结合

Chapter 5, from a cognitive point of view and integrating the analytical points covered in the previous chapters, examines the metaphoric nature of intentional humor which serves in a sub-discourse as ideational and/or interpersonal means of rhetoric under the global discourse of public speaking, with case analyses of such humorous metaphors, standard or non-standard, at the word, sentence, and discourse levels as well as at the meta-level, and with the idea of resemblance proximity to classify humorous metaphors into synecdochic, metonymic and metaphorical types.

第五章从认知语义的角度并结合前三章的分析来讨论公众演说者有意幽默的隐喻性，考察标准和非标准幽默隐喻在词汇、句子、话语层面以及在元层面的表现，并结合演说语料的个案分析考察幽默隐喻作为次话语在演说整体话语中所达到的概念修辞和人际修辞效果。基于隐喻的相似度，本章还将幽默隐喻进一步分类为借代性幽默、换喻性幽默，和隐喻性幽默。

The contents of different forms of nitrogen and phosphorus in different sites were mainly influenced by terrigenous inputs, sediment grain size and hydro-dynamics.

表层沉积物中总磷的含量平均值为455.94mg/kg；有机磷占总磷含量的39.41%，平均含量为179.69mg/kg；铁结合磷和铝结合磷平均含量为94.97mg/kg。

The combation of disparity consistency and matching cost are used to detect occlusion area. Fast and effective image segmentation is applied for extracting textureless and texture regions. The matching problem of textureless regions is then solved with constraint of disparity consistency and credibility concept. At the meantime, high credible seeds are obtained. Based on the smooth constraint of matching and disparity of seeds, dense disparity map is obtained via the adaptive-window based region growing.

然后采用视差一致性和匹配代价相结合的方法来检测遮挡区域；采用一种快速有效的图像分割算法提取了低纹理区和纹理区域，结合视差一致性和置信度约束来有效地解决低纹理区的匹配问题，同时得到一系列具有高置信度的种子点；根据立体匹配中的平滑性约束和种子点的视差，利用自适应窗口的区域增长技术最终得到致密的视差图。

Both the thermophilous plants and hardy plants should be planted to keep the operation of the wetland system all tear round and the submerged plants and the emerging plants should be planted in a same space to make the wetland system more effectively.

在时间上，可将喜温植物与耐寒植物结合，以保证湿地系统的长年运行；在空间上，可将挺水植物与沉水植物结合，以保证湿地系统的处理效果。

The composition, interface and cross section of the sintered EPCD coating were observedand analyzed by SEM, X-ray diffraction , Infra-red spectrum, EPMA, Energydispersion X-ray fluorescence analysis and X-ray-electron spectroscopy,the results show that the coating made from〓BG is composed of HA, titanite and bioglass.

钛合金基体表面的喷砂处理不仅可改善与涂层的机械结合，还可加速其表面氧化和降低预氧化温度，对于实现涂层的低温快速烧结和提高涂层结合强度有重要意义。

The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection. A signal significantly greater than background binding was observed from the third to the fifth round of selection on CHOEGFRGFP1 and CHOK1 cell, but a part of polyclonal scFv bound EGFR specially. About 45% of the selected clones contained a fullsized insert of 1 kb. One unique human antiEGFR scFv (F4scFv) was isolated by analyzing with cell ELISA and DNA sequencing.

结果 经过5轮筛选，细胞裂解液中洗脱出噬菌体效价有500倍以上增长；细胞ELISA结果显示多克隆pscFv与CHOEGFRGFP1细胞和CHOK1细胞均有明显结合，但有部分特异性结合EGFR；菌落PCR显示约45％克隆中含有完整的1kb scFv片断；经细胞ELISA、 DNA测序检测共获得1株EGFR特异性单链抗体，命名为F4scFv。

Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

The UV/vis exhibited the absorbance of BSA(210nm) to shift tored and decrease gradually with the addition of nicotine, reflecting the transition of secondarystructure of BSA, namely the helix of BSA become looser.

Lineweaver-Burk 双倒数方程计算pH7.4 和 pH11.0 体系在温度为 20℃和 37℃条件下尼古丁和 BSA 的结合常数 k 分别为： k20℃=140.15 L·mol-1 ，k37℃=131.83 L·mol-1 （pH7.4）和 k20℃=141.76 L·mol-1，k20℃=27.79L·mol-1(pH11.0)，表明结合常数在 pH7.4 条件下受温度的影响要比 pH11.0 条件下小，推测是由于不同 pH 下尼古丁存在的不同形态所致。

In the dynamic test program, the idea of"software is instrument"was applied to meet the diverse test demands. Last, it is proposed that the of VXI test system be developed to more totalization and networked. The basic theory of system identification was introduced briefly at first.

并结合自动测试系统的发展，研究了VXI测试系统与网络技术结合，实现各种软硬件资源共享，进一步提高设备及数据的利用率和系统的扩展性，使自动测试向综合化、网络化方向发展。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。