常；公然反对的小花结茧；透明的外稃到革质，3-到数枚脉(Coleanthus 1脉)，先端全缘或具小齿，从背面，很少awnless那里具芒；用盘旋的柱子通常膝曲的芒；透明的内稃，近相等或者远短于外稃。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。