结合物

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The results presented here demonstrate that the highly specificantibody-antigen interaction can be used to generate single-moleculemaps of specific types of molecules in a compositionally complexsample while simultaneously carrying out high-resolution topographicimaging.

为了能够在新模型中应用原子力显微镜，他们在原子力显微镜的尖端粘上针对单个蛋白的抗体。为了保证抗体探针是真正敏感的，用一个多聚物把抗体和尖端连接起来，使抗体摆动到与蛋白受体更易结合的位置。

Dual wavelength method and Balance dialysis method were expounded and the experimental results were consentaneous compared with molar wavelength ratio.

研究发现，PVP-BPB缔合物的结合比为nBPB∶nPVP=1∶10，表观摩尔吸光系数ε=4.55×104L/，PVP与BPB作用的条件平衡常数K=4.32×103。

Two-dimensional electrophoresis (2-DE) was used to study the variation expression of proteins of cucumber cotyledonary nodes induced with PP333. The characteristics and functions of differentially expressed proteins were analyzed with MALDI-TOF-TOF/MS and Protein Data Bank.

应用双向凝胶电泳技术研究PP333处理后黄瓜离体子叶节培养物差异蛋白的表达，结合质谱分析和蛋白质数据库检索，确定所检出差异蛋白质的性质和功能。

Group IV: Polyalphaolefins are made by combining 2 to more decene molecules into an oligomer, a short-chain-length polymer.

第四组：聚的是由结合2更癸分子成低聚物，短长链聚合物。

Double chain naphthyurea/thiourea piperazine derivatives could act as a fluorescent chemosensor not for aromatic dicarboxylate but for linear dicarboxylate especially for pimelate anion.

双链萘脲基或硫脲基哌嗪化合物与直链二羧酸盐络合具有较高的稳定常数和1∶1的化学计量比，两者通过六个氢键形成双点结合的络合物。

Access to the catalytic center and an unusual switch I movement in the dimeric structure support this finding.

二聚体中催化中心的入口和一种不寻常的开关运动支持了这一猜想，即易位子复合物之间的相互作用或蛋白质前体的潜在结合位点都是由这种二聚体的结构特点决定的。

These results show that the complex is essentially electrostatically bound to DNA.

所有以上实验结果说明，该配合物主要是通过正负电荷间的静电作用与DNA结合。

For the stepwise mechanism, the ethylene molecule was firstly adsorbed at the Brnsted acid site via π-hydrogen bonding to form a stable complex, which then went through the protonation, leading to the formation of the ethoxide intermediate. Furthermore, the ethoxide reacted with the second ethylene molecule to form the butoxide product.

对于分步反应机理，乙烯分子首先通过π-氢键作用在酸性位形成稳定的吸附络合物，再进一步发生质子化并生成乙醇盐中间体，随后乙醇盐与第二个乙烯分子发生碳－碳键结合形成丁醇盐产物。

The 648 bp mitochondrial cytochrome c oxidase subunit I gene was examined in 8 species of raptors (5 species in Accipitridae, Falconiformes; 3 species in Strigidae, Strigiformes) by PCR with universal primers. 99 additional barcode sequences were downloaded to explore whether the DNA barcodes could distinguish between these species.

用DNA条形码通用引物扩增了我国8种猛禽（14只个体）的线粒体细胞色素c氧化酶亚基I基因，选取648 bp DNA条形码标准区域，结合99条猛禽条形码序列，探讨DNA条形码对猛禽的识别和鉴定。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。