结合质

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

The dopamine release in vivo was monitored by fast cyclic voltammetry and the concentrations of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic acid were determined by high performance liquid chromatography electrochemical detection in the process study. In addition, the effects of some traditional Chinese herbs and related marine drug on behavioral changes were also evaluated in order to provide some suggestive clue for rational drug design of anti-Parkinon's disease .

许多因素都参与PD的DA能神经元的死亡，所以，本研究在制备PD动物模型的基础上，应用国内率先引进的快速周期伏安法检测设备在体动态监测DA的释放，同时应用另一种电化学检测技术—高效液相色谱电化学检测法离体检测DA及其代谢产物二羟苯乙酸和高香草酸的含量，结合行为学测定及其它方法，研究了雌激素和铁在PD发病中的作用，以及一些药物，如吲哚醌、牛磺酸、铁离子螯合剂、胆囊收缩素（CCK-8）、乌鸡白凤丸有效成份、大豆异黄酮及海洋酸性寡糖HSH971，对DA能神经元的影响，从DA能神经递质角度对PD的发病机理提供进一步的实验依据，并为PD的预防和治疗提供新的干预措施。

Results CsA clearly decreased creatinine clearance and increased urinary excretion of N-acetyl-β-D-glucosaminidase in rats as compared with those in the vehicle group. Morphologic changes consisted of vacuolization of tubular cell, expansion of the interstitium and interstitial fibrosis, hyalinization of afferent arterioles. Severity of the injury was related to the dosage of CsA. Striped interstitial fibrosis and arteriolopathy were more severe in the rats treated with CsA and low-salt diet than with CsA and normal diet.

结果 用CsA的大鼠出现尿量增多、内生肌酐清除率下降、尿N-乙酰-β-D-氨基葡萄糖苷酶排泄增多等；病理上表现为不同程度的细胞浊肿、空泡变性及灶性分布的间质纤维化和小动脉病变，随CsA剂量的增加，病变呈加重趋势；结合低钠饮食，CsA慢性肾毒性病变明显加重。

The detection of apoptotic cells and expression of the hyaluronic acid binding CD44-receptor were determined by flow cytometry.

细胞的增殖通过3H-脱氧胸苷酸掺入的方法检测；细胞凋亡及透明质酸结合受体CD44的表达用流式细胞仪检测。

The calculating formula used for the intumescent velocity was presented and was applied to the model proposed.

同时考虑传质传热过程并结合膨胀速度的经验公式，建立了膨胀阻燃聚合物燃烧的数学模型。

The author consider that the market of hygienic food has the severest problem, customers are deluged with all kind of inveracious advertising and their benefit is damaged by those distorted information.

表现在广告无信息量和无信息质上，并阐述由此带来的后果。笔者结合实际分析得出，信息不对称现象最严重，问题最为突出的就是中国的保健品市场。

So far this method has been successfully applied to HPLC analysis of simple monosaccharides. A baseline separation of five monosaccharides was obtained under optimized isocratic condition. Both UV detector and electrospray ionization-Mass spectrometry were selected to determine the monosaccharide composition in real samples.

结合建立的衍生化方法与高效液相色谱HPLC，优化分离条件，并选择紫外和电喷雾—离子阱质谱两种检测模式对实际样品中所含的单糖进行分离分析，建立了5种单糖的常规定性定量方法。

The nature of the sorbate/framework can be identified from the A_T values, isosteric heat and interaction energy.

发现烷烃类、胺类、芳香族化合物都对全硅β沸石骨架表现出亲和性，而醇类由于羟基的存在表现出对骨架的憎性，结合吸附热等数据讨论了各类吸附质与骨架相互作用的性质。

Combined with available data, it is comidered that the sourcerock of biotite--leptynite might be intermediate-acid volcanic material of ca.2.7 Ga, and that of migmatized leptite and quartzite might come from granitic crust older than 3.0 Ga. The iron formation was formed on the continental crust base at about 2.7 Ga, and was immediately metamorphosed for the first time.

结合已有的研究资料可以认为，黑云变粒岩的原岩来自2.7 Ga左右的中酸性火山物质，浅粒岩和石英岩的原岩来自3.0 Ga以上的花岗质地壳物质；含铁建造形成于陆壳基底之上，形成时间约2.7 Ga，并于2.5—2.7 Ga之间发生首次变质作用。

According to distribution of bryophytes on different substances, the frequencies of Bazzania albifolia, Heteroscyphus coalitus and Homaliodendron scalpellifolium in evergreen broadleaved forests of Daweishan Nature Reserve are highest; those of Macrothamnium macrocarpum, Bazzania fauriana, Ptychanthus striatus, Pogonatum cirratum and Thuidium cymbifolium are higher. Additionally, Aerobryum speciosum, Trachycladiella sparsa, Taxiphyllum subarcuatum, Aerobryidium levieri, Lepidozia wallichiana, and Leucobryum bowringii are relatively common.

根据大围山常绿阔叶林内苔藓植物在不同基质上的生长分布情况，结合其甲凡J}rg另出现的频度可知白叶鞭苔、双齿异粤苔、以及刀叶树平醉是大围山常绿阔叶林内的三大优势种；南木醉、厚角鞭苔、皱尊苔、刺边小金发鲜和大羽醉在大围山常绿阔叶林内出现的频率也很高；此外气鲜、散生粗带醉、钝叶鳞叶鲜、莱氏毛扭醉、瓦氏指叶苔、以及狭叶白发鲜等在大围山常绿阔叶林内也有一定数量的分布。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。