结合质

The rheological characteristics of an artificial synovia mainly consisting of hyaluronic acid prepared by editors was discussed.

研究以透明质酸为主要成分的人工滑液的流变性质，分析添加γ－球蛋白和La－DPPC 磷脂对人工滑液流变性能和润滑性能的影响，并结合滑液粘弹性质与摩擦副表面理化性质对润滑模式进行了探讨。

Sp were evaluated by computer-assisted image analysis software, and the content of OPG determined by immunohistochemistry, which included mean optical density 、positive area density in bone trabecula and total positive area of cancellous bone, were evaluated by computer-assisted image analysis and semiquantitative analysis.

Sp，免疫组化方法结合图像分析半定量分析OPG在胫骨的表达，测定平均光密度、OPG在骨小梁中阳性表达的面积密度和OPG在松质骨骨组织阳性表达的总面积。

Differentially expressed protein spots were identified by Tandem MS as RNA binding protein regulatory subunit, Proteasome subunit beta type 7 precursor, and Translationally controlled tumor protein.

进一步选择了3个组间差异表达蛋白点进行串联质谱分析，分别鉴定为RNA结合蛋白调节亚基、蛋白酶体β亚基7型前体和翻译调控肿瘤蛋白。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

SUMO has been shown to covalently modify a large number of proteins, reversible modification by SUMO regulates nucleocytoplasmic translocalization, protein-DNA binding activity, protein-protein interaction, transcriptional regulation, DNA repair, and genome organization.

SUMO与靶蛋白之间这种可逆的共价连接，在核质运输、DNA 与蛋白质结合活性、蛋白质之间相互作用、转录调控、DNA 修复以及维持基因组稳定等方面均发挥着重要的调节作用。

As we show in detail in Figure 29.31, the result of Wnt signalling is to increase the level of -catenin, which causes it to translocate to the nucleus.

Ras固定在细胞膜里面向细胞质的一侧，以活化的Ras与Ser/Thr激酶Raf结合，因而将Raf带到质膜，使得Raf被激活。

Methods The epithelial cells, which were taken from neonate Wistar rat, were cultured into tubiform epithelium together with the frame of shell polysaccharide matrix in vitro.

用Wistar大鼠乳鼠皮肤为细胞来源，结合管状壳多糖基质网架，在体外共同培养成复层皮肤结构，移植到裸鼠皮下。分别于移植的5、7、14、28、35 d收取标本，光镜下观察移植物生长情况。

At the super-low permeable reservior sandstone of Fu and Yang in Daqing Oilfield, many pore throats exist a lot in the types of compacted and restored flakes and curved lamellas while the pore volume of micropore and the thin, compacted and shrunken throat that contribute little to flow take large scale in the total pore space. Thereof high filtrational resistance is caused by much trouble including high content of reservoir shale, serious sensibility to pressure at waterflooding, remarkable effects of undersaturation and water blocks in micropore. A method with laboratory experiment, theoretical confirmation and field application included was adopted to analyze the non-linear flow characteristics, to reveal the deep mechanism of pinhole enveloping macropore and to provide a new technology of improving the development effect. The outcomes aquired are as follows: Multiple cores with different low and super-low permeability were selected to perform seepage experiments by gas, water and oil. Three fields of flow were observed in the reservoir at different low and super-low core permeability. They are pseudo- elastic flow, transition flow and plastic creep flow. So the concept that there was no correlation of rocky absolute permeability with fluid properties that pasted itself was broken up. Two-phase seepage of water displacing oil in uniform super-low permeability shows that the two-phase seepage zone is narrow.

针对大庆油田扶、杨特低渗透油层砂岩孔隙喉道的大小和形态以压实再生型片状、弯片状喉道分布较广，压实、缩小型细喉道型，微孔不可流动的孔隙体积所占总孔隙体积的比例较大的特点；及储层泥质含量高，注水开发中压力敏感性强、微孔隙欠饱和及水锁效应显著，导致流体渗流阻力增大的问题，本文采用室内实验、理论推证和矿场应用相结合的方法，研究、分析了流体非线性渗流特征，更深刻地揭示出小孔包围大孔的机理及产生原因，并提供了改善开发效果的新的技术方法，取得如下成果：选择不同渗透率的低渗透、特低渗透岩心，进行了气、水和油单相渗流实验，揭示出当岩心渗透率不同时，油层内的流体呈现出拟弹性流变、过渡流变、塑性蠕变三种流变区，从而打破了岩心的绝对渗透率与通过岩心流体性质无关的观点；同类特低渗透岩心的水驱油两相渗流实验表明，两相渗流区较窄。

Furthermore, preliminary work also performed to examine whether PI3K/AKT signal transduction pathway was activated in the process of refractory leukemia development. Materials and methods An immortalized human bone marrow stromal cell line, HS-5, was introduced to establish a bi-phase culture system for the cultivation of B-lineage precursor leukemia cells. ELISA and RT-PCR were used to investigate the expression of VEGF and its receptors in the leukemia cell lines and primary childhood leukemia cells in different treated groups. Flow cytometory method and immunofluorescent staining were employed to examine the apoptosis signals both in the VP16 treated and untreated leukemia cells. Western blot was utilized to explore the PI3K/AKT activated status in the drug induced or uninduced leukemia cells and lymphocytes from healthy donors.

材料和方法使用来源于人类骨髓基质细胞的细胞株HS-5作为滋养层细胞进行急性淋巴细胞性白血病细胞的体外培养，通过细胞生物学和免疫学方法评估培养体系并鉴定出难治性白血病细胞克隆；以ELISA和RT-PCR方法检测急性白血病细胞株和患儿白血病细胞VEGF及其受体的表达，了解不同治疗阶段VEGF及其受体的表达状况，并结合临床指标进行分析，明确VEGF及其受体在白血病发生过程中的作用；流式细胞仪和免疫荧光染色法对正常健康儿童、初发白血病患儿、复发白血病患儿及缓解后患儿进行凋亡因子检测和分析，初步阐明难治性白血病抗凋亡形成的原因；蛋白印记分析检测PI3K/AKT信号传导通路在健康儿童、初发白血病和复发白血病患儿的表达，初步了解难治性白血病形成的分子生物学机制。

We also compared the rejoining ability of DSBs in both unirradiated and irradiated H1299 cells by using a DSB rejoining assay.

我们也利用DNA双股断裂再结合线性冷光报导质体之能力的实验来比较细胞株在有无游离辐射处理。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。