结合

The successive stability constants which are obtained by non-linear least-squares methord fitting Bjerrum formula. In three- system ,the results from Scatchard plots indicate that there exists two strong binding site of Mn and one strong binding site of Zn,there are same results compareing with two -system ,but some more weak binding sites are different from the different addition sequence ,show that there are not common strong binding site of Mn,Zn in HSA or BSA .Strong binding sites have electiveness and unfalteringness ,weak binding sites exists irreversibility.

用非线性最小二乘法拟合Bjerrum方程，计算了同时竞争体系的逐级稳定常数；Scatchard图分析表明，三元体系中，Mn和Zn与血清白蛋白结强合部位数分别为2和1，与二元体系相比，保持不变，弱结合部位数随加入顺序的不同而不同，但都有所增加，竞争结果说明了Mn和Zn与血清白蛋白结合没有共同的强结合部位，强结合部位具有专一性，选择性，而弱结合部位具有不可逆性。

Firstly, the paper, combining the characteristic of synchronous pulse bursts and inhibition with the modified PCNN model, presents a way of finding the foveation points in the images adaptively and effectively, and simulates the human vision system. Secondly, PCNN is extended to PCNNs, based on the properties of information couple and transmission, an algorithm that is used to fuse images of the same target got by several sensors to an image is presented to simulate the human vision system. Thirdly, combining the properties of synchronous pulse bursts, capture, and transmission and competition of waves, the paper presents two ways of classification, one is an algorithm based on the properties of neuron to capture and inhibit to classify the data taking on any complex unlinear distribution robustly, the other is based on the restricted distance and modified of the former to remove the influence of inferior samples in classification; Finally, based on the accumulative difference pictures, and the forming and transmission of PCNN wave, selecting and controlling the direction of autowave by connecting the neighbouring neurons selectively, the paper presents a way to simulate the tracks of moving object and detect the moving direction.

首先结合PCNN的同步脉冲发放和侧抑制特性，提出了基于改进型PCNN的图像凹点检测算法，该算法是一种自适应而有效的图像凹点检测方法，并且较好地仿真了人类视觉系统；然后，结合信息传递和信息耦合特性，将PCNN扩展成PCNNs，提出了一种基于PCNNs的图像融合算法，能够将多个传感器获取的同一目标的图像信息融合到一幅图像中，有效模拟了人类视觉系统；另外，结合PCNN的同步脉冲发放特性、捕获特性和波的传播竞争特性，开拓地将PCNN用于模式分类中，提出了基于耦合神经元点火捕获／抑制特性的分类方法和改进的约束距离下的PCNN分类方法，前者可实现对样本空间中任意复杂分布训练样本的稳健非线性分类，而后者能够消除训练样本中刺点对分类的影响；最后，结合累积差分图像思想、PCNN波的形成与传播特性，通过各神经元之间连接取向来选择与控制自动波的流向，将PCNN用于运动视觉分析中的运动轨迹模拟及运动方向检测。

An antibody binding to IGF-IR and inhibiting the binding of IGF-I and IGF-II to IGF-IR which is characterized in that said antibody is a is ofIgG1 isotype, b shows a ratio of IC50 values of inhibition of the binding of IGF-I to IGF-IR to the inhibition of binding of IG-II to IGF-IR of 1:3 to 3:1, c inhibits for at least 80% at a concentration of 5 nM IGF-IR phospohrylation in a cellular phosphorylation assay using 3T3 cells providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0.5% heat inactivated fetal calf serum when compared to such an assay without said antibody, and d shows no IGF-IR stimulating activity measured as IGF-IR phophorylation at a concentration of 10 M in a cellular phosphorylation assay using 3T3 providing 400,000 to 600,000 molecules IGF-IR per cell in a medium containing 0,5% heat inactivated fetal calf serum when compared to such an assay without said antibody has improved properties in antitumor therapy.

一种已经提高了抗肿瘤治疗的特性的抗体，所述抗体结合IGF-IR并且抑制IGF-I和IGF-II与IGF-IR结合，其特征在于所述抗体a是IgG1同种型，b显示其对IGF-I与IGF-IR结合的抑制的IC 50 值与其对IGF-II与IGF-IR结合的抑制的IC 50 值的比率为1∶3-3∶1，c当与没有所述抗体的这种测定比较时，其在5nM的浓度上，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3细胞的细胞磷酸化测定中，抑制至少80％的IGF-IR磷酸化，所述3T3细胞提供400，000-600，000分子IGF-IR/细胞，和d当与没有所述抗体的这种测定相比，在包含0.5％的热灭活胎牛血清的培养基中，使用3T3的细胞磷酸化测定中，其在10μM的浓度上没有显示作为IGF-IR磷酸化所测量的IGF-IR刺激活性，所述3T3提供400，000-600，000分子IGF-IR/细胞。

The results indicated that the extraction rate of mineral from pollen in 70℃ water was the highest. the extraction rates of four mineral: Fe, Zn, Cu and Mn were 6. 931％, 52. 786％, 44. 443％ and 46. 549％ respectively. Second speciation presented in the form of exchangeable and nonexchangeable speciations, organic and inorganic speciations at the same time. The contents of both Fe and Mn in nonexchangeable speciation were more than 50％, most contents of Fe、 Zn、Cu、Mn were inorganic speciation. Among the four elements, the content of protein binding speciation of Fe was the highest: 47. 305％. The average mineral contents of soluble carbohydrate and grease binding speciation were less than protein binding speciation.

结果表明：70℃水浸泡花粉，矿物质浸出率最高，Fe、Zn、Cu、Mn四种元素总提取率分别为6.93l％、52.786％、44.443％和46.549％；次级形态以稳定态与不稳定态、有机态与无机态等多种形态共存，其中Fe、Mn元素不稳定态含量均大于50％，Cu、Zn、Mn、Fe元素大部分以无机态形式存在；四元素中Fe元素蛋白质结合态最高，达47.305％，可溶性糖类结合态及脂肪结合态平均含量比蛋白质结合态平均含量少。

The antibody is bound to an inert polymer support ,then exposed to reacts with the antigen at a different epitope is added. The second antibody used is one that has an enzyme attached to it that coverts a colorless or nonfluorescent substrate into a colored or fluorescent product. The amount of second antibody bound, and hence the amount of protein antigen present in the original sample, is determined by quantification of the intensity of color or fluorescence produced.

将抗体结合在一惰性聚合物载体上，然后与样品接触，洗脱未经结合的蛋白质加入与抗原其他表位也可以结合的另一种抗体，在所用的第二抗体上结合有酶，此酶可把无色的或无荧光的底物转化有色或显示荧光的产物。

Increasing the amount of virus inoculated did not increase the virus bound to the cell. Both the number of cells and concentration of virus could influence the binding which was saturable.

增加加入JEV的量，未见到病毒的结合量有明显增加，提示这种结合是特异的，病毒浓度和细胞数量的变化对病毒与受体的结合反应有一定的影响，结合具有饱和性。

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

The binding behavior and kinetics of uncoupling protein with its ligand, the Fourier-transformation infrared spectra of uncoupling protein and its changes during ligand-binding process, the inhibition of Guanosine 5＇-Tetraphosphate (G-4-P) and Adenine-β-Darabinofuranoside 5＇-triphosphate on the binding of uncoupling protein with GTP were studied.

本文对解偶联蛋白与配基的结合及其结合动力学，解偶联蛋白及其与配基结合过程中的傅里叶变换红外光谱及其光谱变化，鸟嘌呤核苷四磷酸（G-4-P）和9-β-D-阿拉伯糖基呋喃5＇-三磷酸对解偶联蛋白与GTP结合的影响进行了研究。

The compensation system of first-line workers of state-own coal enterprise was redesigned under the direction of compens

结合相关薪酬理论，对国有煤炭企业一线员工的薪酬体系进行了全新设计，构建了由基本薪酬、效率薪酬、安全薪酬及福利薪酬四大部分组成的以激励为导向的&绩效薪酬体系&，详细论述了各大组成部分的设计方法和设计步骤：综合考虑企业生存状况和社会收入状况，采用以劳动定额和社会平均收入相互平衡的方法，设计出一线员工的基本薪酬模块；提出了用专家评议法确定施工难度等级指标体系的相关权重、用综合模糊评价法来确定作业难度等级、把团队人力资源要素与作业区域的施工难度状况紧密结合起来测算团队业绩，构建出体现团队业绩和贡献的效率薪酬模块；构建了以&安全抵押&制度为核心，以月度安全收入、季度安全收入、年度安全收入为框架，形成了短期、中期与长期安全收入相结合的安全薪酬模块；结合国有煤炭企业一线员工来源复杂、身份复杂、需求不同的现状，采用了员工参与性和自决性极强的&自助式&福利薪酬模式。

The combined acids , LHZZS,XDZZS and SLZZS, one of four fractions in montan resins from montan waxes from Liaohu, Xundian and Shulan brown coal respectively, were derived by CH 2N 2 to methyl esters and their chemical constituents and structural characteristic were studied on GC-MS.

结果显示，树脂结合酸均以正构烷酸为主要成份，其分布为C1 2 ～C2 8，高含量的正构烷酸集中在低碳数一端，C2 0 含量最高，其次为C1 6 ，而舒兰结合酸中，正构烷酸占绝对优势，且C1 6 为最高含量的化合物；去氢松香酸在每一个样品中含量较低，其它三环二萜酸，如松脂酸、三达松脂酸等异构体或立体异构体含量也非常低；具有五环三萜骨架的酸性物质，在潦浒、舒兰结合酸中比较丰富，其含量超过寻甸结合酸；在低碳数一端，存在着各种支链烷酸及苯甲酸、苯酚及取代物。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。