结合质

With the method of multitube electrode recording, the transmitter characteristics of LA inhibiting the acoustic response of neurons in A-Ⅰ has been studied.

七运用多管微电极记录方法结合微电泳技术，探讨了LA抑制皮层AⅠ神经元声反应的递质特性。

S100β is one kind of the calcium binding proteins. As growth factor of neuraxon, it is excreted by neuroglial cell, and distributing in nerve tissue extensively.

S100β是一类钙离子结合蛋白，是轴突的生长因子，由神经系统胶质细胞分泌，广泛分布于神经组织中，在正常情况下发挥重要的生理作用，但分泌过高具有神经毒性。

In the C-terminal of the protein, there is a 40% homology to olfactomedin, which is a major component of the extracellular matrix of the olfactory neuroepithelium. The region includes all reported TIGR gene mutations, which indicates that it is associated with cellular binding activity.

在蛋白的 C-末端部分在一个区域与嗅觉介导素40%同源，嗅觉介导素是嗅神经上皮的细胞外基质的一种主要成分，这个区域含有所有已经报告的 TIGR 基因突变，提示其与细胞结合活性有关。

The high velocity arc spraying technology and powder-cored metal threads were bi-used to repair the waste components, and the integration between coating preparation and materials formation to Fe-Al base intermetallic compounds came true. A new supersonic ion spraying technique, named bi-channel, bi-temperature, was invented to solve successfully the issues of over-melting, half-cooked and burned materials. The high-energy mechanical and chemical method with own property right was explored to solve successfully the issue, which the nano particles dispersed evenly and suspended stably in the solution. After perfecting the techniques the high-performance co-electrodeposition between the conductive matrixes metal Ni and the nonconductive nano particles carried out. The excellent nano anti-friction self-repairing additives was prepared to achieve elementarily the self-repairing on the worn surface during the equipment worked.

首次将高速电弧喷涂技术与粉芯丝材相结合的方法应用于再制造零部件的表面修复，实现了Fe-Al金属间化合物的制备与涂层成形一体化；发明了一种&双通道、双温区&的高效能超音速等离子喷涂新工艺，解决了过熔、夹生及烧损问题；开发了具有自主知识产权的高能机械化学法，解决了纳米电刷镀时纳米颗粒在多离子溶液体系中的均匀分散与悬浮稳定的难题，通过工艺优化实现了非导电的纳米颗粒与导电的基质金属镍的高效共沉积；发明并制备了性能优异的纳米减摩自修复添加剂，初步实现了在装备运行过程中对磨损部位的原位动态自修复。

The results calculated by the Elovich rate equation showed that K release rates from 1 mol.L-1 HNO3 extraction ＞those from 2 mol.L-1 HNO3 extraction ＞those from H-resin extraction. Elovich equations combining with the data of H-resin and 2 mol.L-1 HNO3 successive extraction could well described the release characteristics of soil K. The correlation coefficients of the equations reached most significant levels (r＝0.956**—0.999**), and standard errors of specific K release capacities between the vaules calculated by the equations and those measured by both successive extractions were smaller(s=1.61 and 3.52). The specific release rates of soil K calculated by the Elovich rate equations were significantly correlated with the indices of soil K availability (the amounts of easily available K, nonexchangeable K, total available K in soils and K absorbed by ryegrass).

研究结果表明：1 mol.L-1 HNO3提取法的释K速率＞2 mol.L-1 HNO3提取法的释K速率＞氢饱和交换树脂提取法的释K速率；氢质交换树脂和2 mol.L-1 HNO3提取法结合Elovich模型较理想地描述了土壤K素的释放特性，拟合方程的相关系数均达极显著水平(r＝0.956**-0.999**)，土壤特征累积释K量的计算值与实测值的标准差较小(s=1.61和3.52)，且通过速率方程计算的土壤特征释K速率与K素有效性指标(速效K、缓效 K、有效K及黑麦草总吸K量)的相关性也较为密切。

Glioma is still one of refractory disease in the neurosurgical field; the development of new primary and adjuvant treatment is vital. Recently, the gene therapy of glioma is developed rapidly and there are many methods about the gene therapy that include: suicide gene therapy, immunologic gene therapy, drug resistangce gene therapy, angiostatin gene therapy and so on. The sucide gene therapy is the most potential approach of antitumer, these nonmammalian genes encode enzyme that convert nontoxic prodrugs into highly toxic metablites. Cells transfected with suicide genes are targeted for specific negative selection, witch can be induced by administrtion of the corresponding produg. Among the enzyme/produg combinations, two of the best characterized system are herpes simplex virus thymidine kinase /ganciclovir and Escherichia coli cytosine deaminase /5-flourocytosine (5-FC). The formor can convert the antiviral nucleoside analogs acyclovir , ganciclovir to their nucleoside monophosphate derivatives, the monophosphate forms are subsequently phosphorylated by endogenus cellular kinases to triphosphates, these molecules are potent inhibitors of DNA synthesis.

近年来脑胶质瘤的基因治疗发展迅速，应运而生的方法有自杀基因、免疫基因、多药耐药基因以及抗血管生成基因等，其中自杀基因被认为是最有前景的基因治疗方法，它又称病毒介导的酶／药物前体疗法，是利用转基因技术将哺乳动物细胞中所不含有的自杀基因转入到哺乳动物肿瘤细胞中，该基因表达的产物可将无毒的药物前体转化为毒性药物，从而选择性杀伤该肿瘤细胞，常用的自杀基因有单纯疱疹病毒-胸苷激酶基因和大肠杆菌胞嘧啶脱氨酶基因，前者催化无毒性抗病毒核苷类似物如丙氧鸟苷、无环鸟苷等成为单磷酸核苷衍生物，然后在内源性细胞激酶作用下转化为具有明显毒性的三磷酸核苷，作为DNA合成链的终止剂和DNA合成酶的抑制剂，干扰细胞DNA的合成；后者编码的胞嘧啶脱氨酶可催化5-氟胞嘧啶（5-FC）脱氨成为5-氟尿嘧啶（5-FU），然后代谢为有毒性的5-氟尿嘧啶-5′三磷酸（5-FUTP）和5-氟-2′脱氧尿嘧啶-5′磷酸（5-FdUTP），5-FUTP通过与UTP竞争性结合而抑制mRNA和tRNA的合成，5-FdUTP则作用于胸苷合成酶，导致TMP衰竭而阻止DNA的合成，最终诱导肿瘤细胞凋亡。

DH level, CD4+ T lymphocyte count below 100 × 106/L, and interstitial pneumonia or ground-glass shadow in chest images, the diagnosis of PCP could be made presumptively. It is difficult to make a nosogenic diagnosis of PCP, but TBB considerably increases the positive rate of pneumocystis. Compound sulfamethoxazole is recommended as the first selected drug. In severe cases, corticosteroid and assisted mechanical ventilation combined with compound sulfamethoxazole could remarkably improve the prognosis of PCP.

当AIDS患者出现发热、咳嗽、呼吸困难、低氧血症、LDH增高及CD4+T淋巴细胞＜100×106/L时，结合胸部影像间质性肺炎或磨砂玻璃样改变，临床需考虑PCP；病原学诊断困难，但TBB肺孢子菌检出率高；治疗首选SMZ-TMP，重症病例辅以糖皮质激素和机械辅助通气可改善预后。

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

Curran and a team of American and Canadian researchers, in conjunction with the Philadelphia-based Radiation Therapy Oncology Group, a federally-supported clinical trials organization, found that among 289 patients with either anaplastic oligodendroglioma or anaplastic oligoastrocytoma receiving either a combination of three chemotherapy drugs with radiation or radiation alone, those whose tumors had deletions in chromosome locations 1p and 19q tended to live nearly two and a half times longer than those patients without the missing chromosome portions.

临床癌症杂志》上周报道，卡仑博士和由美国、加拿大研究人员组成的科研小组，联合了总部设在费城的由联邦政府支持的临床实验组织－美国放射肿瘤学协作组，进行了研究。289名病人患有退行性少树突神经胶质瘤或退行性少星细胞瘤，研究发现，这些病人在接受三种化疗药物结合放疗或者只接受放疗后，其中染色体缺乏1p 和19q 的病人比那些不缺乏的病人多存活了2倍半的时间。

Here, similarities between archaebacteria and eukarykaryotic otic cells in many aspacts including DNA duplication, transcription, translation and histones, chromatin, proteasome were introduced. Finally, the evolutionary position of archaebacteria and their significance to onery gin of the eukaryotic cells were discussed.

结合国际上对原细菌，尤其是它与真核细胞在许多方面（包括DNA复制、转录和翻译整个中心法则，组蛋白、染色质及蛋白酶复合体等）密切的亲缘关系的研究结果，对原细菌的进化地位及其对揭示真核细胞的起源进化问题的意义进行了探讨。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。