结合质

OBJECTIVE: Western-blot technique is utilized to further validate FHL1 and HOXD13 gene expression in protein levels in muscle tissue of patients with congenital clubfoot, the in vivo HOXD13 and predicted binding sites in embryonic foot development was verified using chromatin immunoprecipitation technology.

目的：应用Western-blot技术进一步验证先天性马蹄内翻足患者足部肌肉组织中FHL1，HOXD13基因蛋白质水平的表达，应用染色质免疫沉淀技术验证在胚胎足部发育时在体内HOXD13和预测结合位点的结合作用。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence, the coding sequence of flavine adenine dinucleotide binding domain and nicotinamide adenine dinucleotide binding domain of AIF protein.

在AIF△1-120基因克隆成功的基础上进一步改造，构建截去AIF基因线粒体定位信号，FAD结合结构域和NADH结合结构域（1-400位氨基酸）编码序列的AIF△1-400基因，将其克隆入pcDNA3和pIRES2EGFP真核表达载体，用脂质体法转染HeLa细胞，通过荧光显微镜观察，MTT检测等方法检测该基因在转染细胞中的表达及其对肿瘤细胞的促凋亡活性。

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

Through the test of lightweight infilled material in multi-ribbed wall structure,a axial compression constitutive model of lightweight infilled material at the(6±2)% water content is proposed.

通过对密肋复合墙体结构常用的轻质填充材料在含水率为(6±2)%时的单轴受压试验，确定了密肋复合墙体结构的轻质填充材料单轴受压本构模型，进而基于统一强度理论，结合抗拉强度及抗剪强度试验，确定了密肋复合墙体结构填充材料的多轴破坏准则，并建立了密肋复合墙体填充材料的弹塑性本构模型。

Introduce powder material from prefix = st1 ns ="urn:schemas-microsoft-com:office:smarttags" Korea and combine liquid foundation as smooth as silk with natural plant extractions. Then bake it under high temperature to remove extra water and make delicate powders as light as air.

引进韩国粉质原料，将如丝般柔滑的粉底原液和天然植物萃取精华相结合，置于高温环境下烘焙，蒸发多余水分，制造出如空气感般轻盈的细腻粉质。

node location is one of the key technologies in wireless sensor network.rssi－based location is a hotspot in nowadays.for resolving biggish error in rssi－based location,the paper presents a new method of location,rssi－based triangle and centroid location,using triangle and centroid method to reduce the error of rssi measurement.simulation experiments prove that this algorithm can obviously improve the location accuracy compared to trilateration.

摘 要：节点定位是无线传感器网络中的关键技术之一。基于rssi的定位技术是现阶段研究的热点，为解决rssi测量方法定位误差较大的问题，提出一种将rssi测量方法与三角形质心算法相结合的新型定位算法，该算法用三角形质心算法减小rssi的测量误差。仿真表明该算法比基于rssi的三边测量法定位算法的定位精度有较大提高。

The expression of nestin protein which is the mark of neural stem cells had been found in SD rat organ of Corti on postnatal day 16 in aminoglycoside group, while no expression on the same day in normal group.

银染法不支持新细胞是小胶质细胞的假说；结合以往免疫组化(白细胞共同抗原LCA，CD45)研究的阴性结果，认为可以初步排除小胶质细胞的可能。

Recent evidence indicates, however, that the hardness of hard wheat comes from greater continuity of the protein matrix within the cells and the tighter bonding of starch granules to this matrix.

然而最近有迹象表明，硬质小麦的硬性是由于细胞内部蛋白质基质的连续程度较大和淀粉该基质的结合较牢而产生的。

The quantitative and qualitative methodologies were both used in this thesis, andby presently observing of English Immersion classroom teaching and video taperecording, a lot of information about teacher-child speech interaction was collected. Theinternational Child Language Data Exchange System, known as CHILDES, wasadopted to analyze the collected information qualitatively and quantitatively.

本项研究采用量化研究和质性研究相结合的方法，通过对幼儿园大、中、小班英语浸入式教学活动的现场观察和录像收集师幼言语互动语料，运用国际通用的儿童语言数据交流系统——CHILDES，对收集到的师幼互动交流言语数据进行统计分析，在对研究资料进行量化分析的同时，对研究结果进行质性的分析和讨论，并提出了相应的教学建议。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。