结合物

This is due to the fact that AFP in the sample flows through the sample pad and binds the conjugate to form a complex. This complex then migrates down to the membrane and binds to the immobilized anti-AFP antibody A2 of the test line, resulting in the immobilization of the reddish conjugate.

如果样品中含有甲胎蛋白，膜上便会出现肉眼可见的微红色检测线，这是由于样品中的AFP与标记有单克隆抗体A1的免疫金结合形成复合物，复合物向膜上移动与固定在膜上的检测线单克隆抗体A2结合反应，结果在该处形成固定的微红色线条。

The interactions of dsDNA and ssDNA with two osmium complexes have been studied by surface electrochemical methods based on DNA modified electrode. It is found that both osmium complexes bond DNA with electrostatic interaction. And some thermodynamics and kinetics constants, such as bindind constant , ratio of binding constants K〓/K〓, limiting ratio K〓°/K〓°at zero ionic strength (μ＝0), free energy ΔG〓, dissociation rate constant k and binding site size s have been obtained.

研究发现两种锇配合物都是通过静电作用与DNA结合，并计算得到联吡啶锇和二氯菲咯啉锇与dsDNA和ssDNA相互作用的结合常数、结合常数比K〓／K〓、μ＝0时极限结合常数比K〓°／K〓°、结合自由能ΔG〓、解离速度常数k和结合位点数s等多个热力学和动力学参数。

They have close relationship as the former may provide new ideas and approaches for the later, while the later may be the simulation for the former and gives it helps in degradation products identification and mechanism explanation. It is necessary to choose a suitable system, which has high potential of treating EDs and close relationship to the natural environment, to combine the researches in the two aspects. Our group has been engaged in researches on the photochemical properties and application to water pollution control of different ferric species including Fe -OH complexes, Fe -polycarboxylate complexes, Ferric oxides and Fe -humic acid complexes. Fe -oxalate complexes system has higher efficiency than the other ferric species systems in photooxidation and degradation of organic compounds. This thesis is focused on the photodegradation of nine EDs estradiol (E2), estrone (E1), 17α-ethynyl estradiol (EE2), diethylstilbestrol , bisphenol-A , 4-Nonylphenol , 4-Octylphenol , di-n-butyl phthalate , and di (2-ethylhexyl) phthalate .

因此从处理效率和环境化学意义两方面考虑，结合本课题组在不同铁形态（包括Fe-OH配合物、Fe-多羧酸配合物、Fe氧化物、Fe-腐殖酸配合物等）的光化学特性及其在水污染控制中的应用方面较系统的研究工作基础，本论文选择了四类共9种典型的内分泌干扰物（雌二醇（E2）、雌酮（E1）、17α-乙炔基雌二醇（EE2）己雌酚、双酚A、壬基酚、辛基酚、酞酸二丁酯和酞酸二乙基己基酯），研究了它们在Fe-草酸盐配合物体系中的光降解效果，推测其降解机理，探索环境内分泌干扰物光氧化处理的新体系，同时为预测其在环境中的光降解行为提供了依据。

The dsDNA and ssDNA modified electrodes were prepared by directadsorption method, and were used to study the interactions with metal complexes atthe electrode surface. The binding mechanism, binding parameters and electrochemicaldata were also obtained by this micromethod.

4采用直接吸附法制备了ssDNA或dsDNA修饰电极，研究了各配合物和ssDNA或dsDNA在电极表面的相互作用，讨论了结合机理，得到了配合物和dsDNA或ssDNA在电极表面的结合参数。

As metal ions can interact with both of ADM and DNA, they undoubtedly affect the interaction between ADM and DNA. Addition of a large excess of Cu or Fe into a solution of well-formed ADM-DNA complex makes the metal ions simultaneously bind to ADM and DNA to form a ternary complex. Otherwise,〓 or 〓 complexes can bind directly to DNA to form a ternary complex.

金属离子-ADM-DNA三元体系的光谱研究表明，当ADM与DNA结合后，大量Cu或Fe的加入可以使金属离子进入到ADM-DNA复合物中，并同时与ADM和DNA键合，形成三元复合物；反之，若ADM先与Cu或Fe配位，生成的金属配合物则能够直接与DNA结合形成三元复合物。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

According to Johnson's Rule and IR spectra, their possible structures are suggested. The bonding parameters of those complexes are calculated by ESR parameters. And the crystal field parameters are calculated by using electronic spectral data. It can be seen that the vanadyl complexes in the solution are ionic complexes and that the α〓 values of those complexes decrease following the increasing of the N donor replacing the O donor of water on the equatorial plane in those complexes. This means that the covalent bonding between vanadium and ligand increases as Ser, Phen or Bipy replace water ligands. The results show the competitive coordinate law that the coordination reactivity of Phen and Bipy is much stronger than that of Ser. The simulation program of ESR is compiled by the theory of ESR in the present work.

测定了VO-丝氨酸二元体系、VO-丝氨酸-邻菲咯啉三元体系和VO-丝氨酸联吡啶三元体系在不同酸度下（pH=1.0～14.0）的乙二醇／水（V／V=1：1）溶液低温ESR谱，发现不同pH下，溶液中具有不同组成的配合物，根据不同pH下ESR谱的变化，利用Johnson的加合规则并结合红外光谱，推测了它们在溶液中的可能结构；利用波谱参数计算了配合物键参数；利用电子光谱数据计算了配合物的晶体场参数，讨沦了它们的成配特性，结果表明：VO配合物属离子型配合物，随取代水分子的配体（Ser、Phen或Bipy）数目增加，键参数减小，配合物共价性增强，同时得出这些体系中，生物配体竞争配位规律：Phen和Bipy与VO的配位能力比Ser强。

In this paper, the stable Au complexes are classified according to the coordination atoms and their characteristic structure. The recent development of the researches on the antitumor activity of Au complexes including the structure-activity relationship, biological targets and the mechanisms of action are reviewed. The structure of the coordination ligand and the leaving group have great impact on the in vitro cytotoxicity of the Au complex. Many physical and biological methods are introduced to study the interaction between the Au complexes and the suspected bio-target, such as DNA, protein, mitochondrion, thio-containing biomolecules, etc. The interaction modes (intercalating, electrostatic interaction, covalent bonding, etc.) are focused to illustrate the reason for the antitumor activity of Au complexes.

本文按配位原子的不同总结了稳定三价金配合物的结构特征，按其生物活性的构效关系、生物靶点和作用机制综述了三价金配合物抗肿瘤活性研究的最新成果：配体的结构特点以及离去基团对三价金配合物的体外细胞毒性影响较大；介绍了用于检测三价金配合物与可能的生物靶分子之间的相互作用的多种物理和生物学方法，重点关注了相互作用的模式，如嵌入/静电吸引/共价结合等，并解释了三价金配合物抗肿瘤活性的原因。

In this paper, the stable Au complexes are classified according to the coordination atoms and their structural characteristics. The recent development of the researches on the antitumor activity of Au complexes including the structure-activity relationships, biological targets and the mechanisms of action are reviewed. The structure of the coordination ligand and the leaving group have great impact on the in vitro cytotoxicity of the Au complex. Many physical and biological methods are introduced to study the interaction between the Au complexe and the suspected bio-target, such as DNA, protein, mitochondrion, thio-containing biomolecules, etc. The interaction modes (intercalating, electrostatic interaction, covalent bonding, etc.) are focused to illustrate the reason for the antitumor activity of Au complexes.

本文按配位原子的不同总结了稳定三价金配合物的结构特征，按其生物活性的构效关系、生物靶点和作用机制综述了三价金配合物抗肿瘤活性研究的最新成果：配体的结构特点以及离去基团对三价金配合物的体外细胞毒性影响较大；介绍了用于检测三价金配合物与可能的生物靶分子之间相互作用的多种物理和生物学方法，重点关注了相互作用的模式，如嵌入/静电吸引/共价结合等，并解释了三价金配合物抗肿瘤活性的原因。

Based on the structure, those human diseases related mutation sites are mapped onto the structure accurately. As a result, it is found that these sites are all around the electron transfer prosthetic groups or ubiquinone. And these mutations will alter prosthetic groups' binding or ubiquinone's binding, causing electron leakage from the prosthetic groups in the electron transfer chain, producing reactive oxygen species and leading to tumor formation.

利用线粒体复合物 II 的结构对已知与人类疾病相关的该复合物突变位点进行了精确定位，发现这些突变位点均位于电子传递体或辅酶 Q 结合位点的周围，它们的突变或影响了电子传递体的结合，或影响了辅酶 Q 的结合，从而导致电子传递的中断，电子逃逸到线粒体基质中或线粒体内膜中，经过一系列下游途径导致疾病的产生。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。