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Studies of the binding of metal to calmodulin and the subsequent binding of metal-calmodulin complexes to the target protein/peptide revealed that the La-content calmodulin complexes to its target exhibit similar affinity with the Ca-calmodulin complexes.However, there were substle differences in protein conformation as well as the kinetical properties between the two kinds of metal-calmodulin complexes, suggesting Ln could produce cellular effects via the Ca/calmodulin signaling systems.

稀土离子和钙调蛋白的结合以及后续和靶酶的结合,在热力学上与钙离子激活蛋白具有相同的亲合力,但在动力学性质上以及细微构象上明显不同,显示稀土离子可通过细胞钙相关信号系统产生特别的生物效应。

Electronic Science and Technology, China, Sichuan University, No.611 Institute, Sichuan Hospital, Provincial Hospital, Provincial Knub Hospital, etc.Chengdu Zhongxuan Digital Entertainment Co., Ltd

这种中外结合并产学研相结合的研发机构设置,不仅使公司在技术上始终和世界保持同步,而且不断为公司培养出世界一流的放疗产品研发杰出人才。

Considering the merits of in vivo studies in biotransformation i. e. activation or inactivation, protein-binding such as sex hormone binding protein , toxicokinetics, and more evaluative in hazard identification and risk assessment, we selected a battery of short-term and long-term in vivo studies, including uterotrophic assay in weaning mice and ovariectomized adult mice, an in vivo multiple endpoints assay, and a gestational and lactational exposure assay to identify the estrogenic effects of endosulfan on the offspring.

考虑到体内试验在代谢转化、血浆蛋白如性激素结合蛋白的结合、代谢动力学等方面比体外试验优越,在危害鉴定和危险度评定中更有价值,我们选择了多项体内试验,包括对幼年小鼠和卵巢切除成年小鼠的子宫增殖作用、对成年去势小鼠的体内多终点观察以及大鼠孕期和哺乳期接触对雄性子代生殖系统结构和功能的改变,即去雄性化或雌性化作用等,来验证硫丹的体内雌激素样作用。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示,甲醛刺激后19个蛋白斑点发生变化,肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点,已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ,磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6,这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Eleven subcloned DNA fragments from the 5′- upstream region of lipA, lipC and lipF of Phanerochaete chrysosporium were assayed by using the gel mobility shift assay. The total proteins extracted from P.chrysosporium mycelia grown in Kirk medium and natural fir wood chip were used to identify the segments in these 11 DNA fragments which are controlled by some regulatory proteins.

用DNA-蛋白质体外结合实验和凝胶迁移率变动分析技术筛选黄孢原毛平革菌木质素过氧化物酶基因lipA、lipC、lipF的5′-端调控区内能与该菌在木质素降解条件下形成的蛋白特异结合的顺式作用元件。

SDS could dissolve the protein envelop as well as the lipide membrane of pathogen and cell. SDS is applied in light industry widely and safely, SDS is also made as microbicides in topical vaginal formulations to prevent the transmission of herpes and possibly other pathogens that cause sexually transmitted diseases,including HSV.

十二烷基硫酸钠(Sodium dodecyl sulfate,SDS)是一种阴离子型表面活性剂,为生物膜的最有效的溶解剂,它可以同病菌和各种高等动物的细胞膜的磷脂等脂质体结合而溶解,与蛋白质结合而变性。

Dynamic Mechanical Analysisis one ofdynamic thermal machinism analyse technology,measuring changes of viscoelastical parameters alongwith temperature or frequency under vibrative load.DMA estimates various performances of viscoelasticalmaterial,using rheological index.

简要阐述了DMA的原理,论述了用DMA方法进行沥青材料性能研究的优越性,结合具体实例阐述该方法在研究沥青及沥青结合料的粘弹性、高温性能、疲劳性能和低温性能等方面的应用,以及DMA方法用于测定沥青零剪切粘度的方法介绍。

Dynamic magnetic therapy: combination of traditional Chinese medicine health study, use of modern magnetism of the latest achievements in overcoming the traditional static magnetic magnetotherapy all the disadvantages of making full use of dynamic alternating magnetic field, greatly increased the permeability of the magnetic field so that The role of magnetic field greatly increased the depth, combined with mechanical massage and physical therapy to achieve the role of three-dimensional.

动磁疗法:结合中医养生学,运用现代磁学的最新成果,克服了传统磁疗中静磁的各种弊端,充分利用动磁的交变场效应,大大增强磁场的渗透性,使磁场的作用深度大大增强,同时结合机械按摩,达到立体理疗的作用。

With strong economic and technological strength; company owns international top-level body underwear designer - Mr. Peng Xiuhong, he has toured Japan to engage in body underwear design studies for many years, he is the most authoritative body design instructor, combined with domestic Humanistic design style, and reasonable utilization study the human body, mechanics, and engineering, combined with high elastic fabric°¨of the three-dimensional cutting, the human body to achieve a revolutionary breakthrough to enable women to maintain a perfect body no longer subject to take medicine, injections, acupuncture, the pain of chemotherapy, and non-toxic side effects, and on Body Underwear magnet clamp on health care, the five points on the human body positioning magnetotherapy Massage, can prevent breast cancer, cervical cancer, suspended aging, and promote the body metabolism.

具有雄厚的经济和技术实力;公司拥有国际顶级美体内衣设计师--彭修宏先生,他曾赴日本从事美体内衣设计深造多年,他是国内最具权威性的美体设计导师,结合国内人文设计风格,合理利用人体学、力学、和工程学,结合高弹性杜邦莱卡布料的立体性裁剪,使人类美体达到了革命性的突破,使女性保持完美体形不再受吃药、打针、针灸、化疗的痛苦,并无毒副作用,并在美体内衣上钳上保健磁石,对人体的五大穴位进行定位磁疗按摩,可预防乳腺癌,宫颈癌,缓期衰老,促进人体新陈代谢。

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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。

Closes the eye, the deep breathing, all no longer are the dreams as if......

关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。