结合

In the methods of research, this paper pays attention to the combination of demology, economics and sociology etc, uses the scientific elements in the existing research results, and unites it with the reality of China closely. In the background of dualistic structure, it emphasizes particularly on the rural old-age security issues to do an intersectional research. It combines the theory with the reality, combines the qualitative research and quantitative research. The countermeasures are realistic, feasible and far-sighted.

在研究方法上，本论文注重人口学、经济学、社会学等理论方法的结合，合理借鉴国内外已有研究成果科学成份，紧密结合中国实际，在"二元结构"大背景下，侧重对农村养老保障问题进行交叉研究，做到理论与实际想结合，定性与定量分析相结合，对策研究注重现实性、可行性和前瞻性。

This dissertation adopts an approach of positivism and integrates the synchronic and diachronic, the phonetics and phonology as well as qualitative and quantitative so as to observe, analyze and describe the diphthong in a vision of historical typology.

本文立足于共时和历时相结合，宏观和微观相结合，语音学和音系学相结合，定性研究和定量研究相结合的研究方法，寄希望于在未来的时间里采用更好的方法来解决研究中发现的某些问题。

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

We first studied the interaction mechanism of Erythrosin B with proteins by spectrophotometry and fluorospectrophotometry, calculated their binding number, binding constants and binding site, and estimated their binding type.

将分光光度法和荧光光度法结合，首次研究了四碘荧光素与蛋白质的作用机理，计算了结合数、结合常数和结合位置，判断出结合类型。

The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm，但由于颗粒表面本身带负电，不能包括有效地结合及保护DNA；经Ca~(2+)修饰后，颗粒可带正电，可一定程度地结合和保护DNA，但聚合物本身的中性电与带负电的细胞膜结合，而且本身的颗粒则变地容易聚集而成为很大的颗粒，也不利于细胞的吞噬；经PLL修饰后，颗粒溶解性提高，直径变小，表面带正电，电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏，而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

Results: 125 I Mel binding sites in optomeninx was the most, in eptochiasm and sniff ball was next; GTPγS dose dependently inhibited the binding.

结果 ：视网膜 1 2 5 I- Mel特异结合量最高，视交叉及嗅球次之，其次为下丘脑、海马、脑干；动力学分析表明 Mel与其受体的结合为可逆性结合；特异性结合分析提示对 Mel呈高度特异性；10和 5 0μm ol/ L GTPγS使 1 2 5 I- Mel特异结合降低。

The thought political work has the substantivity and the indirect disposition union,the individual and the social disposition union,the microscopic value and the macroscopic value unifies,the spiritual achievement and the material achievement unifies five major characteristics which as well as the qualitative appraisal and the quantitative evaluation wants to unify,according to the thought political work quantification appraisal selection principle,proposes the thought political work quantification content and the appraisal means.

思想政治工作量具有直接性与间接性相结合、个体性和社会性相结合、微观价值和宏观价值相结合、精神成果和物质成果相结合、定性评价和定量评价相结合的五大特点，本文根据思想政治工作量化考核的选取原则，提出思想政治工作成果量化的内容和考核办法。

The results indicated that the chemical fraction composition characteristics of heavy metals are different. Cu mainly existed in organic/sulphidic fraction and residual fraction; Pb mainly existed in carbonates, Fe-Mn oxides and residual fraction; Cd mainly existed in carbonates and Fe-Mn oxides fraction; Cr mainly existed in residual fraction.

结果表明，不同重金属在消落区沉积物中形态分布各异，Cu主要以有机物及硫化物结合态和残渣态形式存在，Pb主要以碳酸盐结合态、Fe-Mn氧化物结合态及残渣态存在，Cd主要以碳酸盐结合态、Fe-Mn氧化物结合态存在，Cr主要以残渣态存在。

The gene-expressionprofile might help to further understand the molecular basis of tube worm physiology.It will also lay a good foundation for functional studies on the adaptation to extremeenvironments and help to make use of them. MicroRNAsare a class of small noncoding RNA that are 20-22nucleotidesand function as regulators of gene expression.

其中，几丁质结合蛋白最具多样性，不仅几丁质酶水解区和几丁质结合区相互独立，而且几丁质结合区也显示丰富的多样性，由不同的几丁质结合区共组成了23种不同的几丁质结合蛋白；EST测序分析中还发现两个溶菌酶基因带有双功能区，可能具有双重功能；此外，肌红蛋白基因也显示了序列的多样性。

In order to escplore the new models of cultivating in school, the essay tightly connects cultivating with teaching and research activities, based on developing teachers vacational level by modern information technoledge. Besides, during the process of founding the system of trainning teachers in school, we decide on our cultive target—promote the element educational reform by school internet, combing the facts of different schools. The cultive contnet should suit measures to different conditions, trying to synthesise and stress its practical function. When carrying out the cultive plan, we should sum up different models and explain its essence, steps and function so that we make the best choice. As to evaluational methods, we can take varied evaluational ways, including self-evaluation and evaluating each other by combination of fixing quality and quantity. At last, it is very necessary to found a special organization in order to form cultive web, make entire standarization, pay attention to develop resource and provide cultive guarantee.

本文在构建网络环境下中小学教师校本培训体系时，把以教师专业发展为本，以学校为主要研究基地，以现代信息技术为手段，以新课程的学习和实施为抓手，把培训和教研活动紧密结合、溶为一体，探索校本培训的新模式作为目标；把以基础教育新课程改革为出发点，在总目标的框架下，结合学校的具体实际，以校园网络为依托，来确定相应的培训任务，以促进基础教育课程改革的顺利进行作为任务；培训内容因人而异、因校而异，力求综合化，强调实用性与实践性；就相关的网络环境下校本培训模式进行了归纳整理，就每种模式的基本内涵、实施步骤加以说明，并对其功能列表进行比较，以便使用时更好地选择；运用多元评价和多样化评价方式，采取定性和定量相结合、自评和互评相结合、学校整体和教师个人发展相结合等灵活多样的评价方法，认真地进行科学的全方位的评价；建立一个专门的机构进行组织与管理：建立组织机构，形成培训网络，进行整体规划，优化培训机制，注重资源开发，提供培训保障。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！